MOLECULAR BIOLOGY MODULE

分子生物学模块

• 批准号: 7286546
• 负责人: SUSANN M BRADY-KALNAY
• 金额: $ 9.88万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7286546
• 关键词: Agar Gel Electrophoresis; Appendix; Back; Bacteria; Bacterial Transformation; Bioinformatics; Breeding; Cancer Center Support Grant; Cancer Grant Supplements (P30); Cloning; Communication; Complementary DNA; DNA; DNA Ligation; Digestion; Electroporation; Ensure; Equipment; Funding; Gel; Gene Expression; Generations; Genes; Genotype; Heterozygote; Human Resources; Image; Institutes; Molecular Biology; Mouse Strains; Mus; Mutant Strains Mice; Numbers; Pathology; Plasmids; Polymerase Chain Reaction; Procedures; Proteins; Protocols documentation; RNA; Reaction; Reporting; Research Personnel; Reverse Transcriptase Polymerase Chain Reaction; Role; Running; Sampling; Schools; Standards of Weights and Measures; Technical Expertise; Techniques; Testing; Time; Vision; Work; animal facility; animal resource; bacterial vector; design; experience; interest; investigator training; mutant; plasmid DNA; protein expression; restriction enzyme; vector

The Molecular Biology (MB) Module has been in effect since the first funding period of the Case VSRC P30 grant in 1997, has been stable in personnel, with Dr. Brady-Kalnay as Director, and from 1998 until July, 2006, and Carol Luckey as Manager. In the last funding period, the Module has worked especially closely with the Specialized Animal Resource Module to genotype over 45 mouse strains. Taken together with the previous functions of this Module, and with the acquisition of the ABI Prism 7000 qPCR has also become part of this Module (from the Bioinformatics module), we anticipate that the Module will be very heavily utilized. . . The MB Module will be located in centralized VSRC facilities in the Institute of Pathology, in close proximity to the vision investigators in the School (Appendix D). The equipment is listed in form HH, and is located as shown in the adjacent diagram. The Module Manager designed the space to maximize efficient use of the major equipment, including the thermocycler and qPCR. The MB Module is responsible for providing skilled technical expertise, equipment and techniques for PCR analysis/genotyping, quantification and imaging of gels, cloning, generating bacterial strains and the central storage of bacteria, vectors and cDNA constructs.

分子生物学（MB）模块自2009年Case VSRC P30赠款的第一个资助期以来一直有效。 1997年，人员稳定，由Brady-Kalnay博士担任主任，1998年至2006年7月，Carol勒基 作为经理。在上一个资助期间，该模块与专业动物资源中心密切合作， 对超过45种小鼠品系进行基因分型的模块。结合本模块的先前功能以及 ABI Prism 7000 qPCR的获得也成为该模块的一部分（来自生物信息学模块），我们 预计该模块将被大量使用。. . MB模块将位于病理研究所的集中VSRC设施内，靠近 在学校的视觉研究人员（附录D）。该设备列于表格HH中，其位置如 邻图模块经理设计的空间，以最大限度地有效利用主要设备，包括 热循环仪和qPCR。MB模块负责 提供熟练的技术专长、设备和技术， PCR分析/基因分型、凝胶定量和成像， 克隆，产生细菌菌株和中央储存 细菌、载体和cDNA构建体。