Pancreas Cancer Biospecimens Repository

胰腺癌生物标本库（核心A）将为本P01的研究者提供访问权限， 正常和肿瘤人胰腺组织、区域淋巴结、肿瘤抽吸物和患者血液 患有癌前和癌性胰腺疾病。核心的职能将包括收集 组织、临床注释、组织的适当储存和分发给研究者。核心将扮演一个 在确保转化研究所需的足够数量的胰腺标本方面发挥重要作用。目前， 根据AZCC的GI孢子资助，将收集胰腺肿瘤标本和血液制品 在图森医学界。核心A的目的不是重复收集这些试剂， 而是扩大可用样本类型的数量和多样性。知道任何单一的 为了收集足够数量的胰腺癌研究标本，核心研究所已经招募了 八个独立的医疗中心合作处理胰腺疾病。这些机构将提供 另外的新鲜标本（癌性和非癌性组织、局部淋巴结的部分和血液 产品），将在核心A下收集，创建一个集中的不同胰腺组织库， 用于调查研究的生物样本。人胰腺组织标本（胰腺癌，正常 胰腺和癌前组织）将在异戊烷冷却的液体中立即速冻后收集 氮，也保存在RNAlater中”。手术时收集的部分区域淋巴结 胰腺探查将被快速冷冻或在培养基中提交以允许收获淋巴细胞。 此外，将采集每例患者的血液样本，并分离至外周血单核细胞（PBMC）中。 细胞和血清，其可用于分析。与GI SPORE研究人员合作， 将在共享数据库中注释患者人口统计学、环境风险、暴露 病史、家族史和病理资料。这一全面的数据库将与下列机构合作开发： 模式分析和计算生物学核心（核心B）。额外的血液样本和临床进展 在两年内，每六个月会收到一次报告。在适当的保密监督下， AZCC和参与机构的P01研究人员可通过互联网获取信息。 将根据NCI指南获得所有参与者的知情同意书，并获得每个参与者的批准。 将由胰腺科医师监督并优先考虑向研究者分发标本。 生物资源利用委员会，该委员会将在本核心服务项下组织。

The Pancreas Cancer Biospecimens Repository (Core A) will provide investigators of this P01 with access to normal and neoplastic human pancreatic tissue, regional lymph nodes, tumor aspirates and blood from patients suffering from pre-cancerous and cancerous pancreatic diseases. Functions of the Core will include collection of tissues, clinical annotations, proper storage and distribution of tissue to investigators. The Core will play an essential role in ensuring adequate numbers of pancreatic specimens for translational research studies. Currently, under the GI SPORE Grant of the AZCC, pancreatic tumor specimens and blood products are to be collected within the Tucson medical community. The intent of Core A is not to duplicate the collection of these reagents, but rather to extend the number and diversity of available specimen types. Knowing the limitations of any single institute to collect ample numbers of specimens for research in pancreatic cancer, the Core has enlisted the collaboration of eight separate medical centers dealing with pancreatic diseases. These institutions will provide additional fresh specimens (cancerous and non-cancerous tissue, portions of regional lymph nodes, and blood products) that will be collected under Core A, creating a centralized repository of diverse pancreatic tissues and biospecimens for investigational studies. Human pancreatic tissue specimens (pancreatic cancer, normal pancreas, and pre-cancerous tissue) will be collected after immediate snap freezing in isopentane-cooled liquid nitrogen and also preserved in RNAlater". Portions of the regional lymph nodes collected at the time of surgical pancreatic exploration will be snap frozen or submitted in medial to allow harvesting of lymphocytes. Additionally, blood samples will be collected from each patient and separated into peripheral blood mononuclear cells and serum, which may be used for analysis. In collaboration with the GI SPORE investigators, specimens will be annotated in a shared database with regard to patient demographics, environmental risk, exposure, medical and family history and pathologic data. This comprehensive database will be developed in concert with the Pattern Analysis and Computational Biology Core (Core B). Additional blood samples and clinical progress reports will be obtained at 6 monthly intervals for two years. With appropriate confidentiality monitoring, information will be accessible via the Internet to the P01 researchers at the AZCC and participating institutions. Informed consent will be obtained for all participants according to NCI guidelines and with the approval of each institution's IRB.Distribution of specimens to investigators will be overseen and prioritized by the Pancreatic Bioresource Utilization Committee, which will be organized under this Core service.