ANALYTIC SERVICES

分析服务

• 批准号: 7493854
• 负责人: CLEMENCIA COLMENARES
• 金额: $ 9.9万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7493854
• 关键词: Amino Acid Sequence; Biological Markers; Blood Platelets; CD36 gene; Cells; Computers; Computers and Advanced Instrumentation; Consultations; Data Analyses; Detection; Endothelial Cells; Ensure; Experimental Designs; Flow Cytometry; Informatics; Laboratories; Leukocytes; Mass Spectrum Analysis; Mus; Patients; Peptide Sequence Determination; Peptides; Phospholipids; Plasma; Procedures; Protein Analysis; Range; Research Institute; Research Personnel; Resources; Sampling; Services; Staining method; Stains; Surface; Technology; analytical method; human subject; instrument; programs; small molecule

The overall objective of the Analytic Core E is to provide SCCOR project investigators with all of the technology, expertise, and advanced instrumentation needed to perform Mass Spectrometry and Flow Cytometry analyses. The Analytic Core will take advantage of much of the existing network of scientific support services already existing within the Lerner Research Institute, and will leverage the resources requested to ensure that these services are easily accessible to the SCCOR projects in a timely and consistent manner. The services and resources described below will include Flow Cytometry, Mass Spectrometry, and associated informatic and computer support. The Flow Cytometry section will provide investigators with a resource for analytical and preparative studies of cells using flow cytometry. The core offer access to several different Flow Cytometry instruments, as well as a broad range of services from consultation, experimental design, and data analysis to staining of cells for specific procedures. Specific expertise of the core will be provided to study quantitative expression of platelet surface CD36; assessment of platelet-leukocyte conjugates; identification and characterization of platelet, endothelial and leukocyte microparticles in samples from human subjects and mice; and assessment of platelet leukocyte and endothelial cell activation. The Mass Spectrometry section of the Core will provide SCCOR investigators with access to advanced instrumentation and expertise for the analysis of proteins, peptides, oxidized phospholipids, and small biomarkers. The laboratories have 2 components: a proteornics program characterized by high-throughput protein sequencing and identification; and a small molecule analysis program that offers investigators the ability to develop and use analytical methods for detection, quantification, and isolation of biomarkers in plasma and in atheromatours materials isolated from patient samples.

分析核心E的总体目标是为SCCOR项目调查人员提供所有 进行质谱和流程所需的技术，专业知识和高级仪器 细胞仪分析。分析核心将利用现有的科学网络 支持服务已经存在于Lerner Research Institute中，并将利用资源 要求及时确保SCCOR项目易于访问这些服务 一致的方式。下面描述的服务和资源将包括流式细胞仪，质量 光谱法，以及相关的信息和计算机支持。流式细胞仪部分将提供 使用流式细胞术对细胞进行分析和制备研究的资源进行了研究。核心 提供几种不同的流式细胞仪仪器，以及从 咨询，实验设计和数据分析，以染色以进行特定程序。具体的 将提供核心的专业知识来研究血小板表面CD36的定量表达；评估 血小板 - 白细胞缀合物；血小板，内皮和白细胞的识别和表征 来自人类受试者和小鼠的样品中的微粒；和血小板白细胞和 内皮细胞激活。核心的质谱部分将为SCCOR研究者提供 有了获得高级仪器和专业知识，用于分析蛋白质，肽，氧化 磷脂和小生物标志物。实验室有2个组件：一个蛋白质计划 以高通量蛋白测序和鉴定为特征；和小分子分析 为调查人员提供开发和使用分析方法检测能力的计划， 定量和分离血浆中的生物标志物和从患者分离的动脉瘤材料中 样品。