SURFACTANT PROTEIN B EXPRESSION IN AVIAN LUNG

禽肺中表面活性蛋白 B 的表达

• 批准号: 7720249
• 负责人: SABRINA M BROUGHER
• 金额: $ 4.41万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720249
• 关键词: Age; Alveolar; Birds; Bronchioles; Cell physiology; Cells; Computer Retrieval of Information on Scientific Projects Database; Embryo; Funding; Gases; Goals; Grant; Human; Immune response; In Vitro; Inflammatory Response; Institution; Localized; Lung; Lung diseases; Mediating; Modeling; Proteins; Pulmonary Surfactant-Associated Protein A; Pulmonary Surfactant-Associated Protein B; Pulmonary Surfactant-Associated Protein D; Research; Research Personnel; Resources; Site; Source; Surface Tension; System; Tissues; United States National Institutes of Health; Up-Regulation; interest; novel; prevent; surfactant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sabrina McGary (Brougher) Surfactant Protein B Expression in Avian Lung The avian lung, which is structurally similar to mammalian upper airway, has been suggested as a novel model for human upper respiratory disease. Of particular interest are the surfactant proteins. The human alveolar surfactant proteins (SP-A, SP-B, SP-C and SP-D) are secreted by type 2 cells and function to reduce alveolar surface tension and modulate a localized immune response. The composition and function of surfactant in the upper airway is less well understood, yet it likely prevents the collapse of the bronchioles, and mediates inflammatory responses. The avian parabronchi (tubules which serve as the site of gas exchange) contain type 2 cells and have been shown to express SP-A and SP-B. Beyond this, little research has been conducted on avian lung. The goals of this project are (1) to characterize expression of the surfactant proteins in avian lung, and identify the embryonic age at which upregulation of expression occurs; (2) develop an in vitro culture system for avian embryonic lung explant tissue, and (3) develop a model for the isolation and differentiation of avian type 2 cells

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Sabrina McGary（布劳格） 表面活性蛋白B在鸡肺组织中的表达 禽肺与哺乳动物的上呼吸道结构相似，是研究人类上呼吸道疾病的新模型。特别感兴趣的是表面活性剂蛋白。人肺泡表面活性蛋白（SP-A、SP-B、SP-C和SP-D）由2型细胞分泌，其功能是降低肺泡表面张力并调节局部免疫应答。 表面活性剂在上气道中的组成和功能尚不清楚，但它可能防止细支气管塌陷，并介导炎症反应。鸟类副支气管（作为气体交换部位的小管）含有2型细胞，并已显示表达SP-A和SP-B。 除此之外，对禽肺的研究很少。本研究的主要目的是：（1）研究肺表面活性蛋白在鸡胚肺组织中的表达，并确定表达上调的胚龄;（2）建立鸡胚肺组织的体外培养体系;（3）建立鸡胚肺2型细胞的分离和分化模型