The Respiratory Eff. Study of Aerosolized Red Tide Toxins in Humans

呼吸效应。

• 批准号: 7479617
• 负责人: LORA Elderkin FLEMING
• 金额: $ 33.23万
• 依托单位: UNIVERSITY OF NORTH CAROLINA WILMINGTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7479617
• 关键词: Acute; Aerosols; Animal Model; Asthma; Biological Assay; Centers for Disease Control and Prevention (U.S.); Chronic; Data; Development; Dose; Environment; Environmental Exposure; Evaluation; Exhalation; Exposure to; Florida; Follow-Up Studies; Grant; Gymnodinium breve toxin; Health; Hospitalization; Hour; Human; Inflammatory; Life; Logistics; Measurable; Measurement; Measures; Mediator of activation protein; Medical; Methods; Monitor; Nose; Occupational; Pharmaceutical Preparations; Physiological; Population; Public Health; Reporting; Research Personnel; Research Project Grants; Respiratory physiology; Sampling; Science; Severities; Spirometry; Statistically Significant; Swab; Symptoms; Testing; Time; Toxin; Visit; Work; aerosolized; animal data; base; brevetoxin; cohort; cytokine; day; exposed human population; follow-up; improved; indexing; programs; red tide; respiratory; response

Over the past 3 years, the Research Project 4 (RP4) Investigators have assembled a cohort of asthmatics and studied their respiratory responses to Florida red tide by comparing exposure and nonexposure periods. We have demonstrated statistically significant changes in inflammatory markers, respiratory function and reported symptoms in asthmatics with only 1 hour of acute beach exposure; no significant changes were observed during similar non-exposure periods. Parallel to these studies, the Investigators have assisted the Centers for Disease Control and Prevention (CDC) and the Florida Dept of Health (FDOH) in assembling and evaluating an occupational cohort. This represents the first time that possible health effects from exposure to aerosolized Florida red tide toxins have been evaluated epidemiologically in human populations. Over the next 5 years, working with the Toxin Probes and Assays Core, the Field Logistics and Development Core, and Project by Cheng, we propose to continue studying the exposures and health effects for humans due to aerosol exposures to Florida Red Tide, using new measures of exposure and physiologic effects. Specifically, we will annually collect pre- and post-exposure health effects and environmental exposure data at a time when a Florida red tide occurs and at a time with no Florida red tide for 1 hour of beach exposure. In addition, the investigators will annually conduct a 5 day Follow-up Study directly following the 1 hour Acute Exposure Study to collect data on persistent and delayed health effects from the acute exposure, as well as from possible repetitive exposures to Florida red tides toxins over 5 days. Additional data will be collected every 6 months to evaluate the severity and controllability of the asthmatics in the prior 6 months. The data from these annual studies (exposed and unexposed) will be evaluated longitudinally to explore possible chronic effects of varying Florida red tide toxin levels on the health of asthmatics, as well as subpopulations among the asthmatics. Based on the results of the animals models ( Projects by Benson and Abraham) and our epidemiologic work, we will use the following measurable physiologic effects: reported symptoms and measures of asthma controllability, spirometry, nasal swabs for inflammatory mediators, and exhaled condensates for acidification and selected cytokines, as well as the exposure measures of environmental and personal monitoring and nasal swabs for brevetoxins. The possible association between the uncontrollability of asthma associated with Florida red tide toxin exposure over time will be evaluated. In addition, the possible dose response relationship will be explored between long term exposure to Florida red tide toxins and their effects on measurable physiologic effects in asthmatics by evaluating coastal vs inland residents. Over the proposed 5 years, the project investigators will also take advantage of advancements in the science made by the other Projects to refine methods for measuring human exposures and physiologic effects from this environmental occurrence. The data from these studies will provide valuable medical and public health information concerning acute, subacute and chronic respiratory effects of aerosolized red tide toxin exposures annually in asthmatics over a 5 year period.

在过去的3年中，研究项目4（RP4）调查人员组建了一群 哮喘患者并通过比较暴露和不暴露研究了他们对佛罗里达红潮的呼吸反应 时期。我们已经证明了炎症标记的统计学显着变化， 呼吸功能和报道的哮喘病症状只有1小时的急性海滩暴露；不 在类似的非暴露期间观察到显着变化。与这些研究平行， 研究人员已协助疾病控制与预防中心（CDC）和佛罗里达部门 卫生（FDOH）组装和评估职业队列。这代表了第一次 已经评估了从暴露于佛罗里达红潮毒素的毒素的健康影响 在人类人群中流行病学。 在接下来的5年中，使用毒素探针和测定核心，现场物流和开发核心以及Cheng的项目，我们建议 由于气溶胶暴露于佛罗里达州，继续研究人类的暴露和健康影响 红潮，采用新的暴露和生理效果的措施。具体来说，我们每年都会收集 在佛罗里达红潮时，暴露前后的健康效果和环境暴露数据 发生并且一次没有佛罗里达红潮，持续1小时的海滩曝光。此外，调查人员 1小时急性暴露研究后，每年将直接进行为期5天的随访研究 从急性暴露以及可能的 在5天内，重复暴露于佛罗里达红潮汐毒素。每6个将收集其他数据 几个月来评估前6个月哮喘患者的严重性和可控性。来自 这些年度研究（暴露和暴露）将进行纵向评估，以探索可能 佛罗里达红潮毒素水平不同的慢性影响对哮喘患者的健康以及 哮喘患者中的亚群。 根据动物模型（Benson和Abraham的项目）和我们的流行病学工作的结果，我们 将使用以下可测量的生理效应：报告的症状和哮喘测量值 可控性，肺活量测量，鼻拭子，用于炎症介质和呼出的冷凝物 酸化和选定的细胞因子以及环境和个人的暴露措施 监测和鼻拭子，以供brevetoxins。在 随着时间的推移，将评估与佛罗里达红潮毒素暴露有关的哮喘。另外， 长期接触佛罗里达红潮将探索可能的剂量反应关系 通过评估沿海与内陆，毒素及其对哮喘患者中可测量的生理影响的影响 居民。在提议的5年中，项目调查人员还将利用 其他项目为完善人类暴露和 这种环境发生的生理效应。这些研究的数据将提供 有关急性，亚急性和慢性呼吸道的宝贵医疗和公共卫生信息 在5年的哮喘患者中，每年在哮喘病中暴露了雾化的红潮毒素的影响。