课题基金 / 基金详情

CORE--HISTOLOGY

CORE--HISTOLOGY
核心--组织学
批准号：
7264762
负责人：
Simon James Conway
金额：
$37.97万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
项目状态：
已结题
起止时间：
2007-04-01 至 2012-03-31
来源：
https://reporter.nih.gov/project-details/7264762
关键词：
ActinsAlcian BlueBromodeoxyuridineCryopreservationEquipmentFast GreenHeartHematoxylin and Eosin Staining MethodHistologicHistologyImmuneIn Situ HybridizationIn Situ Nick-End LabelingMusMyosin Heavy ChainsParaffin EmbeddingReagentResourcesStaining methodStainsStandards of Weights and MeasuresTechnical ExpertiseTrichrome stain methodcostresearch study

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Core C is the Resources Core. All three Projectswill rely extensively on histological analysis of mouse hearts. Centralization of equipment, technical expertise and reagents will facilitate these activities and minimize the associated costs. Specific activities of Core C include (1) assistance with routine paraffin embedding and sectioning, (2) assistance with routine cryopreservation and sectioning, (3) assistance with standard histochemical staining (i.e., Hematoxylin and Eosin, Alcian blue, Masson's trichrome, Sirius red and fast green, von Kossa, etc), (4) assistance with in situ hybridizations, and (5) assistance with routine immune histology analyses (i.e., TUNEL, BrdU, sarcomeric actin, myosin heavy chain, etc). Given the heavy utilization of histologic analyses by all three projects, Core C is essential for the successful completion of the proposed experiments.
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