The Effect of HIV and HAART on Oral Microbial Colonization and Diversityl

HIV 和 HAART 对口腔微生物定植和多样性的影响

• 批准号: 7291226
• 负责人: YIHONG LI
• 金额: $ 23.82万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7291226
• 关键词: AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Actinobacillus actinomycetemcomitans; Acute; Affect; Age; Anti-Bacterial Agents; Anti-Retroviral Agents; Antibiotic Therapy; Bacteria; Biological Assay; CD4 Lymphocyte Count; CD4 Positive T Lymphocytes; Candida; Case-Control Studies; Cell Count; Cessation of life; Chronic; Clinical; Combined Modality Therapy; Data; Defensins; Dental caries; Development; Disease; Environment; Gender; Genomics; Gingivitis; Goals; HIV; HIV Infections; HIV Seropositivity; Highly Active Antiretroviral Therapy; Host Defense; Individual; Infection; Lactobacillus; Lactoferrin; Lead; Measures; Methods; Microbe; Molecular; Mouth Diseases; Muramidase; Numbers; Opportunistic Infections; Oral; Oral cavity; Oral health; Patients; Periodontal Diseases; Persons; Polymerase Chain Reaction; Porphyromonas; Predisposition; Proteins; Public Health; Rate; Recombinant DNA; Research; Ribosomal DNA; Saliva; Salivary; Salivary Proteins; Sampling; Secretory Immunoglobulin A; Sequence Analysis; Streptococcus; Streptococcus mutans; Streptococcus sobrinus; System; Testing; Time; Treponema denticola; Viral; Viral Load result; Virus Diseases; antileukoprotease; base; cytokine; denaturing gradient gel electrophoresis; experience; human SLPI protein; insight; killings; microbial; microbial colonization; microbial community; prevent; programs; prospective; salivary proline-rich proteins

HIV/AIDS, killed 2.8 million persons in 2005, continues to be a major public health problem throughout the world. In the U.S. alone, approximately 40,000 persons become infected with HIV each year. Highly active antiretroviral combination therapy (HAART) has significantly reduced the number of AIDS deaths by 70% since 1996, and has changed the clinical profile of HIV infection from a sub-acute lethal to a chronic ambulatory disease. However, HIV-infected patients experience more mucosal opportunistic infections, more dental caries, and severe periodontal diseases, even after HAART, suggesting that compromised oral micro-environment within the oral cavity is a consequence of the viral infection. The long-term goal of this research is to elucidate the association among the innate host defense system, HIV infection, HAART, and oral microbiota. The objectives of the proposed study are to define the difference in oral microbiota between HIV-infected and non-infected individuals and to determine the effect of subsequent HAART on the dynamic changes of the microbial community in the oral cavity. Our central hypothesis is that HIV infection decreases the level of overall microbial colonization and diversity in the oral cavity and that HAART alters the microbiota so compromised. A corollary hypothesis is that the changes in microbial diversity in HIV+ individuals lead to an increase in cariogenic and periodontal bacteria, resulting in an increase in caries and periodontal disease. To test these hypotheses we will measure the colonization of (a) total cultivable microbes; (b) total Candida spp.; (c) total streptococci; and (d) total lactobacilli in saliva of HIV infected and noninfected subjects; examine the microbial diversity using the 16S rDNA-based approach; , and determine the association among specific salivary proteins of known involvement in HIV infection, specific cariogenic and periodontopathogenic bacterial colonization, and oral health assessments. The findings of this study will provide new insight into whether HIV infection causes changes in oral microbiota and salivary protein profiles favoring the development of caries and periodontal diseases and may the development of specific antibiotic therapy to prevent the adverse change in the microbiota and to decrease susceptibility to other infections

艾滋病毒/艾滋病在2005年造成280万人死亡，它仍然是一个主要的公共卫生问题