CORE--NEUROCHEMISTRY

神经化学核心将整合和巩固在以下几个基础上进行的活动 该中心的实验室分成三个单位，在摩尔、库珀和贾维奇博士的指导下， 分别为： 1)体内和体外脑儿茶酚胺、吲哚胺和氨基酸的测定 发射机水平(摩尔)。该单位将为该中心在啮齿动物和灵长类动物体内使用微透析的项目(Laurelle、Kandel和Rayport的项目)提供后勤支持和技术专长，包括实验设计、实施以及体内脑透析液样本和体外脑组织匀浆中儿茶酚胺、吲哚胺和氨基酸的色谱分析(Laurelle、Javitt、Kandel和Rayport的项目)。 2)药物动力学单位(库珀)。该单位将开发和执行血浆水平的分析 用于小鼠(坎德尔和雷波特的项目)；非人类灵长类(劳雷勒和贾维特的项目)和人类(阿比-达勒姆和劳瑞尔的项目)的药理制剂(如MK801、NNC112、SCH 39166、苯丙胺、五氯苯酚、利培酮)。 3)受体结合单位(Javitch)。该单元将整合实验室中进行的活动 贾维奇、雷波特和黄的名字。第一个任务是进行体外结合分析，并表征由脑成像核心开发的候选放射性配体，以及Abi-Dargham和Laurelle项目的项目。第二个任务是研究受体贩运对D2和D1放射性示踪剂结合亲和力的影响。后一项研究将为使用内源性竞争技术的成像研究提供重要信息，如Laurelle在Project中使用的技术，并将有助于开发具有 对内源性递质，特别是多巴胺和谷氨酸的突触/细胞外浓度变化敏感的结合(见脑成像核心)。 总之，神经化学核心将共享人员，并将与Abi-Dargham、Laurelle、Javitt、Kandel&Rayport以及脑成像核心和细胞/分子核心的项目密切合作，以提供动物模型中神经递质谱的筛选和以假设为导向的表征，开发结合灵长类和啮齿类动物体内微透析和功能脑成像的新方法，并开发用于功能性脑成像的候选放射性配基的表征方法。

The Neurochemistry Core will integrate and consolidate the activities performed in several basic laboratories of this Center into three units, under the direction of Drs Moore, Cooper and Javitch, respectively: 1) In vivo and ex vivo determination of brain catecholamine, indoleamine and amino acid transmitter levels (Moore). This unit will provide logistical support and technical expertise to projects of the Center that employ in vivo microdialysis in rodents and primates (Projects by Laurelle, Kandel, Rayport) including experimental design, implementation, and chromatographic analysis of catecholamines, indolamines and amino acids in in vivo brain dialysate samples and ex vivo brain tissue homogenates (Projects by Laurelle, Javitt, Kandel, Rayport). 2) Pharmacokinetic Unit (Cooper). This unit will develop and perform analysis of plasma levels of pharmacological agents (e.g. MK 801, NNC 112, SCH 39166, amphetamine, PCP, risperidone) used in mice (Projects by Kandel, Rayport ); non-human primates (Projects by Laurelle and Project by Javitt) and humans (Projects by Abi-Dargham and Laurelle). 3) Receptor Binding Unit (Javitch). This unit will integrate activiiies performed in the laboratories of Javitch, Rayport, and Huang. The first mission is to perform in vitro binding assay and to characterize candidate radioligands developed by the Brain Imaging Core and Projects by Abi-Dargham and Project by Laurelle. The second mission is to study the impact of receptor trafficking on binding affinities of D2 and D1 radiotracers. The latter investigation will provide important information for imaging studies using endogenous competition techniques such as used in Project by Laurelle, and will contribute to the development of novel radioligands with binding that is sensitive to changes in synaptic/extracellular concentrations of endogenous transmitters, specifically dopamine and glutamate (see Brain Imaging Core). In summary, the Neurochemistry Core will share personnel and will work closely with Projects by Abi-Dargham, Laurelle, Javitt, Kandel & Rayport and the Brain Imaging Core and Cellular/Molecular Core to provide screening and hypothesis-directed characterization of neurotransmitter profiles in animal models, development of novel methods for combining in vivo microdialysis and functional brain imaging in primates and rodents, and development of methods for characterization of candidate radioligands for functional brain imaging.