BAC Library Production and Maintenance

目的是制备10倍冗余的细菌人工染色体（BAG）文库， 由NIH咨询委员会选择，并根据NIH政策传播图书馆。的 图书馆将根据科学界对比较基因组学的兴趣进行调整，并用于 支持全基因组测序项目。该项目是早期合作项目的延续。 美国国立卫生研究院和儿童医院奥克兰研究所之间的协议。应用程序已 与制备（BAC）相关的四个组件：1）文库制备，2）克隆阵列和文库 重复，3）表征，4）研究与开发，以提高工艺效率和BAG 图书馆质量。在第一年，将为具有典型的 哺乳动物基因组大小。这相当于“800，000袋克隆”的制备能力。年间 第二和第三的计划，在袋施工方法的改进可能会导致略有增加， 吞吐量延续计划规模小，没有提供“规模经济”的机会 改进.新的图书馆将补充广泛的剧目袋克隆收藏已经 可用于比较基因组分析。新的克隆集合将由一个标准化的集合进行分析 测试，包括一组基因组标记筛选，有限的BAC末端测序和插入大小 保持战略定力一旦图书馆通过质量控制，新的资源将以 格式与主要应用程序一致。为了方便访问BAG图书馆，一项基于 已确定按使用费收回成本。预计新的BAG资源将继续 通过人类基因组的比较，有助于更好地了解人类基因的功能和进化。 基因与动物物种的光谱不断增长。BAG克隆也将越来越多地用作工具， 为人类疾病建立动物模型。克隆人的广泛传播 项目的组成部分。大多数图书馆的使用费不足以支付维护费用。因此，我们认为， 要求提供小额供资，以支持维持新图书馆和 上一个项目的倡议。通过BACPAC网站提供的图书馆信息 参考资料（bacpac.chori.org）。

The goal is to prepare tenfold redundant Bacterial Artificial Chromosomes (BAG) libraries for species selected by an NIH advisory committee and to disseminate the libraries in accordance with NIH policy. The libraries will be tailored to the scientific community's interest in comparative genomics and be used in support of whole-genome sequencing projects. The project is a continuation of an earlier cooperative agreement between the NIH and the Children's Hospital Oakland Research Institute. The application has four components relevant to preparing (BACs): 1) Library preparation, 2) Clone Arraying & Library Duplication, 3) Characterization, and 4) Research & Development to improve the process efficiency and BAG library quality. During the first year, four BAG libraries will be prepared for genomes having a typical mammalian genome size. This corresponds with an "800,000 BAG clone" preparation capacity. During years two and three of the program, improvements in BAG construction methods may result in slight increases in throughput. The small size of the continuation program does not offer opportunities for "economy of scale" improvements. The new libraries will complement the extensive repertoire of BAG clone collections already available for comparative genome analysis. The new clone collections will be analyzed by a standardized set of tests, including screening with a set of genomic markers, limited BAC-end sequencing and insert size determination. Once the libraries pass the quality controls, the new resources will be made available in a format consistent with major applications. To facilitate access to the BAG libraries, a screening service based on cost recovery by user fees has been established. It is expected that the new BAG resources will continue to contribute to better understanding of human gene function and evolution through comparison of human genes with a growing spectrum of animal species. BAG clones will also increasingly be used as tools to create animal models for human diseases. Widespread dissemination of the clones is an essential component of the project. User fees for most libraries cannot cover the costs of maintenance. Hence, mimimal funding is requested in support of the maintenance of the new libraries and those generated under the previous program initiative. Information about libraries available through the website for BACPAC Resources (http://bacpac.chori.org).