Biochemical-Analysis Core

生化分析核心

• 批准号: 7464356
• 负责人: MYRON GOODMAN
• 金额: $ 15.33万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2013-07-13
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7464356
• 关键词: 2-Aminopurine; Anisotropy; Biochemical; Biological Assay; Chemicals; Complex; Condition; Data; Dinucleotide Repeats; Drug Formulations; Enzyme Kinetics; Fluorescence; Hand; High Pressure Liquid Chromatography; Kinetics; Label; Left; Measurement; Modification; Nucleosides; Nucleotides; Oxygen; Preparation; Radio; Reaction; Reporter; Services; Students; Techniques; Time; Training; analog; bisphosphonate; chemical synthesis; data acquisition; design; enzyme mechanism; fluorophore; innovation; inorganic phosphate; mutant; nucleotide analog; pol genes; protein purification; research study; stopped-flow fluorescence

Core B is a biochemical synthesis and analysis core that will provide indispensable services for all three projects. The synthetic component of Core B is designed to perform large scale synthesis, purification and analysis of nucleotide analogs for use in Projects 1 and 3. The biochemical component of Core B is designed to carry out protein purifications and to synthesize specialized DMA constructs required for Projects 1 and 3. The analysis component will be responsible for acquiring and performing global analysis on kinetic data, including presteady state stopped-flow fluorescence intensity and rotational anisotropy and rapid quench experiments, to be used in support of Projects 2 and 3. Core B will serve as a primary vehicle for training graduate and postdoctoral students in chemical synthesis and protein purification techniques and in the principles and practice of enzyme kinetic analysis.

核心B是一个生化合成和分析核心，将为这三个核心提供不可或缺的服务 项目。核心B的合成组件旨在执行大规模合成、提纯和 项目1和3中使用的核苷酸类似物的分析。核心B的生化成分是设计的 进行蛋白质提纯并合成项目1和3所需的专门的DMA结构。 分析组件将负责获取并执行对动力学数据的全局分析， 包括稳态前停流荧光强度、旋转各向异性和快速猝灭 用于支持项目2和3的实验。核心B将作为培训的主要工具 化学合成和蛋白质纯化技术研究生和博士后 酶动力学分析的原理与实践。