Innate Immune Gene Profiling Core

哮喘疾病研究的系统生物学方法的一个关键组成部分是 转录组和免疫介质。在过去的十年里，我们的小组发表了关于 利用各种cDNA分析技术，包括商业来源的微阵列， 如Affyoung和Agilent，以及为实验室定制开发的技术，如 代表性差异分析、差异显示分析和基因序列分析 表达（SAGE）。最近，由于我们需要包括几个新的基因，我们发现， 没有在任何商业阵列上表示，我们已经建立了自己的自定义阵列 专注于免疫和氧化反应中重要的基因。我们亦 研究了几个基因网络通路程序以支持数据分析。的主要目标 这一核心是将我们的专业知识扩展到U19项目的调查人员，并提供一个用户友好的， 一站式设施，用于广泛分析与免疫相关的cDNA和蛋白质阵列， 氧化应激，这是本提案中的两个突出问题。目标是：1.为了提供经过验证的， 为U19研究者提供灵敏和标准化的人类和小鼠cDNA谱分析技术。2. 为广泛的人和小鼠细胞因子/趋化因子和免疫蛋白的使用提供帮助 阵3.为了协助对cDNA和蛋白质阵列数据进行生物信息学分析， 核心将提供访问和协助使用计算机程序来分析数据， 途径程序，以协助解释数据，以了解其生物学意义。

A key component in the systems biology approach to the study of asthmatic disease is the profiling of transcriptome and immune mediators. Over the last decade, our group has published on the utilization of various cDNA profiling technology including microarrays from commercial sources, such as Affymetrix and Agilent, and technology developed custom to the lab, such as representational difference analysis, differential display analysis, and serial analysis of gene expression (SAGE). Recently due to our need to include several novel genes that we found which are not represented on any commercial arrays, we have established our own custom arrays focusing on genes important in immune and oxidative responses. In addition, we have also investigated several gene network- pathway programs to support data analysis. The main goal of this core is to extend our expertise to investigators in this U19 Project, and to provide a userfriendly, one-stop facility for the broad analysis of cDNA and protein arrays relevant to immunity and oxidative stress, two issues of prominence in this proposal. The goals are: 1. To provide validated, sensitive and standardized human and mouse cDNA profiling technology for U19 investigators. 2. To provide help in the usage of broad human and mouse cytokine/chemokine and immune protein arrays. 3. To assist in the bioinformatics analysis of data from the cDNA and protein arrays; the Core will provide access and assistance in using computer programs to analyze the data, and pathway programs to assist in the interpretation of data to understand its biological significance.