Cell Function Using NOD SCID Xenografts

使用 NOD SCID 异种移植物的细胞功能

• 批准号: 7642431
• 负责人: KAREN E. POLLOCK
• 金额: $ 9.6万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7642431
• 关键词: Academic Medical Centers; Bone Marrow; Breeding; Cell physiology; Cells; Cellular biology; Data; Development; Diabetes Mellitus; Experimental Designs; Funding; Future; Generations; Goals; Harvest; Hematology; Hematopoietic; Hematopoietic stem cells; Housing; Human; Human Resources; IACUC; Indiana; Individual; Institutes; Investigation; Kidney Diseases; Laboratories; Medical; Molecular; Mus; National Institute of Diabetes and Digestive and Kidney Diseases; Non obese; Numbers; Participant; Phenotype; Program Research Project Grants; Protocols documentation; Relative (related person); Research; Research Personnel; Resources; SCID Mice; Savings; Science; Services; Source; Spleen; Stem cells; T-Lymphocyte; Tissues; Training; Transplantation; Treatment Protocols; Vendor; Xenograft procedure; animal resource; boys; cost; design; experience; gene therapy; in vivo; peripheral blood; programs; ranpirnase; research study; stem; tissue processing

A shared "Mouse Core" is proposed as Core Unit C for the program project. The goal of this core is to enable the investigator to perform in vivo studies in a timely and economical manner. The responsibilities of this core are two-fold. First, this core will maintain breeding colonies for B6.SJL-PtrcaPep3b/BoyJ (Boy.!) mice and NOD.CB17-Pr/¿fciC"'/J-nonobese/severe combined immunodeficient-(NOD/SCID) mice. Separate breeding and experimental rooms for these colonies are conveniently located in the adjacent Medical Sciences buildir g in the Laboratory of Animal Resource Center. The BoyJ mice will be used in a variety of transplantation experiments in which the relative repopulating ability of two genetically distinct sources of stern culls will be studied. In addition these mice will be used to study submyeloablative regimens and strategies to select for low numbers of transduced stem cells in vivo. The NOD/SCID mice will be utilized in investigations focused on human stem and progenitor cell biology in this program. Second, for NOD/SCID experiments, the core leader will manage and coordinate with core personnel all aspects of the transplantation. In addition, core personnel will perform transplants into NOD/SCID mice, harvest and provide tissues from NOD/SCID experiments to investigators of this program project for analysis. This will also be coordinated with the "Cell Purification and Analysis Laboratory (Core A)." The core leader will also provide assistance with experimental design and interpretation of data generated in NOD/SCID xeriograft transplantation experiments. The shared Core A will provide participants in the program project with costeffective and high quality services for their research. These studies will provide new information regarding hematopoietic stem-cell function in vivo that is applicable to the future development of gene therapy trials.

建议将共享的“鼠标核心”作为该计划项目的核心单元 C。该核心的目标是 使研究者能够及时、经济地进行体内研究。的职责 这个核心有两个方面。首先，该核心将维持 B6.SJL-PtrcaPep3b/BoyJ（男孩！）的繁殖群体 小鼠和 NOD.CB17-Pr/¿fciC"'/J-非肥胖/严重联合免疫缺陷-(NOD/SCID) 小鼠。分开 这些菌落的繁殖室和实验室位于邻近的医学科学中心，交通便利 动物资源中心实验室建设。 BoyJ 小鼠将用于多种领域 移植实验，其中两种遗传上不同的来源的相对繁殖能力 将研究严格剔除。此外，这些小鼠将用于研究亚清髓疗法和 选择体内少量转导干细胞的策略。 NOD/SCID 小鼠将用于 该项目的研究重点是人类干细胞和祖细胞生物学。二、对于NOD/SCID 实验中，核心领导负责与核心人员进行各方面的管理和协调 移植。此外，核心人员还将对NOD/SCID小鼠进行移植、收获和 向该计划项目的研究人员提供 NOD/SCID 实验的组织进行分析。这将 还与“细胞纯化和分析实验室（核心A）”进行协调。核心领导也将 为 NOD/SCID 异种移植物中生成的数据的实验设计和解释提供帮助 移植实验。共享的核心 A 将为计划项目的参与者提供具有成本效益的 并为他们的研究提供高质量的服务。这些研究将提供有关以下方面的新信息： 体内造血干细胞功能，适用于未来基因治疗试验的发展。