Gene Expression Arrays for the Laboratory Opossum

灰色的短尾负鼠，Monodelphis arctica（也被称为“实验室负鼠”），是 世界上主要的实验室饲养的研究有袋动物，用于广泛的调查 一系列与人类发育、生理学和疾病易感性相关的主题。过去三 多年来，在建立基因组资源基础方面取得了巨大进展， 在比较基因组学、基因和基因组结构领域对该物种进行调查的途径，以及 影响发育和生理表型的基因的检测和作图。等 资源包括：一个完全排列的，公开可用的细菌人工染色体（BAG）库; 扩展的连锁图谱;以及即将完成的全基因组序列。相比之下，现代工具 功能基因组分析（基因调控和表达）是非常缺乏这一点和所有其他 有袋类动物鉴于目前正在进行的基础生物学和健康相关研究的多样性， 与M。在南极洲，迫切需要可用于调查 在正常和异常的生理和发育状态中的基因表达，以及在表现出 对疾病状况和发育异常的不同易感性。的根本目标 本申请中提出的研究是构建一系列具有广泛代表性的表达 序列（cDNA）文库和相应的高密度基因表达阵列，其可用于 基因表达谱的多样性的基础和生物医学为导向的研究应用，使用M。 北极熊和其他有袋动物。拟议的研究还将建立一个表达序列 标签（EST）数据库，并使用该数据库构建M. 这将揭示有袋动物基因组之间在组织和基因内容上的差异 和真兽目哺乳动物，特别是人类基因组。拟议研究的一个明确目标是 使这些cDNA文库、基因阵列和数据库资源完全可用于研究 社区

The gray, short-tailed opossum, Monodelphis domestica (also known as the "laboratory opossum"), is the predominant laboratory-bred research marsupial in the world and is used in investigations that span a broad range of topics relevant to human development, physiology, and disease susceptibility. In the past three years, excellent progress has been made In establishing a genomic resource base that is enabling new avenues of inquiry with this species in the areas of comparative genomics, gene and genome structure, and the detection and mapping of genes that influence developmental and physiologic phenotypes. Such resources include: a fully arrayed, publicly available bacterial artificial chromosome (BAG) library; an expanding linkage map; and a soon-to-be-completed full-genome sequence. In contrast, tools for modern functional genomic analysis (gene regulation and expression) are sorely lacking for this and all other marsupial species. Given the diversity of basic biological and health-related research currently being conducted with M. domestica, there is a critical need for tools that can be used for investigating variation in gene expression in normal and abnormal physiologic and developmental states, and in animals that exhibit varying susceptibilities to disease conditions and developmental anomalies. The fundamental objective of the studies proposed in this application is the construction of a broadly representative series of expressed sequence (cDNA) libraries and corresponding high-density gene expression arrays that can be utilized for gene expression profiling in a diversity of basic and biomedically oriented research applications using M. domestica and other marsupial species. The proposed research will also establish an expressed sequence tag (EST) database and use this database for the construction of an expressed sequence map of M. domestica that will reveal differences in organization and gene content between the genomes of marsupial and eutherian mammals, particularly the human genome. An explicit objective of the proposed study is to make these cDNA libraries, gene arrays, and database resources fully accessible to the research community.