ATOMIC FORCE MICROSCOPE

原子力显微镜

• 批准号: 7394176
• 负责人: Mark Paul Goldberg
• 金额: $ 27.5万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7394176
• 关键词: Alzheimer' s Disease; Atomic Force Microscopy; Bacteriology; Biomedical Engineering; Brain Neoplasms; Chemistry; Condition; Contract Services; Core Facility; Data Analyses; Equipment; Financial Support; Fluorescence Microscopy; Funding; Funding Applicant; Future; Grant; Head; Human Resources; Microfluidics; Microscope; Microscopy; Minor; Molecular Analysis; Nanotechnology; Nerve Degeneration; Neurology; Optics; Parkinson Disease; Publishing; Research; Research Personnel; Scanning; Scanning Probe Microscopes; Schedule; Technical Expertise; Training; Tumor Biology; United States National Institutes of Health; Universities; Update; Washington; Yin; cell motility; cellular imaging; digital; experience; fluorescence imaging; instrument; instrumentation; medical schools; nervous system disorder; protein aggregation; protocol development; vibration

DESCRIPTION (provided by applicant): Funds are requested to establish an integrated Atomic Force Microscopy (AFM) facility for NIH-funded research at Washington University School of Medicine. There is a pressing need for updated AFM capabilities as there is currently no AFM on the School of Medicine campus, and AFM instruments elsewhere at Washington University are fully utilized and technically outdated. The proposal will support 22 NIH grant projects of 6 Major Users (with published AFM experience), and 7 Minor Users. Planned AFM applications include cell motility, bacteriology, brain tumor biology, microfluidics, and nanotechnology. Because an important research focus is protein aggregation in neurodegenerative conditions such as Alzheimer's and Parkinson's diseases, the AFM will be acquired by the Hope Center for Neurological Disorders, an interdepartmental research unit administered within the Department of Neurology. The Hope Center will provide financial support for renovation, service contracts, and personnel. Installation within an established microscopy core facility will assist in user training, protocol development, scheduling, data analysis and dissemination. A highly experienced AFM technician will train and assist users. Strong technical expertise for the AFM facility is provided by co-investigators Frank Yin (Department of Biomedical Engineering) and Karen Wooley (Department of Chemistry), major users who operate current Washington University AFM labs. The requested instrumentation includes a Veeco (formerly Digital) Nanoscope V control station operating two scanning probe heads: PicoForce MultiMode (optimized for atomic scale molecular analysis), and BioScope II (for cellular imaging and simultaneous AFM/fluorescence imaging on a Zeiss Axiovert optical microscope), together with required equipment for vibration isolation and environmental control. The proposed configuration will allow optimal integration of optical microscopy and other AFM facilities at Washington University, with potential for significant future expansion as user needs increase.

描述（由申请人提供）：请求资金在华盛顿大学医学院建立一个综合原子力显微镜 (AFM) 设施，用于 NIH 资助的研究。由于目前医学院校园没有 AFM，而华盛顿大学其他地方的 AFM 仪器已充分利用且技术过时，因此迫切需要更新 AFM 功能。该提案将支持 6 个主要用户（已发表 AFM 经验）和 7 个次要用户的 22 个 NIH 资助项目。计划的 AFM 应用包括细胞运动、细菌学、脑肿瘤生物学、微流体和纳米技术。由于一个重要的研究重点是阿尔茨海默病和帕金森病等神经退行性疾病中的蛋白质聚集，因此 AFM 将被希望神经疾病中心收购，该中心是神经病学系管理的跨部门研究单位。希望中心将为翻修、服务合同和人员提供财政支持。安装在已建立的显微镜核心设施内将有助于用户培训、方案开发、调度、数据分析和传播。经验丰富的 AFM 技术人员将培训和协助用户。联合研究员 Frank Yin（生物医学工程系）和 Karen Wooley（化学系）为 AFM 设施提供了强大的技术专业知识，他们是当前华盛顿大学 AFM 实验室的主要用户。 所需的仪器包括一个 Veeco（以前称为 Digital）Nanscope V 控制站，操作两个扫描探头：PicoForce MultiMode（针对原子级分子分析进行了优化）和 BioScope II（用于在 Zeiss Axiovert 光学显微镜上进行细胞成像和同时 AFM/荧光成像），以及所需的隔振和环境控制设备。拟议的配置将允许华盛顿大学光学显微镜和其他 AFM 设施的最佳集成，随着用户需求的增加，未来具有显着扩展的潜力。