CONSERVATION GENETICS OF THE AMERICAN DIPPER IN THE BLACK HILLS OF SOUTH DAKOTA

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。该项目的长期目标是开发美洲北斗七星天牛的DNA指纹标记，可以用来确定黑山种群与该物种其他落基山脉种群的关系。1996年，美洲北斗七星被列入南达科他州濒危物种名单。这种小型水鸟的数量在南达科他州的所有S溪流中都在下降，除了鱼枪溪。潜在筑巢地点的丧失和溪流的筑坝导致了北斗七星种群的下降(Price和Bock，1983)。此外，溪流越来越多的沉积对水生昆虫幼虫不利，水生昆虫幼虫是美洲勺子的主要食物来源。该项目始于2002年1月，已经开发出非侵入性的方法，从黑山的勺子种群中获取DNA样本。这一点意义重大，因为需要在不进一步减少种群规模的情况下收集遗传数据。该项目的下一阶段是构建一个小片段基因组文库，并确定勺子基因组中的多态微卫星区域。然后，我们将利用这些区域开发遗传标记。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long-term objective of this project is to develop DNA fingerprinting markers for Cinclus mexicanus, the American dipper, that can be used to determine the relationship of the Black Hills populations with other Rocky Mountain populations of this species. In 1996 the American dipper was placed on the list of threatened species in South Dakota. The population of this small aquatic bird is in decline in all of South Dakota?s streams except for Spearfish Creek. Loss of potential nesting sites and damming of streams have contributed to the declining dipper populations (Price and Bock, 1983). Additionally, the increasing sedimentation of streams has been detrimental to aquatic insect larvae, the major food source of American dippers. The project, which began in January 2002, has developed noninvasive methods to obtain DNA samples from the Black Hills population of dippers. This is significant because of the need to gather genetic data without further reducing population size. The next phase of the project is to construct a small fragment genomic library and identify polymorphic microsatellite regions within the dipper genome. We would then develop genetic markers using those regions.