CORE--HISTOLOGY

核心--组织学

• 批准号: 7901825
• 负责人: Simon James Conway
• 金额: $ 35.85万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7901825
• 关键词: Actins; Alcian Blue; Animal Model; Apoptosis; Arts; Bromodeoxyuridine; Cardiac; Cardiac Myocytes; Cardiovascular system; Cell Proliferation; Cessation of life; Collection; Cryopreservation; Data; Data Analyses; Data Set; Databases; Embryo; Equipment; Experimental Designs; Fast Green; Genetic; Heart; Heart failure; Hematoxylin and Eosin Staining Method; Histologic; Histology; Human; Human Resources; Immune; In Situ; In Situ Hybridization; In Situ Nick-End Labeling; Injury; Laboratories; Libraries; Modeling; Morphogenesis; Mus; Myosin Heavy Chains; Neonatal; Paraffin Embedding; Phenotype; Quality Control; Reagent; Records; Research; Research Personnel; Research Project Grants; Resources; Running; Sampling; Services; Specimen; Staging; Staining method; Stains; Supervision; Technical Expertise; Time; Tissue Sample; Training; Trichrome stain method; animal tissue; cost; design; instrument; instrumentation; meetings; programs; research study; tissue processing

Core C is the Resources Core. All three Projectswill rely extensively on histological analysis of mouse hearts. Centralization of equipment, technical expertise and reagents will facilitate these activities and minimize the associated costs. Specific activities of Core C include (1) assistance with routine paraffin embedding and sectioning, (2) assistance with routine cryopreservation and sectioning, (3) assistance with standard histochemical staining (i.e., Hematoxylin and Eosin, Alcian blue, Masson's trichrome, Sirius red and fast green, von Kossa, etc), (4) assistance with in situ hybridizations, and (5) assistance with routine immune histology analyses (i.e., TUNEL, BrdU, sarcomeric actin, myosin heavy chain, etc). Given the heavy utilization of histologic analyses by all three projects, Core C is essential for the successful completion of the proposed experiments.

Core C是资源核心。这三个项目都将广泛依赖于小鼠的组织学分析， 心中设备、技术专门知识和试剂的集中将促进这些活动， 最大限度地减少相关成本。核心C的具体活动包括（1）协助常规石蜡 包埋和切片，（2）辅助常规冷冻保存和切片，（3）辅助 标准组织化学染色（即，苏木精和伊红，阿尔辛蓝，马森三色，天狼星红， fast绿色，von Kossa等），（4）协助原位杂交，和（5）协助常规免疫 组织学分析（即，TUNEL、BrdU、肌节肌动蛋白、肌球蛋白重链等）。鉴于沉重的 由于所有三个项目都利用了组织学分析，核心C对成功完成 提出的实验。