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CORE--CELL AND IMAGING

核心——细胞与成像

基本信息

批准号：
7413751
负责人：
ANIRBAN BANERJEE
金额：
$ 34.82万
依托单位：
UNIVERSITY OF COLORADO DENVER
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7413751
关键词：
3-Dimensional Abbreviations Acetone Acute Adherence Adrenergic Agents Adult Affect Age Age-Years Air Algorithms Ally Amphotericin Animal Model Animals Antibiotics Antibodies Apple Applications Grants Archives Area Argon Arts Asses Atomic Force Microscopy Automation B-Lymphocytes Behavior Binding Biochemical Biochemistry Biological Assay Biology Biopsy Biotechnology Bisbenzimide Bite Blood Blood Vessels Books Boxing Buffers CD11 Antigens Caliber Carbon Dioxide Cell Line Cell Nucleus Cell surface Cells Centrifugation Characteristics Chemicals Class Clathrin Clinical Clinical Research Code Collaborations Color Colorado Compatible Complement Complex Computer software Computers Condition Confocal Microscopy Consent Contracts Cryoultramicrotomy Cultured Cells Custom Cyclophosphamide/Fluorouracil/Prednisone Cytoskeleton Data Data Storage and Retrieval Databases Depth Detection Development Digital Signal Processing Distant Doctor of Medicine Doctor of Philosophy Documentation Donor person Dyes Dynamin Eagles Elastases Elements Employee Employee Strikes Endocrine Endocytic Vesicle Endosomes Endothelial Cells Ensure Enzymes Epitopes Equipment Equipment and supply inventories Ethnic Origin Event Extramural Activities F-Actin Ficoll Flow Cytometry Fluorescein Fluorescein-5-isothiocyanate Fluoresceins Fluorescence Fluorescence Resonance Energy Transfer Foundations Fractals Freezing Functional disorder Funding Future Gender Generations Genus Capra Glass Goals Goat Grant Green Fluorescent Proteins Growth Factor HEPES HMGB Proteins HMGB1 gene Hand Harvest Health Heart Heating Helium Hormones Hospitals Hour Human Human Cell Line Human Resources Human Volunteers Hybrids Hypaque Image Image Analysis Image Cytometry Immersion Investigative Technique Immunoblotting Immunofluorescence Immunologic Immunoglobulin G Immunohistochemistry In Situ Hybridization Incubated Incubators Individual Inflammation Inflammation Mediators Injury Instruction Intention Intracellular Second Messenger Ions Island Krypton L-Selectin Label Laboratories Lasers Leadership Learning Length Leukocytes Libraries Life Light Link Lipids Literature Liver Localized Location Lung Lymph Masks Measurement Measures Mechanics Medial Melanocytic nevus Memory Mesentery Messenger RNA Methanol Methodology Methods Microscope Microscopy Mitochondria Modeling Mole the mammal Molecular Molecular Probes Monitor Morphologic artifacts Morphology Motor Mus NF-kappa B Names National Institute of General Medical Sciences Nature Neon Oils Operative Surgical Procedures Optics Oregon Organ Organ Donor Organ Transplantation Orthopedics Oryctolagus cuniculus Output Pancreas Pancreatic Elastase Pathologist Patients Penicillin G Perfusion Personal Satisfaction Personnel Staffing Persons Pharmaceutical Preparations Phase Photobleaching Physiology Positioning Attribute Postdoctoral Fellow Preparation Principal Investigator Procedures Process Productivity Progress Reports Protein Isoforms Proteins Protocols documentation Protons Publications Publishing Pump Purpose Quartz Range Rate Rattus Reagent Records Recovery Recruitment Activity Refractive Indices Reporting Research Research Ethics Committees Research Personnel Resolution Resources Rhodamine Rhodamines Role Route Rubber Running SHFM1 gene Saline Sample Size Sampling Screening procedure Second Messenger Systems Security Sepsis Series Serum Services Shapes Side Signal Transduction Site Slide Smooth Muscle Smooth Muscle Actin Staining Method Smooth Muscle Myocytes Solutions Spottings Staging Staining method Stains Standards of Weights and Measures Streptomycin Sulfate (2[{..}]3) Salt Surface Surface Antigens System T-Lymphocyte TLR2 gene TLR4 gene Techniques Technology Testing Therapeutic Thick Thoracic aorta Three-Dimensional Image Three-Dimensional Imaging Time Tissue Preservation Tissues Training Tranexamic Acid Transformed Cell Line Transfusion Trauma Trauma Research Trypsin Tube Tubulin Tumor Necrosis Factor Receptor United States National Institutes of Health Universities Update Vascular Endothelial Cell Vascular Smooth Muscle Vendor Vesicle Water Week Work Xenon adrenergic analytical method autocrine base blood lipid caN protocol cell fixing cell type cellular imaging charge coupled device camera collagenase cost cyanine dye 5 cytokine data acquisition day density design desire detector digital digital imaging evaporation experience falls feeding fetal flasks fluorophore follower of religion Jewish human subject human tissue image processing immunological synapse improved injured innovation insight instrument instrumentation intercellular cell adhesion molecule interest interstitial lens light emission lipofection macrophage member milligram millisecond molecular recognition nano neutrophil novel optical imaging paracrine paraform photomultiplier pressure programs radius bone structure receptor expression receptor for advanced glycation endproducts reconstruction repaired research study response sample fixation size skills solid state statistics structural biology submicron success tissue culture tool trafficking trauma centers uptake user-friendly volunteer

项目摘要

This core will coordinate and provide: 1. human volunteer PMNs; 2. batches of human endothelial cell lines (pulmonary microvascular hMVEC); 3. imaging and optical experiment facility (FRET, 3D reconstruction, multichannel imaging and cytometry); and 4. efficient use of a perishable inventory of labeled primary and secondary antibodies. At present the Core is equipped with two digital deconvolving microscopes and Beckman Coulter flow cytometer and full tissue culture capabilities. Both scopes are equipped to perform live or fixed cell 3D imaging in upto 4 color channels. The Core is staffed by personnel who are highly experienced in all aspects of primary human cell culture and various imaging methodologies. The core will actively move into the future (2-4 years), 1. by linking data to national databases (primarily the GEO project) and 2. expanding its own repertoire of analytical methods before the need arises, 3. Linking to other core analytical efforts (NMR, MS, microarrays) within the TCG.

该核心将协调并提供：1.人志愿者PMNs; 2.人内皮细胞系（肺微血管hMVEC）的批次; 3.成像和光学实验设备（FRET、3D重建、多通道成像和细胞术）;和4.有效利用标记的一抗和二抗的易腐库存。目前，核心配备了两个数字去卷积显微镜和贝克曼库尔特流式细胞仪和完整的组织培养能力。这两款示波器都配备了多达4个颜色通道的活细胞或固定细胞3D成像。核心由在原代人类细胞培养和各种成像方法的各个方面都具有丰富经验的人员组成。核心将积极走向未来（2-4年），1。通过将数据与国家数据库（主要是全球环境展望项目）连接起来;在需要之前扩展自己的分析方法库，3.与TCG内的其他核心分析工作（NMR，MS，微阵列）相关联。