The Zebrafish Model of Microphthalmia

斑马鱼小眼症模型

• 批准号: 7486812
• 负责人: JAREMA MALICKI
• 金额: $ 9.82万
• 依托单位: MASSACHUSETTS EYE AND EAR INFIRMARY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7486812
• 关键词: Affect; Animal Model; Anophthalmos; Candidate Disease Gene; Cell Proliferation; Child; Class; DNA; Defect; Development; Disease; Embryonic Development; Eye; Eye diseases; Genes; Genetic; Genetic Screening; Goals; Growth; Growth Disorders; Human; Individual; Lead; Malignant Neoplasms; Medical; Microphthalmos; Modeling; Molecular; Mutation; Nature; Normal tissue morphology; Numbers; Organism; Pattern; Phenotype; Regulation; Running; Sampling; Source; Vision; Zebrafish; base; insight; mutant; size

DESCRIPTION (provided by applicant): The regulation of tissue growth is of paramount importance for the understanding of vertebrate, including human, embryogenesis. It has also a tremendous medical significance. Deviations from normal tissue growth lead to severe patterning defects during embryogenesis, and to cancer in the mature organism. Abnormal patterns of cell proliferation produce a number of developmental eye disorders in humans. One of them is microphthalmia, which in the most extreme cases leads to the complete absence of eyes. To identify the genetic causes of human microphthalmia and anophthalmia, we chose to use an animal model, the zebrafish. Genetic screens in zebrafish identified many mutations that result in a decreased eye size. One of the most severe small eye phenotypes is caused by defects of the out of sight (out) locus. The goal of this project is to identify the molecular nature of the out gene, and to develop an understanding of its function on the cellular level. Once the molecular identity of this gene is known, we will investigate whether its defects are responsible for human microophthalmia by screeing DNA samples from diseased individuals. Similar animal model-based approaches have been very productive, leading to the identification of microphthalmia-causing mutations in several genes, including rx, six6, and sox2. This project is the first step on the way to use zebrafish mutants on a broader scale to identify the genetic causes of eye growth disorders. In the long run, the zebrafish is likely to become a major source of candidate genes to study this class of abnormalities. This strategy is likely to make a major contribution to the understanding of the genetic causes of microphthalmia and its more severe form, anophthalmia, in humans. We will use animal model, the zebrafish, to identify genes responsible for microphthalmia, a human eye disorder characterized by a reduction of eye size, and in the most extreme the absence of eyes. These studies will provide insight into the genetic basis of this debilitating disorder that affects 1 in 5,000 children.

描述（由申请人提供）：组织生长的调节对于理解脊椎动物（包括人类）胚胎发生至关重要。它也具有巨大的医学意义。与正常组织生长的偏差导致胚胎发生期间严重的图案缺陷，并导致成熟生物体中的癌症。细胞增殖的异常模式在人类中产生许多发育性眼部疾病。其中之一是小眼症，在最极端的情况下会导致完全没有眼睛。为了确定人类小眼症和无眼症的遗传原因，我们选择使用动物模型斑马鱼。斑马鱼的遗传筛查发现了许多导致眼睛尺寸减小的突变。最严重的小眼睛表型之一是由视线外（out）位点的缺陷引起的。该项目的目标是确定外基因的分子性质，并在细胞水平上了解其功能。一旦这个基因的分子身份是已知的，我们将调查其缺陷是否是负责人类小眼球通过筛选DNA样本从患病的个人。类似的基于动物模型的方法非常有成效，导致在几个基因中识别出引起小眼症的突变，包括feta 1，six 6和sox 2。该项目是在更大范围内使用斑马鱼突变体来确定眼睛生长障碍的遗传原因的第一步。从长远来看，斑马鱼很可能成为研究这类异常的候选基因的主要来源。这一策略可能会对理解人类小眼球及其更严重形式无眼球的遗传原因做出重大贡献。我们将使用动物模型，斑马鱼，以确定基因负责小眼，人类的眼睛疾病的特点是眼睛的大小减少，并在最极端的情况下，没有眼睛。这些研究将深入了解这种影响1/5000儿童的衰弱性疾病的遗传基础。