Circular Dichroism Spectrometer

项目描述（由申请人提供）：本项目的目标是购买一台最先进的高灵敏度圆二色谱仪（JASCO J-815），并将其提供给整个爱因斯坦医学院科学界。该仪器将取代已有18年历史的CD光谱仪，该光谱仪灵敏度低得多，可靠性差，功能有限。新JASCO仪器的功能将远远超出目前校园内可用的任何仪器，因为它将结合圆二色性，光学吸收和荧光光谱，可以在平衡，滴定，温度控制和停止流动条件下进行。在美国国立卫生研究院的支持下，有几个用户对该仪器有基本需求，以实现其项目的目标。一旦进入校园，预计科学界的许多其他成员也将利用该仪器来开展他们的项目。该仪器的可用性将通过电子邮件宣布，其功能的完整描述以及调度信息和技术文件将在一个新的网站上永久提供。

DESCRIPTION (provided by applicant): The goal of this project is to purchase a state-of-the-art high sensitivity Circular Dichroism Spectrometer (JASCO J-815) and to make it available to the entire Albert Einstein College of Medicine scientific community. This instrument will replace an 18-year old CD spectrometer that has much lower sensitivity, poor reliability and limited functions. The capabilities of the new JASCO instrument will extend well beyond those of any instrument currently available on the campus, as it will combine circular dichroism, optical absorption and fluorescence spectroscopies that can be made under equilibrium, titration, temperature-controlled and stopped-flow conditions. There are several users, with National Institutes of Health support, who have an essential need of the instrumentation to fulfill the aims of their projects. Once on campus it is anticipated that many other members of the scientific community will take advantage of this instrumentation to carry out their projects as well. The availability of the instrument will be announced by e-mail and a full description of its capabilities, along with scheduling information and technical documents, will be permanently available on a new web site. The planned projects with the instrumentation span a wide spectrum of biomedical areas including mechanistic studies of several enzymatic processes in physiologically important enzymes, studies of proteins that are believed to be involved in conferring drug and/or host resistance in the human pathogens, M. tuberculosis and C. jejuni, characterization of blood substitutes, which are in clinical trials, design and characterization of transition state inhibitors, which are in clinical trials for leukemia T-cell autoimmune disease, and determination of the fiber formation processes in 1-synuclein, the principle component of the neural tangle aggregates in Parkinson's disease. This instrumentation will be very relevant to public health in both the near and long term. It will immediately yield better understanding and advances in the development of blood substitutes and anti-cancer drugs. In the longer term, it will give new information on critical proteins from human pathogens, which may allow for new anti-microbial strategies, and finally, new mechanistic information on several human enzymes will be valuable for future clinical studies including the understanding of diseases and the development of new pharmaceutical agents.