PROTEOMICS MODULE

蛋白质组学模块

• 批准号: 7286549
• 负责人: KRZYSZTOF PALCZEWSKI
• 金额: $ 5.3万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7286549
• 关键词: Address; Affinity Chromatography; Air; Amino Acid Sequence Databases; Antibodies; Area; Arts; Biochemistry; Bioinformatics; Biological; Biological Process; Budgets; Cancer Center Support Grant; Categories; Cell physiology; Cells; Chemical Structure; Chemicals; Complex; Cultured Cells; Daily; Data; Databases; Development; Disease; Disease Progression; Emerging Technologies; Equipment; Floor; Fostering; Funding; Future; Gases; Gel; Genome; Goals; High Pressure Liquid Chromatography; Human; Image Analysis; Immunoprecipitation; Institutes; Investigation; Isoelectric Point; Isotope Labeling; Label; Laboratories; Lead; Life; Life Cycle Stages; Link; Location; Mass Spectrum Analysis; Membrane Proteins; Metals; Methods; Modification; Modification Type; Molecular; Molecular Structure; Mus; Numbers; Ophthalmology; Organism; Pathology; Pathway interactions; Peptides; Pharmaceutical Preparations; Pharmacology; Phosphorylation; Post-Translational Protein Processing; Process; Protein Analysis; Protein Isoforms; Protein translocation; Proteins; Proteome; Proteomics; Protocols documentation; Rattus; Recombinant DNA; Research Personnel; Resources; Sampling; Schools; Science; Scientist; Set protein; Signal Transduction; Site; Spottings; Stable Isotope Labeling; Standards of Weights and Measures; Stimulus; Structural Protein; Structure; System; Technical Expertise; Techniques; Testing; Tissues; Two-Dimensional Gel Electrophoresis; Universities; Vacuum; Vertebral column; Vision; Vision research; Walking; Work; base; computerized tools; driving force; falls; genome database; genome sequencing; glycosylation; interest; mass spectrometer; new technology; professor; programs; protein expression; protein protein interaction; protein structure function; ranpirnase; research study; size; tandem mass spectrometry; tool

The completion of the genome sequencing of a multitude of organisms, including human, mouse and rat, the emergence of new technologies in mass spectrometry, and the development of computational tools that link mass spectrometry data with genome/protein sequence databases for the identification and covalent structural analysis of proteins have together fostered unprecedented opportunities for studying proteins on a large scale. Proteomics is the large-scale study of the protein expressions, protein structure-functions and protein-protein interactions, and utilizing genome databases in order to understand how proteins work in the living cells. As sophisticated molecular biological techniques utilizing recombinant DNA techniques have contributed greatly to the understanding of molecular mechanisms underlying biological processes, the emerging techniques of proteomics are expected to be driving forces of the future biomedical sciences. Case Western Reserve University is already equipped with research resources to carry out proteomic studies, including state-of-the-art mass spectrometers, protein separation systems (both multi-dimensional HPLC and 2D-PAGE) and bioinformatics tools within the Center for Proteomics, directed by Dr. Mark Chance. The Case VSRC in the past year has formed a partnership with the Center for Proteomics to utilize the resources effectively for the vision scientists and proposes to strengthen this relationship with the renewal of the P30 Core Grant. The Module Director, Dr. Krzysztof Palczewski, Professor and Chair of the Department of Pharmacology, and its co-director, Dr. Masaru Miyagi, Assistant Professor in the Center for Proteomics, Ophthalmology, and Pharmacology, both vision scientists will lead this effort. Together they will be a bridge between the VSRC and the Center for Proteomics to facilitate the use of proteomic approaches for the vision scientists at Case. As a result of Dr. Palczewski's recruitment last year, increasing demand for proteomics support including the recent recruitment of Dr. Theodore Wensel, another nationally recognized vision scientist and former Director of the Proteomics Module at Baylor), declining use of the majority of functions of the Bioinformatics Module, and the formation of the Center for Proteomics by Case, a Proteomics Module was constituted in the fall of 2005 with the budget of this Module supported by the previous budget of the former Bioinformatics Module. The Proteomics Module laboratory will be with the other Modules in the unified Institute of Pathology space for the VSRC on the 1st floor in the fall of 2006 (see adjacent drawing). Along with the recruitment of Dr. Palczewski who already had a significant program in this area, the School has created the Center for Proteomics under the direction of Dr. Mark Chance. The facility (5,000 sq. ft) is located on the 9th floor of the BRB Building. The equipment of this center is described in the Resource section below. The Center facilities are within 5 minutes walking distance from the current and future location of the Proteomics Module lab space by hallways and/or tunnel. The Proteomics Module facility is equipped with laboratory benches, cabinetry, central air, gas and vacuum hook-ups, cell culture room, dark room walk-in cold room and biochemistry laboratory.

完成了大量生物体的基因组测序， 包括人类、小鼠和大鼠， 质谱，以及计算工具的发展， 质谱数据与基因组/蛋白质序列数据库， 蛋白质鉴定和共价结构分析 为研究蛋白质提供了前所未有的机会， 规模蛋白质组学是对蛋白质表达的大规模研究， 蛋白质结构-功能和蛋白质-蛋白质相互作用， 基因组数据库，以了解蛋白质如何在生活中工作， 细胞复杂的分子生物学技术利用 重组DNA技术为人类的 了解生物过程的分子机制， 新兴的蛋白质组学技术有望成为 未来的生物医学科学。 凯斯西储大学已经具备研究能力 进行蛋白质组学研究的资源，包括最先进的质量 光谱仪、蛋白质分离系统（包括多维HPLC 和2D-PAGE）和生物信息学工具， 蛋白质组学，由Mark Chance博士指导。案例VSRC在过去 一年来，我们与蛋白质组学中心建立了合作伙伴关系， 有效地为视觉科学家提供资源，并建议 通过更新P30核心补助金加强这种关系。的 模块主任，Krzysztof Palczewski博士，教授和主席 药理学系主任宫城博士， 助理教授在中心蛋白质组学，眼科，和 药理学，两位视觉科学家将领导这项工作。他们一起 将成为VSRC和蛋白质组学中心之间的桥梁， 促进凯斯视觉科学家使用蛋白质组学方法。 由于Palczewski博士去年的招聘，对蛋白质组学支持的需求不断增加，包括最近的 招募西奥多·温塞尔博士，另一位全国公认的视觉科学家和前蛋白质组学主任 模块），生物信息学模块的大部分功能的使用下降，以及 2005年秋季，利用蛋白质组学模块的预算，成立了蛋白质组学模块 由前生物信息学模块的先前预算支持。蛋白质组学模块实验室将与 2006年秋季，VSRC在一楼的统一病理学研究所空间的其他模块（见附件 绘图）。 沿着博士的招聘. Palczewski谁已经在这方面有一个重要的计划，学校已经 在马克·钱斯博士的指导下创建了蛋白质组学中心。该设施（5,000平方米）ft）位于9日 BRB大楼的一楼。该中心的设备在下面的资源部分中描述。中心设施 距离蛋白质组学模块实验室空间的当前和未来位置不到5分钟步行距离， 走廊或隧道。蛋白质组学模块设施配备了实验室工作台，橱柜，中央空调，气体 和真空连接装置、细胞培养室、暗室、步入式冷藏室和生物化学实验室。