Core--Imaging and Histology

核心——影像与组织学

基本信息

  • 批准号:
    7410008
  • 负责人:
  • 金额:
    $ 15.69万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2007
  • 资助国家:
    美国
  • 起止时间:
    2007-04-01 至 2009-03-31
  • 项目状态:
    已结题

项目摘要

Gene therapy as a discipline cuts across the entire spectrum of biomedical research, and a successful program requires access to all of the technical resources available to modern biology. As one of these technologies, biological imaging, including histology, has made major contributions to our biomedical knowledge in recent years as the instrumentation and techniques available have become not only more sensitive, more diverse, and more robust, but also easier to use. We have developed within the past year the wholly new Michael Hooker Microscopy Facility which will allow the Imaging & Histology Core to offer to the UNC gene therapy research community a suite of state-of-the-art technologies and technical assistance to aid their work. UNC's gene therapy research focuses on an expansion of knowledge of molecular mechanisms involved with the delivery and permanent expression of the therapeutic transgenes, with the long range objective of providing novel therapeutic modalities for treating monogenetic diseases such as cystic fibrosis and hemophilia. From the experience of two controlled clinical trials, the UNC Program has established the following objectives, 1) translational research with defined clinical endpoints, that provide a basic understanding of efficient gene delivery to airway epithelia for CF and liver cells for hemophilia; 2) the development of high titer viral vectors that offer safe, efficient long-term transgene expression; and 3), the development of novel animal models to help us better understand rate limiting steps in target cell transduction. The first and last of these requires heavy utilization of imaging and histology which the Imaging & Histology Core will serve with specific aims pertinent to the provision of [i] digital based widefield and confocal microscopy and cryo-cooled CCD-based luminometry, [ii] electron microscopy (including freeze fracture/freeze etch), [iii] tissue processing and sectioning for frozen specimens, or embedding in wax or plastic as appropriate, for light microscopy, and [iv] tissue processing and ultrathin sectioning services for embedding in plastic as appropriate to transmission electron microscopy. The Core will also assist investigators with the development of techniques for the expression and visualization of probes within living specimens or of novel or improved histological methodologies, and Core staff will consult with gene therapy investigators on methods of image quantitation.
基因治疗作为一门学科横跨了整个生物医学研究领域,一个成功的项目需要获得现代生物学可用的所有技术资源。作为这些技术之一,生物成像,包括组织学,近年来对我们的生物医学知识做出了重大贡献,因为可用的仪器和技术不仅变得更敏感,更多样化,更强大,而且更容易使用。在过去的一年里,我们开发了全新的Michael Hooker显微镜设备,这将使成像和组织学核心能够为北卡罗来纳大学基因治疗研究社区提供一套最先进的技术和技术援助,以帮助他们的工作。北卡罗来纳大学的基因治疗研究侧重于分子生物学知识的扩展

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

C. William Davis其他文献

Role of MARCKS in regulated secretion from mast cells and airway goblet cells.
MARCKS 在肥大细胞和气道杯状细胞分泌调节中的作用。
Mechanosensitivity of mouse tracheal ciliary beat frequency: roles for Ca2+, purinergic signaling, tonicity, and viscosity.
小鼠气管纤毛搏动频率的机械敏感性:Ca2+、嘌呤能信号、张力和粘度的作用。

