Proteomic Biomarkers for Primary Open Angle Glaucoma

描述（由申请人提供）：原发性开角型青光眼（POAG）是全球老年人群致盲的主要原因之一。减缓或预防这种疾病的进展是一个紧迫的公共卫生目标。我们假设，生物标志物存在于易患POAG的个体的血液中，这将允许在疾病的临床证据之前识别出那些有风险的人。提出了一项病例对照初步研究，以检验血浆中含有POAG诊断生物标志物的假设。将从来自美国的临床记录的患者中采集血浆，分为4个研究组，包括对照组和3个POAG组（高眼压、可能的POAG和明确的POAG）。ELISA将用于筛选血浆中可能作为POAG血浆生物标志物的蛋白质氧化修饰。高分辨率MALDI TOF TOF和QTOF质谱将用于筛选血浆蛋白质组模式和/或确定POAG诊断血浆肽的序列。长期目标是开发用于临床医学的常规诊断技术，以预测POAG易感性并监测治疗效果，基本上就像今天用于心血管疾病风险管理的血清胆固醇测量一样。这项研究将导致早期检测的方法“POAG的风险”个人，新的方法来衡量POAG的治疗效果和新的见解青光眼病理机制。公共卫生相关性：原发性开角型青光眼（POAG）是青光眼的最常见形式，并且影响约300万美国人和全世界超过7000万人。减缓或预防这种疾病的进展是一个紧迫的公共卫生目标。这项研究将有助于早期发现“POAG高危”个体的方法，测量POAG治疗效果的新方法以及对青光眼病理机制的新见解。

DESCRIPTION (provided by applicant): Primary open angle glaucoma (POAG) is one of the leading causes of blindness in the elderly population worldwide. Slowing or preventing the progression of this disease is an urgent public health goal. We hypothesize that biomarkers exist in the blood of individuals susceptible to developing POAG that will allow identification of those at risk prior to clinical evidence of the disease. A case-control pilot study is proposed to test the hypothesis that plasma contains diagnostic biomarkers for POAG. Plasma will be collected from clinically documented patients from the United States in four study groups, including a control group and three POAG groups (ocular hypertension, possible POAG and definite POAG). ELISA will be used to screen plasma for protein oxidative modifications that may serve as POAG plasma biomarkers. High resolution MALDI TOF TOF and QTOF mass spectrometry will be used to screen plasma for proteomic patterns and/or to determine the sequence of POAG diagnostic plasma peptides. The long term goal is the development of routine diagnostic technology for use in clinical medicine to predict POAG susceptibility and to monitor therapeutic efficacy, essentially as serum cholesterol measurements are used today for risk management for cardiovascular disease. This research will lead to methods for early detection of "POAG at risk" individuals, new methods for measuring POAG therapeutic efficacies and new insights into mechanisms of glaucoma pathology. PUBLIC HEALTH RELEVANCE: Primary open angle glaucoma (POAG) is the most common form of glaucoma and affects about 3 million Americans and over 70 million people worldwide. Slowing or preventing the progression of this disease is an urgent public health goal. The proposed research will lead to methods for early detection of "POAG at risk" individuals, new methods for measuring POAG therapeutic efficacies and new insights into the mechanisms of glaucoma pathology.