CELL TO CELL COMMUNICATION IN MYCOBACTERIA

分枝杆菌中的细胞间通讯

• 批准号: 7725201
• 负责人: ANGELA MCKINNEY-WILLIAMS
• 金额: $ 2.42万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7725201
• 关键词: B-Lymphocytes; Body cavities; Cell Communication; Cells; Computer Retrieval of Information on Scientific Projects Database; DNA; Dominant-Negative Mutation; Epstein-Barr virus LMP-1 protein; Funding; Genus Mycobacterium; Grant; Herpesviridae; Human; Human Herpesvirus 4; Human Herpesvirus 8; Immunoprecipitation; In Vitro; Institution; Malignant Neoplasms; Mammalian Cell; Membrane Proteins; Play; Process; Proteins; Purpose; Research; Research Personnel; Resources; Role; Signal Transduction; Signaling Molecule; Simplexvirus; Source; Testing; Transfection; United States National Institutes of Health; Work; body cavity; cell transformation; in vivo; research study; tumor; tumorigenesis; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In Dr. Zhang's lab I have continued researching the relationship between the Epstein Barr Virus and cancer. Epstein-Barr virus (EBV), a human herpesvirus, plays a critical role in oncogenesis of mammalian cells. The transformation process is primarily caused by the EBV latent membrane protein 1 (LMP-1). In vitro, EBV cannot transform primary B-cells without the expression of LMP-1. A dominant negative mutant of LMP-1 referred to as LMP-DM has been generated. The LMP-DM protein has the ability to inhibit cell signaling molecules which are normally regulated by LMP-1, specifically those involved in cancer formation . The purpose of this research was to test if LMP-DM has the ability to block the transformation function of LMP-1 in EBV transformed cells. A second project (May 2004-Dec2004) that I have worked on is looking at the relationship between the Human Herpesvirus 8 (HHV-8), Epstein Barr Virus (EBV), and body cavity tumor formation. Co-transfection experiments using vectors containing DNA from HHV-8 and EBV along with immunoprecipitation experiments were done to determine if HHV-8 and EBV cause tumor formation, specifically by causing the expression of tumor specific proteins in vivo.

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