Tissue, Cell and Tissue Culture Core

核心B是计划项目的组织、细胞和组织培养核心。在过去14年的供资中，这一核心不断满足所有项目的要求。核心B以前的努力仅限于为各种项目提供组织、新分散的细胞和培养的细胞和组织，以进行形态、功能和分子分析。在这一融资周期的核心中，一个令人兴奋的新成员是手机冲浪。 本节详细介绍了核心的现行职能和新职能。凯瑟琳·基夫博士将负责核心的全面指导，就像她在过去5年所做的那样。塔马斯·奥达格博士现已被任命为该核心的联席主任，他将负责监督细胞分选活动。 在目前使用细胞分选技术提纯ICC的技术开发中，Ordoge博士一直是核心人物。在之前的基金期间，工作集中在三种不同的动物模型(即狗、小鼠和豚鼠)上。在这一新的应用中，主要焦点放在小鼠模型上，因为它作为人类运动模型具有重要的实用价值，而且因为通过获得基因敲除和转基因小鼠而产生的巨大优势。只要有可能，也将使用从非梗阻性肿瘤手术中获得的人结肠细胞和组织来重复实验。 有些人的空肠是从胃旁路手术中获得的。基夫博士发表了使用这两块人体肌肉的研究。总而言之，核心B是实现本计划项目中描述的5个项目所定目标的基本要素。

Core B is the tissue, cell and tissue culture core for the program project. This core has continuously served the requirements of all projects for the past 14 years of funding. The previous efforts of Core B were confined to supplyklg tissue, freshly dispersed cells and cultured cells and tissues to the various projects for morphological, functional and molecular analyses. An exciting new addition to this core for this funding cycle is cell surfing. Details of both the ongoing and new functions of the core are described in this section. Dr. Kathleen Keef will be responsible for the overall dkection of the core as she has done for the past 5 years. Dr. Tamas Ordog has now been added as a co-director of this core and he will be responsible for overseeing the cell sorting activities. Dr. Ordog has been the central figure in ithe development of techniques currently used here for the purification of ICC using cell sorting. During the previous fundinlg period work focused upon three different animal models (i.e., dog, mouse and guinea-pig). in this new application the major focus has shined to the mouse model since it has significant utility as a model of human motility and because of the enormous advantages engendered through access to knockout and transgenic mice. Whenever possible experiments will also be repeated using cells and tissues isolated from human colon obtained during surgeries for non-obstructive neoplasms and in some cases human jejunum obtained from gastric bypass surgeries. Dr. Keef has published studies using both of these human muscles. In summary, Core B is an essential element to achieving the goals set out in the 5 projects described in this program project.