Molecular Epidemiological Approach to Determine S. mutans
确定变形链球菌的分子流行病学方法
基本信息
- 批准号:6899746
- 负责人:
- 金额:$ 7.38万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-07-01 至 2006-04-30
- 项目状态:已结题
- 来源:
- 关键词:Streptococcus infectionStreptococcus mutansbacterial DNAcommunicable disease transmissiondental cariesdisease /disorder etiologyhuman tissuelongitudinal human studymicroorganism classificationmolecular cloningnucleic acid probesnucleic acid purificationnucleic acid sequencepolymerase chain reactionrestriction fragment length polymorphismtechnology /technique development
项目摘要
DESCRIPTION (provided by applicant): Dental caries is the single most common chronic childhood disease. Each year, more than 51 million school hours are lost due to dental-related illness and over $40 billion is spent on the treatment of this disease. Epidemiological and clinical studies have suggested that mutans streptococci, particularly Streptococcus mutans, are the major microbial pathogens associated with dental caries. The most commonly used technique to identify S. mutans is cultivation on selective media. The major limitations of the method include inadequate detection of S. mutans in saliva particularly when S. mutans is present at low levels; morphology varies depending upon the medium used; and it is costly and labor-intensive. To date, the most reliable technique to rapidly and specifically identify bacterial species is PCR. But for S. mutans, the lack of species-specific probes and primers continues to limit high-throughput research on prevalence and colonization of S. mutans. Therefore, the objective of this project is to develop highly sensitive and species-specific probes and primers that can be used in PCR-based assays to rapidly, accurately, and effectively identify S. mutans in clinical oral specimens. Our goal will be accomplished by pursuing the following specific aims. (1) To identify potentially unique sequences in S. mutans genome that will enable us to develop species-specific probes and primers for the detection of S. mutans in the clinical specimens. (2) To validate the probes and to demonstrate the high sensitivity and specificity of the species-specific probes and primers. (3) To compare the species-specific probes and primers with the conventional culture method. From these experiments, we should be able to obtain well-defined S. mutans-specific probes and primers and to prove the superiority of the newly developed probes to the findings obtained from the culture method. The new molecular markers will enable us to conduct molecular epidemiological studies of S. mutans infection and high-throughput research so that we can improve our understanding of the polymicrobial etiology of dental caries, ascertain a child's risk potential prior to disease development, and evaluate the effectiveness of caries interventions. Application of the new molecular tools will have a substantial impact on improving the oral health of children.
描述(由申请人提供):龋齿是一种最常见的慢性儿童疾病。每年,超过5100万学时因与牙齿有关的疾病而损失,超过400亿美元用于治疗这种疾病。流行病学和临床研究表明,变形链球菌,特别是变形链球菌,是与龋齿相关的主要微生物病原体。最常用的鉴定S.变异体是在选择性培养基上培养的。该方法的主要局限性包括S.特别是当S.变形杆菌以低水平存在;形态根据所使用的培养基而变化;并且它是昂贵的和劳动密集的。迄今为止,快速和特异性鉴定细菌种类的最可靠技术是PCR。但对于S.变异链球菌,缺乏物种特异性探针和引物继续限制高通量研究的流行和殖民的S。变异体因此,本研究的目的是开发高灵敏度和物种特异性的探针和引物,用于PCR检测,以快速,准确,有效地鉴定S。临床口腔标本中的变异株。我们的目标将通过追求以下具体目标来实现。(1)为了鉴定S.变异链球菌基因组,这将使我们能够开发物种特异性探针和引物用于检测S。临床标本中的变异株。(2)验证探针,并证明种特异性探针和引物的高灵敏度和特异性。(3)将种特异性探针和引物与常规培养方法进行比较。从这些实验中,我们应该能够得到定义良好的S。突变体特异性探针和引物,并证明新开发的探针优于从培养方法获得的结果。新的分子标记将使我们能够进行分子流行病学研究的S。因此,我们希望通过对变形杆菌感染的研究和高通量研究,提高我们对龋齿的多微生物病因学的理解,在疾病发展之前确定儿童的潜在风险,并评估龋齿干预措施的有效性。新分子工具的应用将对改善儿童口腔健康产生重大影响。
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Mode of delivery, mutans streptococci colonization, and early childhood caries in three- to five-year-old Thai children.
- DOI:10.1111/cdoe.12013
- 发表时间:2013-06
- 期刊:
- 影响因子:2.3
- 作者:Pattanaporn K;Saraithong P;Khongkhunthian S;Aleksejuniene J;Laohapensang P;Chhun N;Chen Z;Li Y
- 通讯作者:Li Y
Effect of Antimicrobial Interventions on the Oral Microbiota Associated with Early Childhood Caries.
- DOI:
- 发表时间:2015-05
- 期刊:
- 影响因子:1.6
- 作者:Yihong Li;A. Tanner
- 通讯作者:Yihong Li;A. Tanner
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{{ truncateString('YIHONG LI', 18)}}的其他基金
The Effect of HIV and HAART on Oral Microbial Colonization and Diversityl
HIV 和 HAART 对口腔微生物定植和多样性的影响
- 批准号:
7668036 - 财政年份:2008
- 资助金额:
$ 7.38万 - 项目类别:
The Effect of HIV and HAART on Oral Microbial Colonization and Diversityl
HIV 和 HAART 对口腔微生物定植和多样性的影响
- 批准号:
7291226 - 财政年份:2007
- 资助金额:
$ 7.38万 - 项目类别:
Molecular epidemiological approach to determine S.mutans
确定变形链球菌的分子流行病学方法
- 批准号:
6824395 - 财政年份:2004
- 资助金额:
$ 7.38万 - 项目类别:
The Effect of HIV and HAART on Oral Microbial Colonization and Diversityl
HIV 和 HAART 对口腔微生物定植和多样性的影响
- 批准号:
8116973 - 财政年份:
- 资助金额:
$ 7.38万 - 项目类别:
The Effect of HIV and HAART on Oral Microbial Colonization and Diversityl
HIV 和 HAART 对口腔微生物定植和多样性的影响
- 批准号:
8291144 - 财政年份:
- 资助金额:
$ 7.38万 - 项目类别:
The Effect of HIV and HAART on Oral Microbial Colonization and Diversityl
HIV 和 HAART 对口腔微生物定植和多样性的影响
- 批准号:
7904311 - 财政年份:
- 资助金额:
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