PROTEOMICS MODULE

完成了大量生物体的基因组测序， 包括人类、老鼠和大鼠在内的新技术的出现 质谱学，以及计算工具的开发 质谱学数据与基因组/蛋白质序列数据库 蛋白质的鉴定和共价结构分析 为大规模研究蛋白质创造了前所未有的机会 比例。蛋白质组学是对蛋白质表达的大规模研究， 蛋白质结构-功能和蛋白质-蛋白质相互作用及其利用 基因组数据库，以了解蛋白质如何在生物中发挥作用 细胞。作为利用先进的分子生物学技术 重组DNA技术极大地促进了 了解生物过程背后的分子机制， 蛋白质组学的新兴技术有望成为推动 未来的生物医学科学。 凯斯西储大学已经配备了研究 进行蛋白质组学研究的资源，包括最先进的质量 蛋白质分离系统光谱仪(多维高效液相色谱仪 和2D-PAGE)和生物信息学工具 蛋白质组学，由马克·钱斯博士指导。过去的VSRC案例 一年与蛋白质组学中心建立了合作伙伴关系 有效地为视觉科学家提供资源，并建议 通过更新P30核心补助金来加强这一关系。这个 单元主任，Krzysztof Palczewski博士，教授兼 药理学系及其联席主任宫城正郎博士， 蛋白质组学、眼科学中心助理教授 药理学，两位视觉科学家都将领导这一努力。他们在一起 将成为VSRC和蛋白质组学中心之间的桥梁 促进凯斯大学视觉科学家使用蛋白质组学方法。 由于帕尔谢夫斯基博士去年的招聘，对蛋白质组学支持的需求不断增加，包括最近的 招聘另一位国家公认的视觉科学家、蛋白质组学前主任西奥多·温塞尔博士 贝勒模块)，拒绝使用生物信息学模块的大部分功能，以及形成 凯斯蛋白质组学中心，2005年秋季用该模块的预算建立了一个蛋白质组学模块 由前生物信息学模块以前的预算提供支助。蛋白质组学模块实验室将与 2006年秋季VSRC 1楼统一病理研究所空间的其他模块(见下文 绘图)。 随着帕尔切夫斯基博士的招聘，帕尔切夫斯基博士已经在这一领域开展了重要的项目，该学院 在马克·钱斯博士的指导下创建了蛋白质组学中心。该设施(5000平方英尺)FT)位于9日 BRB大楼的楼层。该中心的设备在下面的资源部分中介绍。中心设施 从蛋白质组学模块实验室的当前和未来位置步行5分钟即可到达 走廊和/或隧道。蛋白质组学模块设施配备了实验室工作台、橱柜、中央空调、燃气 以及真空接线器、细胞培养室、暗室、步入冷藏室和生化实验室。

The completion of the genome sequencing of a multitude of organisms, including human, mouse and rat, the emergence of new technologies in mass spectrometry, and the development of computational tools that link mass spectrometry data with genome/protein sequence databases for the identification and covalent structural analysis of proteins have together fostered unprecedented opportunities for studying proteins on a large scale. Proteomics is the large-scale study of the protein expressions, protein structure-functions and protein-protein interactions, and utilizing genome databases in order to understand how proteins work in the living cells. As sophisticated molecular biological techniques utilizing recombinant DNA techniques have contributed greatly to the understanding of molecular mechanisms underlying biological processes, the emerging techniques of proteomics are expected to be driving forces of the future biomedical sciences. Case Western Reserve University is already equipped with research resources to carry out proteomic studies, including state-of-the-art mass spectrometers, protein separation systems (both multi-dimensional HPLC and 2D-PAGE) and bioinformatics tools within the Center for Proteomics, directed by Dr. Mark Chance. The Case VSRC in the past year has formed a partnership with the Center for Proteomics to utilize the resources effectively for the vision scientists and proposes to strengthen this relationship with the renewal of the P30 Core Grant. The Module Director, Dr. Krzysztof Palczewski, Professor and Chair of the Department of Pharmacology, and its co-director, Dr. Masaru Miyagi, Assistant Professor in the Center for Proteomics, Ophthalmology, and Pharmacology, both vision scientists will lead this effort. Together they will be a bridge between the VSRC and the Center for Proteomics to facilitate the use of proteomic approaches for the vision scientists at Case. As a result of Dr. Palczewski's recruitment last year, increasing demand for proteomics support including the recent recruitment of Dr. Theodore Wensel, another nationally recognized vision scientist and former Director of the Proteomics Module at Baylor), declining use of the majority of functions of the Bioinformatics Module, and the formation of the Center for Proteomics by Case, a Proteomics Module was constituted in the fall of 2005 with the budget of this Module supported by the previous budget of the former Bioinformatics Module. The Proteomics Module laboratory will be with the other Modules in the unified Institute of Pathology space for the VSRC on the 1st floor in the fall of 2006 (see adjacent drawing). Along with the recruitment of Dr. Palczewski who already had a significant program in this area, the School has created the Center for Proteomics under the direction of Dr. Mark Chance. The facility (5,000 sq. ft) is located on the 9th floor of the BRB Building. The equipment of this center is described in the Resource section below. The Center facilities are within 5 minutes walking distance from the current and future location of the Proteomics Module lab space by hallways and/or tunnel. The Proteomics Module facility is equipped with laboratory benches, cabinetry, central air, gas and vacuum hook-ups, cell culture room, dark room walk-in cold room and biochemistry laboratory.