KANSAS U COBRE: PREIMPLANTATION RELEASED PROTEINS EMBRYO QUALITY PREDICTORS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 目前，美国每年出生的儿童中约有1%是通过辅助生殖技术(ART)受孕的，在过去20年里，全球已有100多万儿童受孕和分娩。通过人工授精受孕的孩子更有可能表现出低出生体重和早产。ART引起的大部分发病率是由于这种治疗相关的高多胎妊娠率。到目前为止，胚胎质量评估是基于体外培养后胚胎的主观形态评估。选择高质量胚胎的客观标准应提高ART成功率，并使单胚胎移植成为更可行的治疗方案。因此，有一个令人信服的案例来开发预测胚胎质量的分析性和非侵入性检测方法。这项研究的长期目标是将植入前早期胚胎中发生的分子、细胞和生化过程与最终导致成功怀孕的发育事件联系起来。这项研究的假设是，早期胚胎分泌/释放反映胚胎发育能力的蛋白质。这项建议将首次在条件培养液中集体鉴定早期胚胎分泌/释放的蛋白质标记，以开始建立指示胚胎质量的蛋白质的特征/模式。目的1利用串联质谱仪从质量不同的小鼠胚胎中鉴定分泌/释放蛋白(S)。目的2将验证这些蛋白质是否可以用于预测体内胚胎质量，以及小鼠系统是否可移植到人类系统。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Presently, ~1% of children born each year in the USA are conceived via assisted reproductive technologies (ART) and world-wide more than 1 million children have been conceived and delivered over the last 2 decades. Children conceived through ART are more likely to exhibit low birth weights and to be delivered prematurely. Much of the morbidity that stems from ART is due to the high rate of multiple gestations associated with this treatment. To date, embryo quality assessments are based on a subjective morphological evaluation of the embryo following in vitro culture. Objective criteria for selection of high quality embryos should increase ART success and make single embryo transfer a more viable therapeutic option. Thus, there is a compelling case for the development of analytical and non-invasive assays that predict embryo quality. The long-range goal of this research is to link molecular, cellular and biochemical processes occurring in the early embryo prior to implantation to developmental events that ultimately result in a successful pregnancy. The hypothesis of the proposed research is that the early embryo secretes/releases proteins that reflect the developmental competence of that embryo. This proposal will identify en masse for the first time early embryonic secreted/released protein markers in conditioned medium to begin to establish a signature/pattern of proteins indicative of embryo quality. Aim 1 will identify secreted/released protein(s) from murine embryos exhibiting qualitative differences using tandem mass spectrometry. Aim 2 will validate whether these proteins can be used to predict in vivo embryo quality and whether the murine system is translatable to the human system.