Gene Expression Arrays for the Laboratory Opossum

实验室负鼠基因表达阵列

• 批准号: 6923548
• 负责人: PAUL B SAMOLLOW
• 金额: $ 30.56万
• 依托单位: TEXAS A&M UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6923548
• 关键词: animal genetic material tag; blood lipoprotein metabolism; cholesterol; chromosomes; developmental genetics; dietary lipid; family genetics; fluorescent in situ hybridization; genetic mapping; genetic markers; genetic models; genetic polymorphism; genetic recombination; genetic regulation; genome; genotype; hypercholesterolemia; linkage mapping; model design /development; nucleic acid sequence; nutrition related tag; opossums; polymerase chain reaction; quantitative trait loci; tissue /cell culture

DESCRIPTION (provided by applicant): The gray, short-tailed opossum, Monodelphis domestica (also known as the "laboratory opossum"), is the predominant laboratory-bred research marsupial in the world and is used in investigations that span a broad range of topics relevant to human development, physiology, and disease susceptibility. In the past three years, excellent progress has been made In establishing a genomic resource base that is enabling new avenues of inquiry with this species in the areas of comparative genomics, gene and genome structure, and the detection and mapping of genes that influence developmental and physiologic phenotypes. Such resources include: a fully arrayed, publicly available bacterial artificial chromosome (BAC) library; an expanding linkage map; and a soon-to-be-completed full-genome sequence. In contrast, tools for modern functional genomic analysis (gene regulation and expression) are sorely lacking for this and all other marsupial species. Given the diversity of basic biological and health-related research currently being conducted with M. domestica, there is a critical need for tools that can be used for investigating variation in gene expression in normal and abnormal physiologic and developmental states, and in animals that exhibit varying susceptibilities to disease conditions and developmental anomalies. The fundamental objective of the studies proposed in this application is the construction of a broadly representative series of expressed sequence (cDNA) libraries and corresponding high-density gene expression arrays that can be utilized for gene expression profiling in a diversity of basic and biomedically oriented research applications using M. domestica and other marsupial species. The proposed research will also establish an expressed sequence tag (EST) database and use this database for the construction of an expressed sequence map of M. domestica that will reveal differences in organization and gene content between the genomes of marsupial and eutherian mammals, particularly the human genome. An explicit objective of the proposed study is to make these cDNA libraries, gene arrays, and database resources fully accessible to the research community.

描述(由申请人提供)： 灰色短尾负鼠，家养单尾负鼠(又称实验室负鼠)，是世界上主要的实验室培育的有袋类研究动物，用于与人类发育、生理和疾病易感性相关的广泛主题的调查。在过去的三年里，在建立基因组资源库方面取得了很好的进展，这使得在比较基因组学、基因和基因组结构以及影响发育和生理表型的基因的检测和定位方面与该物种进行研究的新途径成为可能。这些资源包括：一个排列齐全的公开可用的细菌人工染色体(BAC)文库；一个扩展的连锁图谱；以及即将完成的全基因组序列。相比之下，现代功能基因组分析(基因调控和表达)的工具对这种和所有其他有袋类物种来说都非常缺乏。鉴于目前与家蝇进行的基础生物学和健康相关研究的多样性，迫切需要能够用于研究正常和异常生理和发育状态下的基因表达变化的工具，以及对疾病条件和发育异常表现出不同易感性的动物。本申请中提出的研究的基本目标是构建具有广泛代表性的一系列表达序列(CDNA)文库和相应的高密度基因表达阵列，这些表达序列文库和相应的高密度基因表达阵列可用于利用家蝇和其他有袋类物种进行各种基础和生物医学研究中的基因表达谱分析。这项拟议的研究还将建立一个表达序列标签(EST)数据库，并利用该数据库构建家蝇的表达序列图，揭示有袋类和真兽类哺乳动物基因组，特别是人类基因组在组织和基因含量方面的差异。这项拟议研究的一个明确目标是使这些cdna文库、基因阵列和数据库资源完全可供研究界使用。