Tissue Acquisition and Analysis

YSDRCC组织采集和分析核心（TAAC）的目的是刺激和促进 通过提供高效和具有成本效益的服务，设施和专家援助， 在正常和患病皮肤样本的采集和分析中。TAAC将促进 从研究者提供的实验组织中制备和分析组织切片，或 从TAAC机构可获得的档案组织中提取。现有样本来源包括约500，000份活检样本 提交给耶鲁皮肤病理学的患病皮肤和血清样本的标本档案 服务，并且在关系数据库中通过诊断交叉引用。组织切片和核酸 从多种常见和罕见疾病中提取的酸可以提供给研究人员， 组织库，按照耶鲁大学人类调查委员会的指导方针和HIPAA规定。 与这种独特组合相关的专业知识和样本量将使精通 提供以下特定服务，包括但不限于：（1）准备未染色组织 切片和组织化学染色切片;（2）免疫组织化学和免疫荧光染色， 组织切片;（3）双色流式细胞术分析和样品制备，包括分离和 新鲜白细胞和培养细胞以及从新鲜表皮分离的细胞的染色;（4）专家 协助组织切片的形态分析，包括组织化学和免疫组织化学 研究;（5）对研究人员进行皮肤常规处理中使用的各种技术的培训， 特殊染色技术的性能，如免疫过氧化物酶和免疫荧光;（6） 通过本核心和国际组织之间的积极合作， 病理学系关键技术单元的几个模块;（7）组织制备 组织微阵列的使用和性能;以及8）使用和获得新的最先进技术的培训 成像技术，包括激光捕获解剖显微镜和活体（双光子）显微镜。 对该核心项目的资助将通过提供适当的样本， 研究人员，包括那些主要感兴趣的领域不是皮肤的人，并通过提供 规模和最高水平的专业知识的调查。

The purposes of the YSDRCC Tissue Acquisition and Analysis Core (TAAC) are to stimulate and facilitate cutaneous research by providing highly efficient and cost-effective services, facilities, and expert assistance in the acquisition and analysis of samples of normal and diseased skin. The TAAC will facilitate the preparation and analysis of tissue sections derived from experimental tissues provided by investigators, or from archival tissue accessible to the TAAC facility. Sample sources in hand consist of ~500,000 biopsy specimen archive of diseased skin and serum samples that were submitted to the Yale Dermatopathology Service, and that are cross-referenced by diagnosis in a relational database. Tissue sections and nucleic acids extracted from multiple cases of common and rare diseases can be provided to researchers from this tissue bank, in accordance with Yale Human Investigation Committee guidelines and HIPAA regulations. The expertise and volume of samples associated with this unique combination will allow the proficient delivery of the following specific services, including, but not limited to: (1) preparation of unstained tissue sections and histochemical stained sections; (2) immunohistochemical and immunofluorescence staining of tissue sections; (3) dual-color flow cytometric analysis and sample preparation, including the isolation and staining of fresh leukocytes and cultured cells, as well as cells isolated from fresh epidermis; (4) expert assistance in the morphologic analysis of tissue sections, including histochemical and immunohistochemical studies; (5) training of investigators in a variety of techniques used in the routine processing of skin, the performance of special staining techniques such as immunoperoxidase and immunofluorescence; (6) access to fresh-frozen banked skin and related tissues through active collaboration between this Core and several modules of the Critical Technologies unit of the Department of Pathology; (7) preparation of tissue for and performance of tissue microarrays; and 8) training in the use of, and access to new, state-of-the art imaging technologies, including laser capture dissection microscopy and intravital (two-photon) microscopy. Funding of this Core will encourage investigation of skin diseases by providing appropriate samples to researchers, including those whose primary area of interest is not the skin, and by providing economies of scale and the highest levels of expertise to investigators.