CELL TO CELL COMMUNICATION IN MYCOBACTERIA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在张博士的实验室里，我一直在继续研究爱泼斯坦-巴尔病毒和癌症之间的关系。EB病毒(Epstein-Barr Virus，EBV)是一种人类疱疹病毒，在哺乳动物细胞的肿瘤发生中起重要作用。这一转化过程主要由EB病毒潜伏膜蛋白1(LMP-1)引起。在体外，EBV不能在不表达LMP-1的情况下转化原代B细胞。LMP-1的显性负性突变体被称为LMP-DM。LMP-DM蛋白有能力抑制通常由LMP-1调节的细胞信号分子，特别是那些参与癌症形成的分子。本研究的目的是检测LMP-DM在EBV转化细胞中是否具有阻断LMP-1转化功能的能力。我参与的第二个项目(2004年5月至2004年12月)是研究人类疱疹病毒8型(HHV-8)、爱泼斯坦-巴尔病毒(EBV)和体腔肿瘤形成之间的关系。用含有HHV-8和EBV的DNA的载体进行共转染实验，并进行免疫沉淀实验，以确定HHV-8和EBV是否会导致肿瘤的形成，特别是通过在体内引起肿瘤特异性蛋白的表达。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In Dr. Zhang's lab I have continued researching the relationship between the Epstein Barr Virus and cancer. Epstein-Barr virus (EBV), a human herpesvirus, plays a critical role in oncogenesis of mammalian cells. The transformation process is primarily caused by the EBV latent membrane protein 1 (LMP-1). In vitro, EBV cannot transform primary B-cells without the expression of LMP-1. A dominant negative mutant of LMP-1 referred to as LMP-DM has been generated. The LMP-DM protein has the ability to inhibit cell signaling molecules which are normally regulated by LMP-1, specifically those involved in cancer formation . The purpose of this research was to test if LMP-DM has the ability to block the transformation function of LMP-1 in EBV transformed cells. A second project (May 2004-Dec2004) that I have worked on is looking at the relationship between the Human Herpesvirus 8 (HHV-8), Epstein Barr Virus (EBV), and body cavity tumor formation. Co-transfection experiments using vectors containing DNA from HHV-8 and EBV along with immunoprecipitation experiments were done to determine if HHV-8 and EBV cause tumor formation, specifically by causing the expression of tumor specific proteins in vivo.