DNA BARCODING AND IDENTIFICATION OF BLACK HILLS ARACHNIDS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 对南达科他州黑山地区的蜘蛛纲动物几乎一无所知。传统的蜘蛛纲鉴定需要训练有素的分类学家和特定地区的分类检索表。由于目前在南达科他州的黑山还没有这两种动物，因此对黑山的蜘蛛动物区系进行调查几乎是不可能的。本项目使用了一种不同的方法，最好的描述是“反向分类法”。人们正在收集来自黑山的蜘蛛类样本，然后对DNA进行采样，并对细胞色素氧化酶I线粒体基因的一个区域进行特征描述。然后，这些DNA条形码将与从DNA数据库(如GenBank)获得的有凭据的标本进行比较，或与从博物馆收藏的标本进行比较。这种DNA条形码方法不仅提供了一种分类鉴定的方法，而且也为识别新物种或所描述物种的新遗传形式提供了机会。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Virtually nothing is known about the arachnid fauna of the Black Hills region of South Dakota. Traditional identification of arachnids requires a trained systematist and taxonomic keys for a given region. Since neither of these is presently available for the Black Hills of South Dakota, a survey of the arachnid fauna in the Black Hills is virtually impossible. A different approach is being used for the present project, that could best be described as "reverse taxonomy." Arachnid samples from the Black Hills are being collected and then sampled for DNA and characterized for a region of the cytochrome oxidase I mitochondrial gene. These DNA barcodes will then be compared with vouchered specimens obtained either from DNA databases, such as genbank or with specimens obtained in collections from museums. This DNA barcoding approach not only provides a method of taxonomic identification, but also affords the opportunity to identify new species or novel genetic forms of described species.