ATOMIC FORCE MICROSCOPE

描述（由申请人提供）：资金被要求建立一个综合原子力显微镜（AFM）设施NIH资助的研究在华盛顿大学医学院。有一个更新的AFM能力的迫切需要，因为目前没有AFM的医学院校园，和AFM仪器在其他地方在华盛顿大学充分利用和技术过时。该提案将支持22个NIH资助项目，包括6个主要用户（已发表AFM经验）和7个次要用户。计划中的AFM应用包括细胞运动、细菌学、脑肿瘤生物学、微流体学和纳米技术。由于一个重要的研究重点是蛋白质聚集在神经退行性疾病，如阿尔茨海默氏症和帕金森氏症，原子力显微镜将被收购的希望中心神经疾病，跨部门的研究单位管理内的神经科。希望中心将提供翻新、服务合同和人员方面的资金支持。在现有的显微镜核心设施内进行安装将有助于用户培训、方案制定、时间安排、数据分析和传播。经验丰富的AFM技术人员将培训和协助用户。强大的AFM设施的技术专长是由共同研究员弗兰克尹（生物医学工程系）和凯伦伍利（化学系），主要用户谁经营目前华盛顿大学AFM实验室提供。 所要求的仪器包括一个Veeco（以前的数字）Nanoscope V控制站，操作两个扫描探头：PicoForce MultiMode（针对原子尺度分子分析进行了优化）和BioScope II（用于在Zeiss Axiovert光学显微镜上进行细胞成像和同步AFM/荧光成像），以及用于振动隔离和环境控制的所需设备。拟议的配置将允许光学显微镜和其他原子力显微镜设施在华盛顿大学的最佳集成，随着用户需求的增加，具有显着的未来扩展的潜力。

DESCRIPTION (provided by applicant): Funds are requested to establish an integrated Atomic Force Microscopy (AFM) facility for NIH-funded research at Washington University School of Medicine. There is a pressing need for updated AFM capabilities as there is currently no AFM on the School of Medicine campus, and AFM instruments elsewhere at Washington University are fully utilized and technically outdated. The proposal will support 22 NIH grant projects of 6 Major Users (with published AFM experience), and 7 Minor Users. Planned AFM applications include cell motility, bacteriology, brain tumor biology, microfluidics, and nanotechnology. Because an important research focus is protein aggregation in neurodegenerative conditions such as Alzheimer's and Parkinson's diseases, the AFM will be acquired by the Hope Center for Neurological Disorders, an interdepartmental research unit administered within the Department of Neurology. The Hope Center will provide financial support for renovation, service contracts, and personnel. Installation within an established microscopy core facility will assist in user training, protocol development, scheduling, data analysis and dissemination. A highly experienced AFM technician will train and assist users. Strong technical expertise for the AFM facility is provided by co-investigators Frank Yin (Department of Biomedical Engineering) and Karen Wooley (Department of Chemistry), major users who operate current Washington University AFM labs. The requested instrumentation includes a Veeco (formerly Digital) Nanoscope V control station operating two scanning probe heads: PicoForce MultiMode (optimized for atomic scale molecular analysis), and BioScope II (for cellular imaging and simultaneous AFM/fluorescence imaging on a Zeiss Axiovert optical microscope), together with required equipment for vibration isolation and environmental control. The proposed configuration will allow optimal integration of optical microscopy and other AFM facilities at Washington University, with potential for significant future expansion as user needs increase.