A cDNA Sequence Resource for Baboon

描述(由申请人提供)： 这个应用程序的主要目标是生成基因组资源，供灵长类研究社区使用，特别是供那些对狒狒进行研究的研究人员使用。目前(2005年6月)，在Genbank中总共有2496个序列，来自所有的狒狒物种。其中，大约1,532个序列是从Papio Anubis(橄榄狒狒)中获得的，639个序列是从Papio hamadryas(Hamadryas Baboon)中获得的。这些序列中的许多都是冗余的--例如，它们代表相同的基因(或线粒体DNA的区域)。因此，在GenBank中只有不到1000个独特的狒狒基因序列。由于狒狒在生物医学研究中的广泛应用，其分子序列信息的缺乏是令人担忧的。几乎所有这些研究都将前瞻性和回溯性地受益于浣熊基因组的精化和相关的功能基因组研究。此外，浣熊和人类之间的比较基因组学研究将使我们能够确定这些动物研究与人类生物学和医学的相关性。最后，基因组数据对于许多功能基因组学技术的应用以及对猴宝宝的分子研究都是至关重要的。特别是，准确的序列对于蛋白质鉴定的质谱学方法的应用和DNA微阵列的设计是绝对必要的。 在这项应用中，我们建议1)从胎盘、胎脑和成人脑的三个区域创建标准化的cDNA文库，2)对大约400,000个总克隆进行测序，3)创建注释序列和灵长类同源物的数据库，将序列映射到人类和黑猩猩的装配，并在我们的序列中识别可能的SNP，以及4)创建可公开使用的微阵列设计，供狒狒研究社区使用。

DESCRIPTION (Provided by applicant): The primary goal of this application is to generate genomic resources for use by the primate research community and specifically by those researchers performing studies with baboons. At present (June 2005), there are a total of 2496 DNA sequences in Genbank from all baboon species combined. Of these, approximately 1,532 sequences were obtained from Papio anubis (olive baboon) and 639 were obtained from Papio hamadryas (hamadryas baboon). Many of these sequences are redundant - e.g. they represent the same gene (or region of mitochondrial DNA). Hence, there are fewer than 1000 unique baboon gene sequences represented in GenBank. The lack of molecular sequence information for baboon is troubling as the baboon is widely used in biomedical research. Nearly all of these studies will benefit both prospectively and retrospectively from the elaboration of the Baboon genome and related functional genomic studies. Moreover, comparative genomics studies between Baboon and Humans will allow us to determine the relevance of these animal studies to human biology and medicine. Finally, genome data are critical to the application of many functional genomics technologies and to molecular studies in Baboon. In particular, accurate sequence is absolutely necessary for the application of mass spectrometric methods of protein identification and to the design of DNA microarrays. In this application we propose to 1) Create normalized cDNA libraries from placenta, fetal brain and three regions of adult brain, 2) Sequence approximately 400,000 total clones 3) Create a database of annotated sequences and primate homologues, map the sequences to the human and chimp assemblies and identify likely SNP's in our sequences and 4) Create publicly available microarray designs for use by the baboon research community.