C. William Davis的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('C. William Davis', 18)}}的其他基金

Molecular Pathways Regulating Airway Goblet Cell Mucin Secretion
调节气道杯状细胞粘蛋白分泌的分子途径
  • 批准号:
    8217298
  • 财政年份:
    2010
  • 资助金额:
    $ 15.69万
  • 项目类别:
Molecular Pathways Regulating Airway Goblet Cell Mucin Secretion
调节气道杯状细胞粘蛋白分泌的分子途径
  • 批准号:
    7886020
  • 财政年份:
    2010
  • 资助金额:
    $ 15.69万
  • 项目类别:
Molecular Pathways Regulating Airway Goblet Cell Mucin Secretion
调节气道杯状细胞粘蛋白分泌的分子途径
  • 批准号:
    8049606
  • 财政年份:
    2010
  • 资助金额:
    $ 15.69万
  • 项目类别:
Molecular Pathways Regulating Airway Goblet Cell Mucin Secretion
调节气道杯状细胞粘蛋白分泌的分子途径
  • 批准号:
    8435548
  • 财政年份:
    2010
  • 资助金额:
    $ 15.69万
  • 项目类别:
Imaging and Histology Core
成像和组织学核心
  • 批准号:
    7688322
  • 财政年份:
    2009
  • 资助金额:
    $ 15.69万
  • 项目类别:
Core--Imaging and Histology
核心——影像与组织学
  • 批准号:
    6774596
  • 财政年份:
    2003
  • 资助金额:
    $ 15.69万
  • 项目类别:
Autocrine regulation of ciliary beat frequency
纤毛跳动频率的自分泌调节
  • 批准号:
    6576228
  • 财政年份:
    2002
  • 资助金额:
    $ 15.69万
  • 项目类别:
Regulation of Airway Goblet Cell Mucin Secretion
气道杯状细胞粘蛋白分泌的调节
  • 批准号:
    7027079
  • 财政年份:
    2000
  • 资助金额:
    $ 15.69万
  • 项目类别:
INTRACELLULAR PATHWAYS MEDIATING AIRWAY MUCIN SECRETION
介导气道粘蛋白分泌的细胞内途径
  • 批准号:
    6027990
  • 财政年份:
    2000
  • 资助金额:
    $ 15.69万
  • 项目类别:
Regulation of Airway Goblet Cell Mucin Secretion
气道杯状细胞粘蛋白分泌的调节
  • 批准号:
    6776225
  • 财政年份:
    2000
  • 资助金额:
    $ 15.69万
  • 项目类别:

相似海外基金

Quantification of Neurovasculature Changes in a Post-Hemorrhagic Stroke Animal-Model
出血性中风后动物模型中神经血管变化的量化
  • 批准号:
    495434
  • 财政年份:
    2023
  • 资助金额:
    $ 15.69万
  • 项目类别:
Small animal model for evaluating the impacts of cleft lip repairing scar on craniofacial growth and development
评价唇裂修复疤痕对颅面生长发育影响的小动物模型
  • 批准号:
    10642519
  • 财政年份:
    2023
  • 资助金额:
    $ 15.69万
  • 项目类别:
Bioactive Injectable Cell Scaffold for Meniscus Injury Repair in a Large Animal Model
用于大型动物模型半月板损伤修复的生物活性可注射细胞支架
  • 批准号:
    10586596
  • 财政年份:
    2023
  • 资助金额:
    $ 15.69万
  • 项目类别:
A Comparison of Treatment Strategies for Recovery of Swallow and Swallow-Respiratory Coupling Following a Prolonged Liquid Diet in a Young Animal Model
幼年动物模型中长期流质饮食后吞咽恢复和吞咽呼吸耦合治疗策略的比较
  • 批准号:
    10590479
  • 财政年份:
    2023
  • 资助金额:
    $ 15.69万
  • 项目类别:
Diurnal grass rats as a novel animal model of seasonal affective disorder
昼夜草鼠作为季节性情感障碍的新型动物模型
  • 批准号:
    23K06011
  • 财政年份:
    2023
  • 资助金额:
    $ 15.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Longitudinal Ocular Changes in Naturally Occurring Glaucoma Animal Model
自然发生的青光眼动物模型的纵向眼部变化
  • 批准号:
    10682117
  • 财政年份:
    2023
  • 资助金额:
    $ 15.69万
  • 项目类别:
A whole animal model for investigation of ingested nanoplastic mixtures and effects on genomic integrity and health
用于研究摄入的纳米塑料混合物及其对基因组完整性和健康影响的整体动物模型
  • 批准号:
    10708517
  • 财政年份:
    2023
  • 资助金额:
    $ 15.69万
  • 项目类别:
A Novel Large Animal Model for Studying the Developmental Potential and Function of LGR5 Stem Cells in Vivo and in Vitro
用于研究 LGR5 干细胞体内外发育潜力和功能的新型大型动物模型
  • 批准号:
    10575566
  • 财政年份:
    2023
  • 资助金额:
    $ 15.69万
  • 项目类别:
Elucidating the pathogenesis of a novel animal model mimicking chronic entrapment neuropathy
阐明模拟慢性卡压性神经病的新型动物模型的发病机制
  • 批准号:
    23K15696
  • 财政年份:
    2023
  • 资助金额:
    $ 15.69万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
The effect of anti-oxidant on swallowing function in an animal model of dysphagia
抗氧化剂对吞咽困难动物模型吞咽功能的影响
  • 批准号:
    23K15867
  • 财政年份:
    2023
  • 资助金额:
    $ 15.69万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了