Role of C1C5 in Endocytosis and NHE3 Trafficking

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Dent's disease, is a disorder that is characterized by low molecular weight proteinuria, aminoaciduria, glycosuria, polyuria, hyperphosphaturia (Fanconi syndrome) as well as hypercalciuria, nephrocalcinosis, nephrolithiasis. Dent's disease has been established to be caused by mutations of the CICN-5 gene, the product of which is CIC-5 a voltage-gated chloride channel expressed in highest abundance in renal tubules of the proximal nephron. Our preliminary studies in a CICN-5 knockout mouse model, shows that it recapitulates the features of Dent's disease including hypercalciuria, aminoaciduria, glycosuria, low molecular weight proteinuria, renal calcifications, and renal failure. THE PRPOSED STUDY WILL HELP DEFINE THE UNDERLYING MOLECULAR BASIS OF THESE REABSORPTION DEFECTS. Specific aim 1: How do other CIC channels or CIC-5 exchange regulate apical endocytosis in proximal tubule cells? 1 A. Do CIC-3 and/or CIC-4 contribute to the residual acidification and apical endocytosis in the ko cells? 18. Does the over-expression of CIC-3 and CIC-4 restore albumin uptake in CIC-5 ko cells? Does the chloride/proton exchanger activity of CIC-5 in ko cells restore normal albumin uptake? Specific Aim 2: What is the relationship between loss of CIC-5 and the downregulation of megalin in the ko proximal tubule cells? 2A. Is the half-life of megalin shorter in ko versus wt cells? 2B. How is the recycling behavior of megalin altered in ko versus wt cells? 2C. Does endosomal pH, or lack of CIC-5, regulate megalin dynamics? Specific Aim 3. How does the loss of CIC-5 lead to changes in the distribution/activity of NHE3 and other apical transporters? 3A. Is NHE3 activity decreased in the proximal tubule, ileum or jejunum of ko mice? 3B. What are the steady state levels and dynamics of NHE3 in ko proximal tubules cells? 3C. What are the steady state levels and dynamics of NaPi-lla in ko proximal tubule cells? 3D, Does endosomal pH, or lack of CIC-5, regulate NHE3 or NaPi-lla dynamics? The long term goal of this research is to understand how molecular events cause the phenotypical outcomes of Dent's disease.