A cDNA Sequence Resource for Baboon

狒狒 cDNA 序列资源

• 批准号: 7482235
• 负责人: Roger E. Bumgarner
• 金额: $ 57.59万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7482235
• 关键词: Adult; African; Alcoholic Liver Diseases; Alleles; American Type Culture Collection; Animal Model; Animals; Atherosclerosis; Atlases; Bioinformatics; Biological Sciences; Biomedical Research; Biotin; Brain; Brain region; Cerebellum; Characteristics; Chromosome Mapping; Collaborations; Collection; Communities; Comparative Genomic Analysis; Complementary DNA; Computer software; Custom; DNA Microarray Chip; DNA Microarray format; DNA Sequence; Data; Data Analyses; Databases; Deposition; Development; Exhibits; Foundations; Genbank; Gene Expression; Generic Drugs; Genes; Genome; Genome Mappings; Genomics; Goals; Histocompatibility Testing; Homologous Gene; Human; Human Biology; Human Genome; Hybrids; Imagery; Individual; Information Distribution; Institutes; Label; Length; Letters; Libraries; Light; Macaca; Maps; Medicine; Mental disorders; Messenger RNA; Methods; Mitochondrial DNA; Modeling; Molecular; Monitor; Numbers; Oligonucleotides; Organism; Osteoporosis; Pan Genus; Pancreatic ribonuclease; Papio; Papio anubis; Papio hamadryas; Parents; Physiological; Placenta; Positioning Attribute; Primates; Principal Investigator; Process; Proteins; Proteomics; RNA; RNA Splicing; Range; Rate; Relative (related person); Research; Research Design; Research Personnel; Resources; Scientist; Sequence Homology; Signal Transduction; Site; Streptavidin; System; Systems Analysis; Techniques; Technology; Testing; Thalamic structure; Tissues; Transcript; Travel; Universities; Variant; Washington; Work; cDNA Library; comparative; cost; design; design and construction; experience; fetal; frontal lobe; functional genomics; insulin sensitivity; interest; magnetic beads; nonhuman primate; professor; programs; reconstruction; repository; success; tool

DESCRIPTION (Provided by applicant): The primary goal of this application is to generate genomic resources for use by the primate research community and specifically by those researchers performing studies with baboons. At present (June 2005), there are a total of 2496 DNA sequences in Genbank from all baboon species combined. Of these, approximately 1,532 sequences were obtained from Papio anubis (olive baboon) and 639 were obtained from Papio hamadryas (hamadryas baboon). Many of these sequences are redundant - e.g. they represent the same gene (or region of mitochondrial DNA). Hence, there are fewer than 1000 unique baboon gene sequences represented in GenBank. The lack of molecular sequence information for baboon is troubling as the baboon is widely used in biomedical research. Nearly all of these studies will benefit both prospectively and retrospectively from the elaboration of the Baboon genome and related functional genomic studies. Moreover, comparative genomics studies between Baboon and Humans will allow us to determine the relevance of these animal studies to human biology and medicine. Finally, genome data are critical to the application of many functional genomics technologies and to molecular studies in Baboon. In particular, accurate sequence is absolutely necessary for the application of mass spectrometric methods of protein identification and to the design of DNA microarrays. In this application we propose to 1) Create normalized cDNA libraries from placenta, fetal brain and three regions of adult brain, 2) Sequence approximately 400,000 total clones 3) Create a database of annotated sequences and primate homologues, map the sequences to the human and chimp assemblies and identify likely SNP's in our sequences and 4) Create publicly available microarray designs for use by the baboon research community.

描述（由申请人提供）： 这个应用程序的主要目标是产生灵长类动物研究使用的基因组资源 社区，特别是那些用狒狒进行研究的研究人员。目前（2005年6月），基因库中共有2496个来自所有狒狒物种的DNA序列。其中，约1，532个序列来自Papio anubis（橄榄狒狒），639个序列来自Papio hamadryas（hamadryas狒狒）。这些序列中的许多是冗余的-例如，它们代表相同的基因（或线粒体DNA的区域）。因此，在GenBank中只有不到1000个狒狒基因序列。狒狒的分子序列信息的缺乏令人不安，因为狒狒被广泛用于生物医学研究。几乎所有这些研究都将受益于狒狒基因组和相关功能基因组研究的阐述。 此外，狒狒和人类之间的比较基因组学研究将使我们能够确定这些动物研究与人类生物学和医学的相关性。 最后，基因组数据对于许多功能基因组学技术的应用和狒狒的分子研究至关重要。 特别是，准确的序列是绝对必要的应用质谱方法的蛋白质鉴定和DNA微阵列的设计。 在本申请中，我们提出：1）从胎盘、胎脑和三种哺乳动物中创建标准化cDNA文库。 成年人大脑的区域，2）对大约400，000个总克隆进行测序，3）创建注释序列和灵长类同源物的数据库，将序列映射到人类和黑猩猩组件，并鉴定我们的序列中可能的SNP，以及4）创建公开可用的微阵列设计，供狒狒研究团体使用。