XCT Research SA Plus pQCT Scanner

描述（由申请人提供）： 该应用程序的目标是为耶鲁医学院的研究人员提供一种对骨生物学感兴趣的仪器，以必要的方式推动研究。该仪器是一种外周定量计算机断层扫描（pQCT），可提供有关小型活体动物（如小鼠和大鼠）骨小梁和皮质骨密度的精确和快速定量信息。耶鲁大学是一大批对骨生物学感兴趣的研究人员的家园，他们越来越频繁地使用大鼠和基因工程小鼠在体内翻译他们的方法。这些研究人员要么是在骨生物学领域建立良好的，要么是新的，并与耶鲁大学医学院的几个部门，包括细胞生物学，免疫生物学，医学，整形外科，病理学，药理学，比较医学和整形外科部门。然而，尽管耶鲁大学拥有大量的骨生物学家，但它并没有配备最先进的设备来确定用作实验模型的小动物的骨的数量和质量。因此，本申请的具体目的是获得pQCT，以确定用骨合成代谢或抗分解代谢药物/激素治疗的活动物的骨小梁和皮质骨密度，以评估治疗的功效。pQCT可以测定经基因工程改造以过度表达或缺乏在骨发育、修复或稳态中起核心作用的特定基因的活小鼠中的小梁和皮质骨密度。pQCT还可以确定切除骨中的骨密度。本申请的主要研究者和关键人员共同致力于进一步了解骨生物学，共同目标是预防和修复骨丢失，并治疗骨发育和/或质量改变的疾病。申请人提出的项目侧重于多核破骨细胞的分化和活化，这些细胞吸收骨（Drs Horne，Kyriakides和Vignery）和成骨细胞，这些细胞制造骨，特别强调FGF和WNT下游信号传导（Drs Schlessinger，Gundberg，Horvath和Wu）。此外，班尼特博士还阐述了PTH激活成骨细胞的机制。这些研究人员共同涵盖了骨生物学的大型互补研究领域，将具有不同但互补的概念方法的部门聚集在一起，从而丰富了骨生物学领域。

DESCRIPTION (provided by applicant): The goal of this application is to provide Yale Medical School investigators that share an interest in bone biology with an instrument that will propel research in a necessary way. The instrument is a peripheral Quantitative Computerized Tomography (pQCT) that provides precise and rapid quantitative information about trabecular and cortical bone density in small live animals, such as mice and rats. Yale is the home of a large number of investigators who share an interest in bone biology, and who are more and more frequently translating their approach in vivo using rats and genetically engineered mice. These investigators are either well established, or new in the field of bone biology, and are associated with several departments at Yale School of Medicine, including the departments of Cell Biology, Immunobiology, Medicine, Orthopaedic Surgery, Pathology, Pharmacology, Comparative Medicine and Plastic Surgery. Yet, despite its large number of bone biologists, Yale is not equipped with state-of-the-art equipment to determine the quantity and the quality of bone in small animals that are used as experimental models. The specific aim of this application is therefore to acquire a pQCT to determine the trabecular and cortical bone density in live animals treated with a bone anabolic or anti catabolic drug/hormone, to assess the efficacy of the treatment. A pQCT can determine the trabecular and cortical bone density in live mice genetically engineered to either over express, or lack a specific gene that plays a central role in bone development, repair or homeostasis. A pQCT can also determine bone density in excised bones. The principal investigator and the key personnel of this application, together, are committed to further our understanding of the biology of bone with a common goal that is to prevent and repair bone loss, and treat diseases in which bone development and/or mass is altered. The projects proposed by the applicants focus on the differentiation and the activation of multinucleate osteoclasts, the cells that resorb bone (Drs Horne, Kyriakides and Vignery) and of osteoblasts, the cells that make bone, with specific emphasis on FGF and WNT downstream signaling (Drs Schlessinger, Gundberg, Horvath and Wu). In addition, the mechanism by which PTH activates osteoblasts is addressed by Dr Bennett. Together, these investigators cover large and complementary research fields in bone biology that bring together departments that have different, yet complementary conceptual approaches, and thereby enrich the field of bone biology.