Cell Function Using NOD SCID Xenografts

一个共享的“鼠标核心”被提议作为该计划项目的核心单元C。这个核心的目标是 使研究人员能够以及时和经济的方式进行体内研究。的责任 这个核心有两个方面。首先，这个核心将保持B6.SJL-PtrcaPep3b/BoyJ(男孩！)的繁殖群体。 小鼠和NOD.CB17-Pr/？FCIC“‘/J-非肥胖/严重联合免疫缺陷-(NOD/SCID)小鼠。分离 这些菌落的繁育和实验室位于邻近的医学科学区，位置便利 动物资源中心实验室建设。BoyJ鼠标将用于各种 在移植实验中，两个不同的遗传来源的相对再繁殖能力 将对斯特恩捕杀进行研究。此外，这些小鼠将被用于研究亚清髓性方案和 选择体内低数量转导干细胞的策略。NOD/SCID小鼠将用于 在这个项目中，研究的重点是人类干细胞和祖细胞生物学。第二，用于NOD/SCID 实验中，核心领导将管理和协调核心人员的方方面面 移植。此外，核心人员将进行NOD/SCID小鼠移植，收获和 将NOD/SCID实验的组织提供给该计划项目的研究人员进行分析。这将是 还与“细胞纯化和分析实验室(核心A)”协调。核心领导人还将 协助对NOD/SCID干法移植产生的数据进行实验设计和解释 移植实验。共享核心A将为计划项目的参与者提供具有成本效益的 以及为他们的研究提供高质量的服务。这些研究将提供关于以下方面的新信息 体内造血干细胞功能，适用于未来开展基因治疗试验。

A shared "Mouse Core" is proposed as Core Unit C for the program project. The goal of this core is to enable the investigator to perform in vivo studies in a timely and economical manner. The responsibilities of this core are two-fold. First, this core will maintain breeding colonies for B6.SJL-PtrcaPep3b/BoyJ (Boy.!) mice and NOD.CB17-Pr/¿fciC"'/J-nonobese/severe combined immunodeficient-(NOD/SCID) mice. Separate breeding and experimental rooms for these colonies are conveniently located in the adjacent Medical Sciences buildir g in the Laboratory of Animal Resource Center. The BoyJ mice will be used in a variety of transplantation experiments in which the relative repopulating ability of two genetically distinct sources of stern culls will be studied. In addition these mice will be used to study submyeloablative regimens and strategies to select for low numbers of transduced stem cells in vivo. The NOD/SCID mice will be utilized in investigations focused on human stem and progenitor cell biology in this program. Second, for NOD/SCID experiments, the core leader will manage and coordinate with core personnel all aspects of the transplantation. In addition, core personnel will perform transplants into NOD/SCID mice, harvest and provide tissues from NOD/SCID experiments to investigators of this program project for analysis. This will also be coordinated with the "Cell Purification and Analysis Laboratory (Core A)." The core leader will also provide assistance with experimental design and interpretation of data generated in NOD/SCID xeriograft transplantation experiments. The shared Core A will provide participants in the program project with costeffective and high quality services for their research. These studies will provide new information regarding hematopoietic stem-cell function in vivo that is applicable to the future development of gene therapy trials.