Tissue, Cell and Tissue Culture Core

核心B是该项目的组织、细胞和组织培养核心。在过去14年的供资中，这一核心资源一直在满足所有项目的需求。Core B以前的工作仅限于为各种项目提供klg组织、新鲜分散的细胞和培养的细胞和组织，用于形态、功能和分子分析。在这个融资周期中，一个令人兴奋的新增加的核心是细胞冲浪。 本节详细介绍核心的现有职能和新职能。Kathleen Keef博士将负责核心的整体检查，就像她过去5年所做的那样。Tamas Ordog博士现在已被添加为该核心的联合主任，他将负责监督细胞分选活动。 博士Ordog是目前使用细胞分选纯化ICC的技术发展的核心人物。在先前的资助期间，工作集中在三种不同的动物模型上（即，狗、小鼠和豚鼠）。在这一新的应用中，主要的焦点集中在小鼠模型上，因为它作为人类运动模型具有重要的实用性，并且因为通过获得敲除和转基因小鼠而产生的巨大优势。只要有可能，还将使用在非阻塞性肿瘤手术期间和在非阻塞性肿瘤手术期间获得的从人结肠分离的细胞和组织重复实验。 部分病例取自胃旁路手术的人空肠。Keef博士已经发表了使用这两种人类肌肉的研究。总之，核心B是实现本计划项目中所述5个项目中设定的目标的基本要素。

Core B is the tissue, cell and tissue culture core for the program project. This core has continuously served the requirements of all projects for the past 14 years of funding. The previous efforts of Core B were confined to supplyklg tissue, freshly dispersed cells and cultured cells and tissues to the various projects for morphological, functional and molecular analyses. An exciting new addition to this core for this funding cycle is cell surfing. Details of both the ongoing and new functions of the core are described in this section. Dr. Kathleen Keef will be responsible for the overall dkection of the core as she has done for the past 5 years. Dr. Tamas Ordog has now been added as a co-director of this core and he will be responsible for overseeing the cell sorting activities. Dr. Ordog has been the central figure in ithe development of techniques currently used here for the purification of ICC using cell sorting. During the previous fundinlg period work focused upon three different animal models (i.e., dog, mouse and guinea-pig). in this new application the major focus has shined to the mouse model since it has significant utility as a model of human motility and because of the enormous advantages engendered through access to knockout and transgenic mice. Whenever possible experiments will also be repeated using cells and tissues isolated from human colon obtained during surgeries for non-obstructive neoplasms and in some cases human jejunum obtained from gastric bypass surgeries. Dr. Keef has published studies using both of these human muscles. In summary, Core B is an essential element to achieving the goals set out in the 5 projects described in this program project.