NCRR Confocal Proposal

描述（由申请人提供）：我们建议使用电动XY载物台和SIM扫描仪获取Olympus FV 1000光谱共焦。该仪器将保存在犹他州大学医学院细胞成像核心设施。购买FV 1000共聚焦和配件将扩大犹他州大学现有成像技术的范围，并取代老化的共聚焦，这是我们设施的重要组成部分。将被替换的仪器在细胞成像核心设施中有着长期的大量使用历史，并使用医学院提供的资源进行了维护和升级。本建议书详细说明了主要用户确定的各种成像项目如何得到所需仪器特有的功能和附件组合的支持。拟议的仪器将确保细胞成像设施为这些项目提供支持的能力的连续性，拟议的附件将扩大整个校园可用的成像工具阵列。被确定为主要用户的研究人员在这项补助金已被选定为他们的要求，先进的共聚焦成像，NIH的资金，以及未来的潜在利益，从收购另一个高端共聚焦，以取代已被最大限度地使用的仪器。Cell Imaging Core机构的主要用户和其他人的共聚焦使用历史为我们的断言提供了定量支持，即新仪器将被最大限度地使用，并且随着仪器老化，通过适当的维护、培训和适当的升级，其使用寿命将延长。简介：我们建议使用电动XY载物台和SIM扫描仪获取Olympus FV 1000光谱共焦。该仪器将保存在犹他州大学医学院细胞成像核心设施。购买FV 1000共聚焦和配件将扩大犹他州大学现有成像技术的范围，并取代老化的共聚焦，这是我们设施的重要组成部分。细胞成像核心设施的使命是作为当地的设备、技术专长和培训资源，以最大限度地发挥犹他州大学现有成像技术和设备的潜力。该设施有三名全职工作人员，包括主任（博士），一个程序员和一个显微镜技术员工作人员的唯一职责是维护仪器，开发新的成像和图像处理技术，并培训用户。细胞成像设施由核心设施部门监督，这是一个高度组织化的基础设施，旨在确保成像和其他技术投资的科学影响最大化，并保持设备和资源的自由和公平获取。将被替换的仪器在细胞成像核心设施中有着长期的大量使用历史，并使用医学院提供的资源进行了维护和升级。拟议的仪器的主要用户代表了广泛的研究兴趣，从发育生物学的药物输送系统的调查。这些用户中的每一个都有一个共同点，那就是NIH的资助历史悠久，并且需要先进的成像技术。拟议的仪器将确保细胞成像设施为这些项目提供支持的能力的连续性，拟议的附件将扩大整个校园可用的成像工具阵列。

DESCRIPTION (provided by applicant): We propose to acquire an Olympus FV1000 spectral confocal with a motorized XY stage and a SIM scanner. This instrument will be maintained at the University of Utah School of Medicine Cell Imaging Core facility. Acquisition of the FV1000 confocal and accessories will expand the range of imaging technology available at the University of Utah and replace an aging confocal that is a vital component of our facility. The instrument that will be replaced has a consistent history of heavy usage at the Cell Imaging Core facility, and has been maintained and upgraded using resources provided by the School of Medicine. This proposal provides details of how the diverse array of imaging projects identified by the Major Users are supported by the specific combination of features and accessories found only in the requested instrument. The proposed instrument will ensure continuity in the ability of the Cell Imaging facility to provide support to these projects, and the proposed accessories will expand the array of imaging tools available campus-wide. The investigators identified as Major Users in this grant have been selected for their requirement for advanced confocal imaging, significant degree of NIH funding, and future potential benefit from acquisition of another high-end confocal to replace an instrument that has been maximally used. The confocal usage history of the Major Users and others at the Cell Imaging Core facility provide quantitative support for our assertion that a new instrument would be used maximally, and that its useful lifetime would be extended by proper maintenance, training and appropriate upgrades as the instrument ages.1. Introduction: We propose to acquire an Olympus FV1000 spectral confocal with a motorized XY stage and a SIM scanner. This instrument will be maintained at the University of Utah School of Medicine Cell Imaging Core facility. Acquisition of the FV1000 confocal and accessories will expand the range of imaging technology available at the University of Utah and replace an aging confocal that is a vital component of our facility. The mission of the Cell Imaging Core facility is to serve as a local resource of equipment, technical expertise and training in order to maximize the potential of imaging technology and equipment available at the University of Utah. The facility has three full-time staff including the director (Ph.D.), a programmer, and a microscopy technician. The sole responsibility of the staff is to maintain the instruments develop new imaging and image processing techniques, and train users. The Cell Imaging facility is overseen by the Core Facility department, which is a highly organized infrastructure designed to ensure that the scientific impact from investment in imaging and other technology is maximized, and that free and fair access to equipment and resources is maintained. The instrument that will be replaced has a consistent history of heavy usage at the Cell Imaging Core facility, and has been maintained and upgraded using resources provided by the School of Medicine. The Major Users of the proposed instrument represent a broad range of research interests from developmental biology to investigation of drug delivery systems. Each of these users has in common a strong history of NIH funding and a need for advanced imaging technology. The proposed instrument will ensure continuity in the ability of the Cell Imaging facility to provide support to these projects, and the proposed accessories will expand the array of imaging tools available campus-wide.