Mouse Breeding and Husbandry

小鼠育种核心（Core C）将产生、饲养和维护最终育种者，以获得项目项目资助（PPG）所有项目所需的单一和组合基因靶向和转基因小鼠。一旦获得这些动物，这些动物将被移交给PPG项目的个别研究人员进行大规模繁殖，以产生所需数量的动物进行拟议的研究。动物综合体由兽医和委员会认证的兽医病理学家Mark a . Suckow博士指导，他制定了有关动物护理和繁殖的政策。一名副主管负责管理10名技术人员的日常监督，这些技术人员负责照顾动物并确定所要求的育种。后三个个体被分配到W.M.凯克转基因研究中心的动物身上，这些动物将用于目前PPG的研究。这个核心的职责将是产生合适的小鼠，当研究人员繁殖时，将导致获得研究所需的特定动物的高统计概率。考虑到这些项目中最终动物的遗传菌株的复杂性，低概率的菌株

The Mouse Breeding Core (Core C) will generate, house, and maintain the final breeders needed to obtain the single and combined gene targeted and transgenic mice needed for all projects of the Program Project Grant (PPG). Once obtained, these animals will be turned over to individual investigators of the projects of the PPG for large-scale breedings to generate the required numbers of animals for the studies proposed. The animal complex is directed by a veterinarian and board certified veterinary pathologist, Dr. Mark A. Suckow, who establishes the policies regarding animal care and breeding. An associate director manages day-to-day supervision of the ten technologists who care for the animals and establish the requested breedings. Three of these latter individuals are assigned to animals of the W.M. Keck Center for Transgene Research that will be used in the investigations of the current PPG. The responsibilities of this core will be to generate appropriate mice that when bred by investigators will lead to a high statistical probability of obtaining the specific animals needed for the studies. Given the complexities of the genetic strains of the final animals in these projects, strains with low probabilities of generating the final animals will be first required. Core C will assume responsibility for this latter task. The aims of this core are to generate at a minimum: 1) X[+/-] or X[-/-] (when mice with this genotype are able to survive and breed) mice and X+/-/Y-/- (where Y is a second gene) or X-/-/Y-/- for large-scale breedings to generate animals required for Project by Castellino; 2) APC[Min+/-o]/X+/- (or X-/-, if they survive and breed) mice for large-scale breedings for Project by Ploplis; and 3) to generate sufficient numbers of WT and FX+/- breeders for Project by Rosen.