CORE--HISTOCHEMISTRY

为了加快Van Eldik、LaDu和Vassar项目中不可或缺的组织化学和细胞化学研究，我们将建立一个核心设施来提供组织服务、培训和专业知识。核心设施将配备两名技术人员，一名主任和一名副主任，并将配备小鼠脑切片和染色设备，然后可以通过附近细胞成像设施的许多技术进行检查。该核心将提供三种主要类型的服务：1)我们将对单个项目提供的小鼠大脑进行切片和染色，使用标准化的抗体和组织学试剂，包括活化标志物和神经变性标志物。2)我们将对项目人员进行适合其项目形态学方面的程序培训。这将包括光，共聚焦，和电子显微镜和相关的图像捕获和形态测量程序。这也将包括固定和染色方案，用于他们的实验与培养细胞。3)我们将在设计、执行和解释单个项目的组织学部分方面提供我们的专业知识。

In order to expedite the histochemical and cytochemical investigations that are integral to Projects by Van Eldik, LaDu and Vassar, will establish a core facility to provide histological services, training, and expertise. The core facility will be staffed by two technicians, a director, and a co-director and will be equipped for the sectioning and staining of mouse brain sections, which may then be examined by a number of techniques in the nearby Cell Imaging Facility. Three major types of service will be provided by this Core: 1) We will section and stain mouse brains provided by the individual projects, using a standardized panel of antibodies and histological reagents that includes markers of activation and markers of neurodegeneration. 2) We will train Project personnel in procedures appropriate to the orphological aspects of their Projects. This will include light, confocal, and electromicroscopical microscopy and relevant image-capture and morphometric procedures. This will also include fixation and staining protocols for use in their experiments with cultured cells. 3) We will make available our expertise in the design, execution, and interpretation of the histological portions of the individual Projects.