Centrifugal Precipitation Chromatography for Biotechnology

用于生物技术的离心沉淀色谱法

• 批准号: 7539991
• 负责人: Martha Knight
• 金额: $ 10万
• 依托单位: CC BIOTECH, LLC
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-15 至 2009-12-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7539991
• 关键词: Achievement; Address; Biotechnology; Cells; Centrifugation; Chimeric Proteins; Chromatography; Class; Commit; Condition; Cultured Cells; DNA; Diagnostic; Dialysis procedure; Doctor of Philosophy; Escherichia coli; Fermentation; Fiber; Filtration; Fractionation; Future; Goals; Healthcare; Industry; Label; Laboratory Research; Licensing; Marketing; Membrane; Methods; Molecular; Monitor; NIH Program Announcements; Nanotubes; Performance; Personal Satisfaction; Pharmaceutical Preparations; Phase; Physical Dialysis; Plasmids; Polymers; Precipitation; Process; Proteins; Proteomics; Protocols documentation; Public Health; Pump; Rate; Research; Sampling; Sodium Chloride; Solvents; Staging; Stream; System; Techniques; Technology; Time; Ubiquitin; United States National Institutes of Health; Vaccine Production; Vaccines; cost; design; improved; innovation; instrument; interest; nanomedicine; new technology; novel; novel therapeutics; point of care; present value; protein expression; prototype; scale up; seal; success; technology development

DESCRIPTION (provided by applicant): Despite great progress in cell culture technology to produce proteins and biologics in high yields from large volume fermentations, there is a bottleneck in the industry because of the lack of high throughput in the purification process. Centrifugal precipitation chromatography, a new method invented at the National Institutes of Health and licensed by CC Biotech LLC, offers unique capabilities for the purification of large molecules. In gradients of salt or solvents, discrete fractionation of large molecules is possible, aided by centrifugal forces. This method is well adapted to proteins, DNA and many bio-polymers. In a flow-through centrifuge, proteins are held by a filtration membrane as they are precipitated by the eluting agents. The process of precipitation and re-dissolving is repeated many times, causing the components to separate from each other at their different rates in the process. Special eluting conditions can be created for many types of molecules. This project is aimed at manufacturing the instrument for the research laboratory market such that its use will develop novel purification methods to simplify the process for biotechnology products and additionally, develop parameters for scaling up in the industry. PUBLIC HEALTH RELEVANCE: A new technique of purification is centrifugal precipitation chromatography which is especially suitable for the large bio-molecules. As a result of this research, purification processes for new therapeutic biologics will be simplified, and the costs lowered, to provide very pure drugs and vaccines for healthcare. New manufacturing techniques will be applied to make an improved instrument for introduction on the market.

描述(申请人提供)：尽管细胞培养技术有了很大的进步，可以从大容量发酵中高产量地生产蛋白质和生物制品，但由于提纯过程缺乏高通量，该行业存在一个瓶颈。离心沉淀层析法是美国国立卫生研究院发明并获得CC Biotech LLC许可的一种新方法，它为大分子的纯化提供了独特的能力。在盐或溶剂的梯度中，在离心力的辅助下，大分子的离散分级是可能的。该方法适用于蛋白质、DNA和许多生物聚合物。在流通式离心机中，当蛋白质被洗脱剂沉淀时，蛋白质被滤膜保持。沉淀和再溶解的过程重复多次，导致各组分在该过程中以不同的速度彼此分离。可以为许多类型的分子创造特殊的洗脱条件。该项目旨在为研究实验室市场制造该仪器，以便它的使用将开发新的纯化方法，以简化生物技术产品的工艺，并开发在行业中扩大规模的参数。 公共卫生意义：一种新的纯化技术是离心沉淀层析法，它特别适合于生物大分子。这项研究的结果是，新的治疗性生物制品的纯化过程将得到简化，成本将降低，从而为医疗保健提供非常纯粹的药物和疫苗。将应用新的制造技术来制造改进的仪器，以便推向市场。