Immunogenetics of Primates Used for Bioterror Research

非人灵长类动物在涉及A-C类生物防御病原体的研究中发挥了重要作用。食蟹鱼 短尾猴特别受欢迎，因为它们相对便宜且分布广泛。 可用。然而，人们对食蟹猴的免疫遗传学知之甚少，这使得 病原体特异性细胞免疫反应的评估。 具体目标1：我们将确定至少60个主要组织相容性复合体(MHC)I类和50个 食蟹猴表达的MHC-II类分子及其高通量表达方法的研究 这些等位基因不分型。食蟹猴的MHC I类和II类等位基因可能存在差异 来自不同国家的，所以我们将检测来自中国，毛里求斯，越南， 印度尼西亚和菲律宾。超过12000只食蟹猴是从这些国家进口的 2003财年。 在具体目标2中，我们将为10种常见的MHC I类和10种常见的MHC开发初步的多肽结合基序 在特定目的中发现的常见MHC II类等位基因1.如果来自不同地区的食蟹猴 有不同的MHC等位基因谱系，我们将重点定义为小巷发现的多肽结合基序 在中国，毛里求斯和越南猕猴。超过75%的食蟹猴是进口的 在2003财年从这些国家中的一个国家进入美国。 经验丰富的分子免疫遗传学家和蛋白质生物化学家在这方面的优势结合在一起 建议，实现食蟹猴主要组织相容性的高通量表征 复合体(MHC)分子和与其结合的病原体衍生的多肽。这些研究将有助于 物种特异性试剂(如MHC：多肽四聚体)的发展，以扩大其用途 食蟹猴临床前疫苗试验和疾病发病机制研究。

Nonhuman primates are instrumental in research involving Category A-C biodefense pathogens. Cynomolgus macaques (M. Fascicularis) are particularly desireable because they are comparitively inexpensive and widely available. However, little is known about the immunogenetics ot Cynomolgus macaques, complicating the evaluation of pathogen-specific cellular immune responses. in Specific Aim 1: Wewill identify at least 60 Major Histocompatibility Complex (MHC) class I and 50 MHC class II molecules expressed by Cynomolgus macaques and develop methods for high-throughput ge- notyping for these alleles. MHC class I and class II alleles may vary among Cynomolgus macaques obtained from different countries, so we will examine the immunogenetics of animals from China, Mauritius, Vietnam, Indonesia, and the Phillipines. More than 12,000 Cynomolgus macaques were imported from these countries in FY2003. In Specific Aim 2, we will develop preliminary peptide binding motifs for 10common MHC class I and 10 common MHC class II alleles identified in Specific Aim 1. If Cynomolgus macaques from different regions have distinct MHC allele repertoires, we will focus on the definition of peptide binding motifs for alleies found in Chinese, Mauritian, and Vietnamese macaques. More than 75% of the Cynomolgus macaques imported into the United States in FY2003 were brought from one of these countries. The strengths of experienced molecular immunogeneticists and protein biochemists a-e combined in this proposal, enabling the high-throughput characterization of Cynomolgus macaque major histocompatibility complex (MHC) molecules and the pathogen-derived peptides that bind to them. These studies will facilitate the development of species-specific reagents (such as MHC: peptide tetramers) that expand the utility of Cynomolgus macaques in preclinical vaccine trials and studies of disease pathogenesis.