NEOANTIGENICITY OF RIBOFLAVIN AND LIGHT TREATED RBCS IN BABOONS

狒狒核黄素和光处理红细胞的新抗原性

• 批准号: 7957909
• 负责人: Krishna K Murthy
• 金额: $ 3.71万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-06 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957909
• 关键词: Antibody Formation; Autologous; Back; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Immune response; Institution; Kinetics; Label; Papio; Primates; Recovery; Research; Research Personnel; Resources; Riboflavin; Source; Technology; Ultraviolet Rays; United States National Institutes of Health; aluminum sulfate; pathogen

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study was conducted to determine whether an immune response would be elicited to RBCs treated with the Mirasol pathogen reduction technology (MPRT) using Riboflavin and UV light. The second objective was to assess the survival and recovery of MPRT treated RBCs in baboons. Autologous RBC's either untreated or Mirasol-treated were formulated with alum adjuvant and was injected into the respective baboon donors. No antibody response to RBCs was observed either in the untreated or MPRT treated baboons. Next, untreated and MPRT treated RBCs were labeled with CR51 and infused back into the donors. Survival and recovery kinetics were quite similar in the untreated and MPRT treated baboons.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 本研究旨在确定使用核黄素和紫外线进行 Mirasol 病原体减少技术 (MPRT) 处理的红细胞是否会引发免疫反应。 第二个目标是评估狒狒经 MPRT 治疗后红细胞的存活率和恢复情况。 将未经处理或经过 Mirasol 处理的自体红细胞与明矾佐剂一起配制并注射到各自的狒狒供体中。 在未经治疗或 MPRT 治疗的狒狒中均未观察到针对红细胞的抗体反应。 接下来，未经处理和 MPRT 处理的红细胞被 CR51 标记并输回供体体内。 未经治疗和 MPRT 治疗的狒狒的生存和恢复动力学非常相似。