Purchase of a 400 MHz Multi-Nuclear NMR Spectrometer

描述（由申请人提供）：本提案的目的是获得购买Varian 400 MHz多核NMR光谱仪的资金，以替代老化的Varian Gemini 200 MHz仪器。目前的光谱仪自1988年9月以来一直在连续使用。瓦里安将不再支持Gemini系列仪器。新仪器将是Ernest Mario药学院唯一的NMR光谱仪，将满足药物化学，药物科学，药理学和毒理学研究生课程约35-40名研究人员的需求。这些研究人员没有常规访问校园内的其他NMR设施。该工具将用于支持NIH资助的13个项目的研究工作。这些项目主要集中在新的杂环和大环抗癌剂的设计和合成，开发新的药物合成方法，具有抗癌或化学预防剂潜力的天然产物的分离和结构解析，以及化学预防剂作用机制的确定。所有这些项目都需要随时使用高分辨率NMR光谱仪进行结构测定。正在申请资金的瓦里安400 MHz多核NMR光谱仪将大大加强该仪器主要用户在新型抗癌药物设计、合成和发现领域的尖端研究工作。本建议书中所述的研究项目主要针对有可能发展成临床有用药物的新型抗癌药物的合成和鉴定、植物中生物活性化合物的分离和结构解析，以及天然存在的癌症预防剂的代谢产物和活化途径的测定。高分辨率多核NMR光谱仪的可用性是确定这些化合物的化学结构和推进这些研究工作的重要工具。

DESCRIPTION (provided by applicant): The objective of this proposal is to obtain funding for the purchase a Varian 400 MHz multi-nuclear NMR spectrometer as a replacement for an aging Varian Gemini 200 MHz instrument. The current spectrometer has been in continuous service since September 1988. Varian will no longer support the Gemini series of instruments. The new instrument will be the only NMR spectrometer in the Ernest Mario School of Pharmacy and will serve the needs of approximately 35-40 researchers from the Graduate Programs in Medicinal Chemistry, Pharmaceutical Science, and Pharmacology and Toxicology. These researchers have no routine access to other NMR facilities on campus. This instrument will be used to support research efforts on 13 NIH funded projects. These projects are focused largely on the design and synthesis of novel heterocyclic and macrocyclic anticancer agents, the development of new methodology for the synthesis of pharmaceutical agents, the isolation and structure elucidation of natural products having potential as anticancer or chemopreventive agents, and on the determination of the mechanisms of action of chemopreventive agents. All of these projects require ready access to a high resolution NMR spectrometer for structure determination. The Varian 400 MHz multi-nuclear NMR spectrometer for which funding is being requested will greatly enhance the cutting edge research efforts of the major users of this instrument in the areas of novel anticancer drug design, synthesis, and discovery. The research projects described in this proposal are largely targeted towards the synthesis and identification of novel anticancer agents with the potential for development into clinically-useful drugs, the isolation and structure elucidation of bioactive compounds from plants, and the determination of metabolites and activation pathways of naturally-occurring cancer-preventive agents. The availability of a high resolution multi-nuclear NMR spectrometer is an essential tool for determining the chemical structures of these compounds and advancing these research efforts.