DNA BARCODING AND IDENTIFICATION OF BLACK HILLS ARACHNIDS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 事实上，人们对南达科他州黑山地区的蛛形纲动物群一无所知。传统的蛛形纲动物鉴定需要一个训练有素的系统学家和一个给定区域的分类检索表。由于这两个都不是目前可用于南达科他州的黑山，调查的蛛形纲动物在黑山几乎是不可能的。本项目采用了一种不同的方法，最好称之为“反向分类法”。“正在收集来自黑山的蛛形纲动物样本，然后进行DNA采样，并对细胞色素氧化酶I线粒体基因的一个区域进行表征。然后，这些DNA条形码将与从DNA数据库（如genbank）获得的凭证标本或从博物馆收藏中获得的标本进行比较。这种DNA条形码方法不仅提供了一种分类鉴定的方法，而且还提供了鉴定新物种或所述物种的新遗传形式的机会。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Virtually nothing is known about the arachnid fauna of the Black Hills region of South Dakota. Traditional identification of arachnids requires a trained systematist and taxonomic keys for a given region. Since neither of these is presently available for the Black Hills of South Dakota, a survey of the arachnid fauna in the Black Hills is virtually impossible. A different approach is being used for the present project, that could best be described as "reverse taxonomy." Arachnid samples from the Black Hills are being collected and then sampled for DNA and characterized for a region of the cytochrome oxidase I mitochondrial gene. These DNA barcodes will then be compared with vouchered specimens obtained either from DNA databases, such as genbank or with specimens obtained in collections from museums. This DNA barcoding approach not only provides a method of taxonomic identification, but also affords the opportunity to identify new species or novel genetic forms of described species.