CORE--CONUS VENOMS RESOURCE

核心--芋螺毒液资源

• 批准号: 7551096
• 负责人: LOURDES J CRUZ
• 金额: $ 19.04万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7551096
• 关键词: Biological Assay; Class; Cloning; Collection; Complementary DNA; Conotoxin; Conus Venom; Conus genus; Core Facility; Country; Databases; Duct (organ) structure; Enzymatic Biochemistry; Fishes; Fractionation; Grant; Hepatopancreas; Institutes; Marines; Molecular; Operative Surgical Procedures; Peptides; Philippines; Post-Translational Protein Processing; Preparation; Process; Resources; Science; Source; Specimen; System; Tissues; Universities; Utah; Venoms; analog; cDNA Library; synthetic peptide

Description. Since its establishment in the first grant period, the Venom Resource Core has expanded to a collection of over 100 Conus species, and the facility has developed to a highly efficient operation for identification of specific venom components. Collection of Conus specimens from several countries will continue, but most of them will be obtained from various localities of the Philippines, the richest source of Conus species; about 200 of the 500 known species are found in the Philippines. In most cases, venom is processed in the Marine Science Institute of the University of the Philippines and transported to the University of Utah where a system for rapid assay, fractionation and purification has been developed; the collection of whole venom ducts and tissues (particularly hepatopancreas) has been greatly expanded. The latter are the starting point for molecular analyses. The Core will concentrate on two types of collection activities: (1) Increasing the amount of venom for new classes of fish-hunting species such as Conus bocki, C. bullatus, C. kinoshitai, C. parius, C. flavus, C. strioIatus, C. achatinus, C. spectrum and C. cinereus. (2) Obtaining tissues or preserved specimens of the remaining fish-hunting Conus species, including some that are either very rare of that come from inaccessible localities. Among these are Conus cervus, C. dusaveli, C. proximus, C. moIucensis, C. collisus and C. subulatus. Purified natural Conus peptides, synthetic peptides, derivatives and analogs will also be maintained by the core facility. The collection of venom ducts and other tissues from selected Conus species will be used for preparation of cDNA libraries which will be needed to rapidly expand conotoxin sequence databases through cDNA cloning. Furthermore, many thousands of venom ducts will be collected as part of our increasing efforts in the enzymology of post-translational modification.

描述。自第一个赠款时期建立以来，毒液资源核心拥有 扩展到100多种圆锥物种的集合，该设施已发展为高效的操作，以识别特定的毒液成分。将继续收集来自几个国家的圆锥标本，但其中大多数将从菲律宾的各个地区获得，这是最富有的圆锥形物种来源。在菲律宾发现了500种已知物种中的大约200种。 在大多数情况下，毒液是在菲律宾大学海洋科学研究所加工的，并运送到犹他大学，那里已经开发了快速测定，分馏和纯化系统；整个毒管和组织（尤其是肝癌）的收集已大大扩展。后者是分子分析的起点。核心将集中在两种类型的收集活动上：（1）增加新的鱼类狩猎物种的毒液量，例如Conus Bocki，C。Bullatus，C。Kinoshitai，C。Parius，C。Flavus，C。Flavus，C。strioiatus，C。achatinus，C。achatinus，C。c。c。c。pepsprum和C. cinereus。 （2）获取其余鱼类圆锥形物种的组织或保留的标本，其中包括一些非常罕见的地方来自无法接近的地方。 其中包括Conus cervus，C。dusaveli，C。proximus，C。moiucensis，C。collisus和C. subulatus。 纯化的天然圆锥肽，合成肽，衍生物和类似物也将由核心设施维持。从选定的圆锥形物种中收集的毒液管和其他组织将用于制备cDNA库，这些cDNA文库将需要通过cDNA克隆快速扩展结合毒素序列数据库。 此外，将收集数千种毒管，作为我们在翻译后修饰的酶学中日益增长的努力的一部分。