Biochemical-Analysis Core

核心B是一个生化合成和分析核心，将为所有三个提供不可或缺的服务 项目核心B的合成组分被设计为进行大规模合成、纯化和纯化。 分析用于项目1和3的核苷酸类似物。设计了核心B的生化组分 进行蛋白质纯化和合成项目1和3所需的专门的DMA构建体。 分析部分将负责获取动力学数据并进行全局分析， 包括稳态前的停流荧光强度、旋转各向异性和快速猝灭 实验，用于支持项目2和3。核心B将作为培训的主要工具 研究生和博士后学生在化学合成和蛋白质纯化技术和在 酶动力学分析原理与实践。

Core B is a biochemical synthesis and analysis core that will provide indispensable services for all three projects. The synthetic component of Core B is designed to perform large scale synthesis, purification and analysis of nucleotide analogs for use in Projects 1 and 3. The biochemical component of Core B is designed to carry out protein purifications and to synthesize specialized DMA constructs required for Projects 1 and 3. The analysis component will be responsible for acquiring and performing global analysis on kinetic data, including presteady state stopped-flow fluorescence intensity and rotational anisotropy and rapid quench experiments, to be used in support of Projects 2 and 3. Core B will serve as a primary vehicle for training graduate and postdoctoral students in chemical synthesis and protein purification techniques and in the principles and practice of enzyme kinetic analysis.