Core B--Immunological Monitoring

免疫监测核心B是SCCOR补助金的一个非常重要的组成部分。该核心将协助所有四个科学项目，并将为其他核心提供必要的信息。该核心的具体目标是： 目的1：采集、分检和储存外周血单个核细胞、血浆和血清， 肌内膜活检组织标本。 目的2：根据临床和基础项目的实验方案，处理个体研究者所需的标本。 目标3：从每例患者外周血中建立人淋巴母细胞样细胞系（LCL），并为EBV体外研究提供细胞系。 目标4：开发，评估和执行体外监测试验，以确定免疫效应细胞的频率和活性，以支持基础和临床项目。 核心将向研究者提供从参与各种研究的儿科心脏和心肺移植受者中获得的标本。为了评估生物治疗的免疫调节作用和/或EBV载量升高的影响，将在由核心开发和验证的一组检测试剂盒中检测这些系列标本。这些测定将鉴定、定量和表征循环中的同种异体反应性T细胞。其他测定将测量和表征这些患者外周血中EBV特异性T细胞的免疫缺陷。开发此类测定及其在治疗过程中评估免疫功能的应用是核心的重要组成部分。芯会 还进行项目1-3所需的HLA DNA分型。

The Immunological Monitoring Core B is a very essential component of the SCCOR grant. This core will assist all four scientific projects and will provide the necessary information to the other cores. The Specific Aims of this core are: Aim 1: To procure, triage and store peripheral blood mononuclear cells, plasma and serum, and endomyocardial biopsy tissue specimens. Aim 2: To process the specimens as required by individual investigators for experimental protocols in the clinical and basic projects. Aim 3: To establish the human lymphoblastoid cell lines (LCL) from peripheral blood for each patient, and maintain the cell lines for EBV in vitro studies. Aim 4: To develop, evaluate and perform in vitro monitoring assays to determine the frequency and activity of immune effector cells in order to support basic and clinical projects. The core will make available to the investigators the specimens obtained from pediatric heart and heart-lung transplant recipients participating in the various studies. In order to assess the immune modulation effect of biotherapies and/or impact of elevation of EBV load, these serial specimens will be tested in a panel of assays developed and validated by the Core. These assays will identify, quantitate and characterize the allo-reactive T-cells in the circulation. Other assays will measure and characterize the immune defects of EBV-specific T cells in peripheral blood of these patients. The development of such assays and their application to assess of immune functions in the course of therapy are essential components of the Core. The Core will also perform HLA DNA typing required for projects 1-3.