Biochemical-Analysis Core

核心B是一个生化合成和分析核心，将为这三个核心提供不可或缺的服务 项目。核心B的合成组件旨在执行大规模合成、提纯和 项目1和3中使用的核苷酸类似物的分析。核心B的生化成分是设计的 进行蛋白质提纯并合成项目1和3所需的专门的DMA结构。 分析组件将负责获取并执行对动力学数据的全局分析， 包括稳态前停流荧光强度、旋转各向异性和快速猝灭 用于支持项目2和3的实验。核心B将作为培训的主要工具 化学合成和蛋白质纯化技术研究生和博士后 酶动力学分析的原理与实践。

Core B is a biochemical synthesis and analysis core that will provide indispensable services for all three projects. The synthetic component of Core B is designed to perform large scale synthesis, purification and analysis of nucleotide analogs for use in Projects 1 and 3. The biochemical component of Core B is designed to carry out protein purifications and to synthesize specialized DMA constructs required for Projects 1 and 3. The analysis component will be responsible for acquiring and performing global analysis on kinetic data, including presteady state stopped-flow fluorescence intensity and rotational anisotropy and rapid quench experiments, to be used in support of Projects 2 and 3. Core B will serve as a primary vehicle for training graduate and postdoctoral students in chemical synthesis and protein purification techniques and in the principles and practice of enzyme kinetic analysis.