IDENTIFICATION OF DDX1 AND REV/RRE COFACTORS

DDX1 和 REV/RRE 辅因子的鉴定

• 批准号: 8171227
• 负责人: Larry R. Gerace
• 金额: $ 0.48万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171227
• 关键词: Binding; Biological Assay; Cell Nucleus; Cell membrane; Cells; Computer Retrieval of Information on Scientific Projects Database; DDX1 gene; Data; Funding; Gene Expression; Genome; Grant; HIV; HIV-1; Hela Cells; Institution; Nuclear; Nuclear Export; Phase; Proteins; RNA Helicase; RNA Splicing; Recruitment Activity; Research; Research Personnel; Resources; Response Elements; Role; Series; Source; Technology; Transcript; Translations; United States National Institutes of Health; Viral; Viral Genes; Virus; mRNA Export; receptor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Human immunodeficiency virus (HIV)-1 Rev is essential in the late phase of viral gene expression and replication. Rev binds to the Rev response element (RRE) that occurs in unspliced and singly spliced HIV mRNAs, and recruits the nuclear export receptor CRM1 to these transcripts to facilitate their transport out of the nucleus. In addition to its functions in viral mRNA export, Rev also has been suggested to stabilize and regulate translation of RRE-containing mRNAs, and potentially has a role in targeting the HIV genome to the plasma membrane for encapsulation in the budding virus. Rev-interacting host cell proteins that are required for Rev/RRE functions include the RNA helicases DDX1 and DDX3.Our aim is to identify DDX1 and Rev/RRE binding partners from nuclear and cytosolic extracts of HeLa cells by a series of pull-down assays, and subsequent analysis by multidimensional protein identification technology (MudPIT) followed by stringent data filtering.

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