Translation Grant for The HIPER project - Bringing the NMR Paradigm to ESR

HIPER 项目翻译资助 - 将 NMR 范式引入 ESR

• 批准号: EP/F039034/1
• 负责人: Graham Smith
• 金额: $ 126.71万
• 依托单位: University of St Andrews
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FF039034%2F1
• 关键词: Translation; Grant; HIPER; project; Bringing

In the original Basic Technology program Bringing the NMR Paradigm to ESR technology was developed to deliver complex sequences of very high power mm-wave pulses with sub-nanosecond resolution to a small sample in a strong magnetic field and measure a response from the sample 13 orders of magnitude lower in power, within 1 nanosecond of the final pulse. This was widely regarded as impossible by many leading members of the ESR instrumentation community three years ago. However, after two years developing the necessary mm-wave technologies and one year integrating them into a fully working system, the instrument is now demonstrating these very high levels of performance.The success of that program now opens up major new opportunities in the understanding of fast chemical and biochemical processes and in dramatically improving the sensitivity and resolution of techniques to understand local structure around paramagnetic centres, which often play a central role in determining the catalytic, enzymatic, electronic and optical properties of important chemical, biological and technological systems. It also promises to significantly improve the sensitivity of Site-Directed Spin Labelling techniques that promise to become a generic tool in the understanding of the structure, dynamics, conformational changes, interactions and function of important biomolecules in complex biomolecular processes. The aim of this Translation Grant is to provide underpinning but flexible support that will allow us to fully exploit this technological breakthrough via a series of pilot projects and collaborations.

在最初的基础技术计划中，将NMR范式引入ESR技术被开发用于在强磁场中向小样品提供具有亚纳秒分辨率的非常高功率毫米波脉冲的复杂序列，并在最终脉冲的1纳秒内测量样品的响应，其功率低13个数量级。三年前，ESR仪器社区的许多领导成员普遍认为这是不可能的。然而，经过两年的开发必要的毫米波技术和一年的集成到一个完整的工作系统，该仪器现在展示了这些非常高的性能水平。该计划的成功现在开辟了重大的新机会，在理解快速化学和生物化学过程，并在显着提高技术的灵敏度和分辨率，以了解顺磁中心周围的局部结构，其通常在确定重要化学、生物和技术系统的催化、酶、电子和光学性质中发挥核心作用。它还有望显着提高定点自旋标记技术的灵敏度，有望成为理解复杂生物分子过程中重要生物分子的结构，动力学，构象变化，相互作用和功能的通用工具。这项翻译补助金的目的是提供基础但灵活的支持，使我们能够通过一系列试点项目和合作充分利用这一技术突破。

项目成果

DEER Sensitivity between Iron Centers and Nitroxides in Heme-Containing Proteins Improves Dramatically Using Broadband, High-Field EPR.

• DOI: 10.1021/acs.jpclett.6b00456
• 发表时间: 2016-04-21
• 期刊: The journal of physical chemistry letters
• 作者: Motion CL;Lovett JE;Bell S;Cassidy SL;Cruickshank PA;Bolton DR;Hunter RI;El Mkami H;Van Doorslaer S;Smith GM
• 通讯作者: Smith GM

The histone chaperones Nap1 and Vps75 bind histones H3 and H4 in a tetrameric conformation.

• DOI: 10.1016/j.molcel.2011.01.025
• 发表时间: 2011-02-18
• 期刊: Molecular cell
• 影响因子: 16
• 作者: Bowman A;Ward R;Wiechens N;Singh V;El-Mkami H;Norman DG;Owen-Hughes T
• 通讯作者: Owen-Hughes T

Geometric model-based fitting algorithm for orientation-selective PELDOR data

• DOI: 10.1080/00268976.2014.960494
• 发表时间: 2015-03-19
• 期刊: MOLECULAR PHYSICS
• 影响因子: 1.7
• 作者: Abdullin, Dinar;Hagelueken, Gregor;Schiemann, Olav
• 通讯作者: Schiemann, Olav

Compact Wideband Corrugated Feedhorns With Ultra-Low Sidelobes for Very High Performance Antennas and Quasi-Optical Systems

• DOI: 10.1109/tap.2013.2243097
• 发表时间: 2013-01
• 期刊: IEEE Transactions on Antennas and Propagation
• 影响因子: 5.7
• 作者: J. McKay;D. Robertson;P. Cruickshank;R. Hunter;D. Bolton;R. Wylde;Graham M. Smith

Analysis of the Intrinsically Disordered N-Terminus of the DNA Junction-Resolving Enzyme T7 Endonuclease I: Identification of Structure Formed upon DNA Binding.

• DOI: 10.1021/acs.biochem.6b00242
• 发表时间: 2016-08-02
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Freeman AD;Stevens M;Declais AC;Leahy A;Mackay K;El Mkami H;Lilley DM;Norman DG
• 通讯作者: Norman DG