ATOMIC FORCE MICROSCOPE

原子力显微镜

• 批准号: 7394176
• 负责人: Mark Paul Goldberg
• 金额: $ 27.5万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7394176
• 关键词: Alzheimer' s Disease; Atomic Force Microscopy; Bacteriology; Biomedical Engineering; Brain Neoplasms; Chemistry; Condition; Contract Services; Core Facility; Data Analyses; Equipment; Financial Support; Fluorescence Microscopy; Funding; Funding Applicant; Future; Grant; Head; Human Resources; Microfluidics; Microscope; Microscopy; Minor; Molecular Analysis; Nanotechnology; Nerve Degeneration; Neurology; Optics; Parkinson Disease; Publishing; Research; Research Personnel; Scanning; Scanning Probe Microscopes; Schedule; Technical Expertise; Training; Tumor Biology; United States National Institutes of Health; Universities; Update; Washington; Yin; cell motility; cellular imaging; digital; experience; fluorescence imaging; instrument; instrumentation; medical schools; nervous system disorder; protein aggregation; protocol development; vibration

DESCRIPTION (provided by applicant): Funds are requested to establish an integrated Atomic Force Microscopy (AFM) facility for NIH-funded research at Washington University School of Medicine. There is a pressing need for updated AFM capabilities as there is currently no AFM on the School of Medicine campus, and AFM instruments elsewhere at Washington University are fully utilized and technically outdated. The proposal will support 22 NIH grant projects of 6 Major Users (with published AFM experience), and 7 Minor Users. Planned AFM applications include cell motility, bacteriology, brain tumor biology, microfluidics, and nanotechnology. Because an important research focus is protein aggregation in neurodegenerative conditions such as Alzheimer's and Parkinson's diseases, the AFM will be acquired by the Hope Center for Neurological Disorders, an interdepartmental research unit administered within the Department of Neurology. The Hope Center will provide financial support for renovation, service contracts, and personnel. Installation within an established microscopy core facility will assist in user training, protocol development, scheduling, data analysis and dissemination. A highly experienced AFM technician will train and assist users. Strong technical expertise for the AFM facility is provided by co-investigators Frank Yin (Department of Biomedical Engineering) and Karen Wooley (Department of Chemistry), major users who operate current Washington University AFM labs. The requested instrumentation includes a Veeco (formerly Digital) Nanoscope V control station operating two scanning probe heads: PicoForce MultiMode (optimized for atomic scale molecular analysis), and BioScope II (for cellular imaging and simultaneous AFM/fluorescence imaging on a Zeiss Axiovert optical microscope), together with required equipment for vibration isolation and environmental control. The proposed configuration will allow optimal integration of optical microscopy and other AFM facilities at Washington University, with potential for significant future expansion as user needs increase.

描述（由申请人提供）：资金被要求建立一个综合原子力显微镜（AFM）设施NIH资助的研究在华盛顿大学医学院。迫切需要更新原子力显微镜功能，因为目前医学院校园内没有原子力显微镜，而华盛顿大学其他地方的原子力显微镜仪器已得到充分利用，但技术上已经过时。该提案将支持22个NIH资助项目，包括6个主要用户（已发表AFM经验）和7个次要用户。计划中的AFM应用包括细胞运动、细菌学、脑肿瘤生物学、微流体学和纳米技术。由于一个重要的研究重点是蛋白质聚集在神经退行性疾病，如阿尔茨海默氏症和帕金森氏症，原子力显微镜将被收购的希望中心神经疾病，跨部门的研究单位管理内的神经科。希望中心将提供翻新、服务合同和人员方面的资金支持。在现有的显微镜核心设施内进行安装将有助于用户培训、方案制定、时间安排、数据分析和传播。经验丰富的AFM技术人员将培训和协助用户。强大的AFM设施的技术专长是由共同研究员弗兰克尹（生物医学工程系）和凯伦伍利（化学系），主要用户谁经营目前华盛顿大学AFM实验室提供。 要求的仪器包括一个Veeco（以前的数字）Nanoscope V控制站，操作两个扫描探头：PicoForce MultiMode（优化原子尺度分子分析）和BioScope II（用于细胞成像和同步AFM/荧光成像的蔡司Axiovert光学显微镜），以及所需的振动隔离和环境控制设备。拟议的配置将允许光学显微镜和其他原子力显微镜设施在华盛顿大学的最佳集成，随着用户需求的增加，具有显着的未来扩展的潜力。