Mouse Breeding and Husbandry

小鼠育种核心（核心C）将产生，房屋，并保持所需的最终育种者，以获得计划项目资助（PPG）的所有项目所需的单一和组合基因靶向和转基因小鼠。一旦获得，这些动物将移交给PPG项目的个体研究者进行大规模饲养，以产生拟定研究所需的动物数量。动物综合体由兽医和委员会认证的兽医病理学家马克·A博士指导。他制定了动物护理和繁殖的政策。一名副主任负责管理10名技术人员的日常监督，这些技术人员负责照顾动物并建立所需的动物园。其中三个人被分配到动物的W.M. Keck转基因研究中心，将用于当前PPG的研究。该核心的职责是产生适当的小鼠，当研究者饲养时，将导致获得研究所需特定动物的统计概率较高。考虑到这些项目中最终动物的遗传菌株的复杂性， 首先需要生成最终动物。核心C将负责后一项任务。此核心的目标是至少生成：1）X[+/-]或X[-/-]（当具有这种基因型的小鼠能够存活和繁殖时）小鼠和X+/-/Y-/-小鼠（其中Y是第二基因）或X-/-/Y-/-用于大规模育种以产生Castellino项目所需的动物; 2）APC[Min+/-o]/X+/-（或X-/-，如果它们存活并繁殖）小鼠用于Ploplis项目的大规模繁殖;以及3）为罗森项目产生足够数量的WT和FX+/-繁殖动物。

The Mouse Breeding Core (Core C) will generate, house, and maintain the final breeders needed to obtain the single and combined gene targeted and transgenic mice needed for all projects of the Program Project Grant (PPG). Once obtained, these animals will be turned over to individual investigators of the projects of the PPG for large-scale breedings to generate the required numbers of animals for the studies proposed. The animal complex is directed by a veterinarian and board certified veterinary pathologist, Dr. Mark A. Suckow, who establishes the policies regarding animal care and breeding. An associate director manages day-to-day supervision of the ten technologists who care for the animals and establish the requested breedings. Three of these latter individuals are assigned to animals of the W.M. Keck Center for Transgene Research that will be used in the investigations of the current PPG. The responsibilities of this core will be to generate appropriate mice that when bred by investigators will lead to a high statistical probability of obtaining the specific animals needed for the studies. Given the complexities of the genetic strains of the final animals in these projects, strains with low probabilities of generating the final animals will be first required. Core C will assume responsibility for this latter task. The aims of this core are to generate at a minimum: 1) X[+/-] or X[-/-] (when mice with this genotype are able to survive and breed) mice and X+/-/Y-/- (where Y is a second gene) or X-/-/Y-/- for large-scale breedings to generate animals required for Project by Castellino; 2) APC[Min+/-o]/X+/- (or X-/-, if they survive and breed) mice for large-scale breedings for Project by Ploplis; and 3) to generate sufficient numbers of WT and FX+/- breeders for Project by Rosen.