Cell Biology Core

14)细胞生物学和成像核心(Gerace.导演：安布鲁斯-艾克林。经理) 概述 细胞生物学和成像核心提供对最先进的设施和光技术的访问 以及固定细胞和活细胞中大分子的电子显微镜成像，蛋白质组学分析 蛋白质的复杂混合物，以及不同体内外对细胞结构和功能的分析 技巧。专题重点和技术专长包括蛋白质的核质运输和 核蛋白复合体，核层等细胞多蛋白复合体的动力学，以及 用基于细胞的分析方法筛选核转运的小分子抑制物。的基础设施 该中心包括与斯克里普斯研究中心的Gaudenz Danuser博士和John Yates博士的合作 研究所。Gaudenz Danuser是数学分析和计算建模方面的领先专家 蜂窝组件的动态行为，并提供对旋转圆盘的仪器的访问 用于定量实时光学显微镜的共聚焦显微镜。John Yates提供对高吞吐量的访问 蛋白质组学用于复杂蛋白质组分的分析。

14) Cell Biology and Imaging Core (Gerace. Director: Ambrus-Aikelin. Manager) Overview The Cell Biology and Imaging Core provides access to state-of-the-art facilities and technologies for light and electron microscope imaging of macromolecules in fixed and living cells, proteomic analysis of complex mixtures of proteins, and analysis of cell structure and function by various in vitro and in vivo techniques. Thematic focus and technical expertise includes nucleocytoplasmic transport of proteins and nucleoprotein complexes, dynamics of multiprotein complexes of cells such as the nuclear lamina, and screening for small molecule inhibitors of nuclear transport using cell-based assays. The infrastructure of the center includes collaborations with Drs. Gaudenz Danuser and John Yates at the Scripps Research Institute. Gaudenz Danuser is a leading expert in mathematical analysis and computational modeling of the dynamic behavior of cellular components, and provides access to instrumentation for spinning disc confocal microscopy for quantitative real-time light microscopy. John Yates provides access to highthroughput proteomics for analysis of complex protein fractions.