Mouse Breeding and Husbandry

小鼠饲养及饲养

• 批准号: 7406637
• 负责人: FRANCIS J CASTELLINO
• 金额: $ 18.1万
• 依托单位: UNIVERSITY OF NOTRE DAME
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7406637
• 关键词: Animals; Breeding; Complex; Gene Targeting; Genes; Genotype; Housing; Individual; Investigation; Lead; Mus; Numbers; Pathologist; Policies; Probability; Program Research Project Grants; Research; Research Personnel; Supervision; Transgenes; Transgenic Mice; Veterinarians; animal care; day; genetic strain

The Mouse Breeding Core (Core C) will generate, house, and maintain the final breeders needed to obtain the single and combined gene targeted and transgenic mice needed for all projects of the Program Project Grant (PPG). Once obtained, these animals will be turned over to individual investigators of the projects of the PPG for large-scale breedings to generate the required numbers of animals for the studies proposed. The animal complex is directed by a veterinarian and board certified veterinary pathologist, Dr. Mark A. Suckow, who establishes the policies regarding animal care and breeding. An associate director manages day-to-day supervision of the ten technologists who care for the animals and establish the requested breedings. Three of these latter individuals are assigned to animals of the W.M. Keck Center for Transgene Research that will be used in the investigations of the current PPG. The responsibilities of this core will be to generate appropriate mice that when bred by investigators will lead to a high statistical probability of obtaining the specific animals needed for the studies. Given the complexities of the genetic strains of the final animals in these projects, strains with low probabilities of generating the final animals will be first required. Core C will assume responsibility for this latter task. The aims of this core are to generate at a minimum: 1) X[+/-] or X[-/-] (when mice with this genotype are able to survive and breed) mice and X+/-/Y-/- (where Y is a second gene) or X-/-/Y-/- for large-scale breedings to generate animals required for Project by Castellino; 2) APC[Min+/-o]/X+/- (or X-/-, if they survive and breed) mice for large-scale breedings for Project by Ploplis; and 3) to generate sufficient numbers of WT and FX+/- breeders for Project by Rosen.

小鼠育种核心（核心C）将产生、安置和维护获得计划项目拨款（PPG）所有项目所需的单一和组合基因靶向小鼠和转基因小鼠所需的最终育种者。一旦获得，这些动物将移交给 PPG 项目的个别研究人员进行大规模饲养，以产生拟议研究所需数量的动物。该动物综合体由兽医和委员会认证的兽医病理学家 Mark A. Suckow 博士领导，他制定了有关动物护理和繁殖的政策。一名副主任负责管理十名技术人员的日常监督，这些技术人员负责照顾动物并建立所要求的繁殖。后面的三个个体被分配给 W.M. 的动物。凯克转基因研究中心将用于当前 PPG 的研究。该核心的职责是产生合适的小鼠，当研究人员培育这些小鼠时，将有很高的统计概率获得研究所需的特定动物。考虑到这些项目中最终动物的遗传品系的复杂性，具有低概率的品系 首先需要生成最终的动物。核心 C 将承担后一项任务的责任。该核心的目标是至少生成：1) X[+/-] 或 X[-/-]（当具有该基因型的小鼠能够生存和繁殖时）小鼠和 X+/-/Y-/-（其中 Y 是第二个基因）或 X-/-/Y-/- 用于大规模繁殖，以生成 Castellino 项目所需的动物； 2) APC[Min+/-o]/X+/-（或 X-/-，如果它们存活并繁殖）小鼠，用于 Ploplis 项目的大规模繁殖； 3) 为罗森项目培育足够数量的 WT 和 FX+/- 育种者。