The Role of Copper in Pediatric Liver Disease
铜在小儿肝病中的作用
基本信息
- 批准号:7741190
- 负责人:
- 金额:$ 12.68万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2000
- 资助国家:美国
- 起止时间:2000-12-01 至 2009-11-30
- 项目状态:已结题
- 来源:
- 关键词:ATP phosphohydrolaseAntioxidantsBase SequenceBiliaryBindingBiochemicalBiochemistryBiological AssayCell RespirationCellsCellular biologyCeruloplasminChildChildhoodChimeric ProteinsComplementary DNAComplexConditionCopperDiseaseEventExcretory functionFunctional disorderGenesGlutathione S-TransferaseHepaticHepatobiliaryHepatocyteHepatolenticular DegenerationHomeostasisHomologous GeneHumanIn VitroInjuryIronLeadLiverLiver diseasesMass Spectrum AnalysisMediatingMembraneMetabolismMetalsMissense MutationMolecular ChaperonesMusMutationNumbersOrganPathway interactionsPatientsPeptide Sequence DeterminationProcessProteinsRoleSite-Directed MutagenesisStructureTrace ElementsTransgenic MiceTransgenic OrganismsTranslatingbasecDNA Libraryexpression cloninginsightmutantnovel therapeuticspreventprotein expressiontraffickingtwo-dimensionaluptake
项目摘要
DESCRIPTION (provided by applicant): Copper is an essential trace element with a critical role in the biochemistry of cellular respiration, antioxidant defense and iron homeostasis. The liver is the central organ of copper homeostasis in humans and the long-term objective of these studies is to define the role of copper in pediatric liver disease. While previous studies have revealed a central role for the copper chaperone Atox1 and the Wilson disease ATPase (ATP7b) in hepatic copper metabolism, the mechanisms within the hepatocyte secretory pathway that lead to excretion of this metal at the canalicular membrane remain poorly understood. Most recently, a direct interaction has been demonstrated between ATP7b and Murr1, the human homologue of the Bedlington terrier copper toxicosis gene product, providing biochemical evidence in support of a role for Mutt1 in the hepatobiliary pathophysiology of copper excretion. The specific aims of this proposal are intended to elucidate the role of Murr1 and ATP7b in this process. Structure/function studies will be accomplished using site-directed mutagenesis to delineate the biochemical details of Murr1 interaction with ATP7b. The pathophysiology of hepatic copper toxicosis in patients with missense mutations in Murr1 will be elucidated by examining copper homeostasis and ATP7b function in mice transgenic for these mutations as well as mice with a targeted germline deletion of the Murr1 gene. The functional role of Murr1 in copper excretion will be determined by isolation and characterization of specific interacting proteins present in Murr1 heterooligomeric complexes formed upon interaction with ATP7b. Finally, the role of the carboxyl terminus of ATP7b in copper-mediated trafficking and excretion in hepatocytes will be defined using in vitro expression cloning to characterize the proteins interacting with this domain. Taken together the results of these studies will permit new insights into the hepatobiliary pathophysiology of excretory events within the liver and may allow for novel therapeutic approaches to prevent or ameliorate hepatic injury in a number of pediatric liver diseases.
描述(由申请人提供):铜是一种必需的微量元素,在细胞呼吸,抗氧化防御和铁稳态的生物化学中起着关键作用。肝脏是人体铜稳态的中心器官,这些研究的长期目标是确定铜在儿童肝脏疾病中的作用。虽然先前的研究已经揭示了铜伴侣Atox1和Wilson病atp酶(ATP7b)在肝铜代谢中的核心作用,但肝细胞分泌途径内导致这种金属在管膜排泄的机制仍然知之甚少。最近,ATP7b和Murr1 (Bedlington梗铜中毒基因产物的人类同源物)之间的直接相互作用已被证实,为Mutt1在铜排泄的肝胆病理生理中的作用提供了生化证据。本提案的具体目的是阐明Murr1和ATP7b在这一过程中的作用。结构/功能研究将使用定点诱变技术来描述Murr1与ATP7b相互作用的生化细节。通过检测铜稳态和ATP7b功能,将阐明Murr1错义突变患者肝铜中毒的病理生理机制,这些突变转基因小鼠和Murr1基因靶向种系缺失小鼠的铜稳态和ATP7b功能。Murr1在铜排泄中的功能作用将通过与ATP7b相互作用形成的Murr1异聚物复合物中存在的特异性相互作用蛋白的分离和表征来确定。最后,ATP7b的羧基末端在铜介导的肝细胞运输和排泄中的作用将通过体外表达克隆来确定与该结构域相互作用的蛋白质。综上所述,这些研究的结果将为肝脏内排泄事件的肝胆病理生理学提供新的见解,并可能为预防或改善许多儿童肝脏疾病的肝损伤提供新的治疗方法。
项目成果
期刊论文数量(0)
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Jonathan David Gitlin其他文献
Jonathan David Gitlin的其他文献
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{{ truncateString('Jonathan David Gitlin', 18)}}的其他基金
MECHANISMS OF GROWTH AND THE OVERGROWTH SYNDROMES
生长机制和过度生长综合症
- 批准号:
6737561 - 财政年份:2001
- 资助金额:
$ 12.68万 - 项目类别:
MECHANISMS OF GROWTH AND THE OVERGROWTH SYNDROMES
生长机制和过度生长综合症
- 批准号:
6895276 - 财政年份:2001
- 资助金额:
$ 12.68万 - 项目类别:
MECHANISMS OF GROWTH AND THE OVERGROWTH SYNDROMES
生长机制和过度生长综合症
- 批准号:
6536286 - 财政年份:2001
- 资助金额:
$ 12.68万 - 项目类别:
MECHANISMS OF GROWTH AND THE OVERGROWTH SYNDROMES
生长机制和过度生长综合症
- 批准号:
6288561 - 财政年份:2001
- 资助金额:
$ 12.68万 - 项目类别:
MECHANISMS OF GROWTH AND THE OVERGROWTH SYNDROMES
生长机制和过度生长综合症
- 批准号:
6638003 - 财政年份:2001
- 资助金额:
$ 12.68万 - 项目类别:
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