KANSAS U COBRE: PREIMPLANTATION RELEASED PROTEINS EMBRYO QUALITY PREDICTORS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目前，每年在美国出生的儿童中约有1%是通过辅助生殖技术（ART）受孕的，在过去的20年里，全世界已有超过100万名儿童被受孕和分娩。通过抗逆转录病毒疗法受孕的儿童更有可能出现出生体重不足和早产。抗逆转录病毒疗法引起的大部分发病率是由于与这种治疗相关的多胎妊娠率很高。迄今为止，胚胎质量评估是基于体外培养后胚胎的主观形态学评价。 选择高质量胚胎的客观标准应该增加ART的成功率，并使单胚胎移植成为一种更可行的治疗选择。 因此，有一个令人信服的情况下，预测胚胎质量的分析和非侵入性测定的发展。这项研究的长期目标是将植入前早期胚胎中发生的分子，细胞和生化过程与最终导致成功怀孕的发育事件联系起来。这项研究的假设是，早期胚胎分泌/释放反映胚胎发育能力的蛋白质。 该建议将首次鉴定条件培养基中早期胚胎分泌/释放的蛋白质标记物，以开始建立指示胚胎质量的蛋白质的特征/模式。 目的1将使用串联质谱法鉴定来自小鼠胚胎的分泌/释放的蛋白质，这些蛋白质表现出定性差异。 目的2将验证这些蛋白质是否可用于预测体内胚胎质量，以及小鼠系统是否可转化为人类系统。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Presently, ~1% of children born each year in the USA are conceived via assisted reproductive technologies (ART) and world-wide more than 1 million children have been conceived and delivered over the last 2 decades. Children conceived through ART are more likely to exhibit low birth weights and to be delivered prematurely. Much of the morbidity that stems from ART is due to the high rate of multiple gestations associated with this treatment. To date, embryo quality assessments are based on a subjective morphological evaluation of the embryo following in vitro culture. Objective criteria for selection of high quality embryos should increase ART success and make single embryo transfer a more viable therapeutic option. Thus, there is a compelling case for the development of analytical and non-invasive assays that predict embryo quality. The long-range goal of this research is to link molecular, cellular and biochemical processes occurring in the early embryo prior to implantation to developmental events that ultimately result in a successful pregnancy. The hypothesis of the proposed research is that the early embryo secretes/releases proteins that reflect the developmental competence of that embryo. This proposal will identify en masse for the first time early embryonic secreted/released protein markers in conditioned medium to begin to establish a signature/pattern of proteins indicative of embryo quality. Aim 1 will identify secreted/released protein(s) from murine embryos exhibiting qualitative differences using tandem mass spectrometry. Aim 2 will validate whether these proteins can be used to predict in vivo embryo quality and whether the murine system is translatable to the human system.