Identification of odorant receptor-associated proteins.

气味受体相关蛋白的鉴定。

• 批准号: 7479711
• 负责人: HITOMI SAKANO
• 金额: $ 3.87万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7479711
• 关键词: Afferent Neurons; Alleles; Axon; Cilia; Co-Immunoprecipitations; Detection; Epithelium; Fellowship; In Situ Hybridization; Individual; Maps; Mass Spectrum Analysis; Mediating; Methods; Mus; Names; Nose; Odorant Receptors; Odors; Olfactory Epithelium; Pattern; Play; Proteins; Proteomics; Receptor Gene; Role; Stereotyping; axon guidance; insight; knockout gene; olfactory bulb; olfactory receptor; polyclonal antibody; receptor; research study; stem

DESCRIPTION (provided by applicant): Odor detection in mice is mediated by approximately 1000 different odorant receptors (ORs), which are expressed by olfactory sensory neurons (OSNs) in the olfactory epithelium (OE) of the nose. Each OSN expresses only one OR allele and OSNs with the same OR are scattered in the OE. However, their axons converge in a representative pair of glomeruli in the olfactory bulb (OB), producing a stereotyped map in which each glomerulus is dedicated to one OR. In addition to its role in recognizing odorants, there is growing evidence that the OR may also play a role in guiding the developing OSN axon to the correct target glomerulus in the OB. The evidence stems from OR gene swapping and deletion experiments and observations of OR protein in OSN axons. Is the axonal OR protein required for glomerular targeting? If so, what is the mechanism? We propose to investigate these questions. Using proteomic methods, we will identify proteins that are associated with ORs on axons versus cilia to explore the possible roles of axonal ORs. By doing so, we hope to better understand how axonal ORs may influence glomerular targeting.

描述（由申请人提供）： 小鼠的气味检测由大约1000种不同的气味受体（OR）介导，这些气味受体由鼻子的嗅觉上皮（OE）中的嗅觉感觉神经元（OSN）表达。每个OSN仅表达一个OR等位基因，具有相同OR的OSN分散在OE中。然而，它们的轴突会聚在嗅球（OB）中的代表性肾小球对中，产生刻板的地图，其中每个肾小球专用于一个OR。除了在识别气味中的作用外，越来越多的证据表明，OR还可能在引导发育中的OSN轴突到达OB中的正确靶肾小球中发挥作用。证据来源于OR基因交换和缺失实验以及OSN轴突中OR蛋白的观察。轴突OR蛋白是肾小球靶向所必需的吗？如果是，机制是什么？我们建议调查这些问题。使用蛋白质组学方法，我们将确定与轴突和纤毛上的OR相关的蛋白质，以探索轴突OR的可能作用。通过这样做，我们希望更好地了解轴突OR如何影响肾小球靶向。