KANSAS U COBRE: PREIMPLANTATION RELEASED PROTEINS EMBRYO QUALITY PREDICTORS

堪萨斯大学 COBRE：植入前释放的蛋白质胚胎质量预测因子

• 批准号: 8167981
• 负责人: LANE K. CHRISTENSON
• 金额: $ 22万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167981
• 关键词: Assisted Reproductive Technology; Biochemical Process; Biological Assay; Child; Competence; Computer Retrieval of Information on Scientific Projects Database; Conditioned Culture Media; Development; Embryo; Embryo Transfer; Evaluation; Event; Exhibits; Funding; Goals; Grant; Human; In Vitro; Institution; Kansas; Link; Low Birth Weight Infant; Molecular; Morbidity - disease rate; Mus; Pattern; Pregnancy; Proteins; Research; Research Personnel; Resources; Selection Criteria; Source; System; Therapeutic; Time; United States National Institutes of Health; base; embryonic protein; implantation; in vivo; preimplantation; stem; success; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Presently, ~1% of children born each year in the USA are conceived via assisted reproductive technologies (ART) and world-wide more than 1 million children have been conceived and delivered over the last 2 decades. Children conceived through ART are more likely to exhibit low birth weights and to be delivered prematurely. Much of the morbidity that stems from ART is due to the high rate of multiple gestations associated with this treatment. To date, embryo quality assessments are based on a subjective morphological evaluation of the embryo following in vitro culture. Objective criteria for selection of high quality embryos should increase ART success and make single embryo transfer a more viable therapeutic option. Thus, there is a compelling case for the development of analytical and non-invasive assays that predict embryo quality. The long-range goal of this research is to link molecular, cellular and biochemical processes occurring in the early embryo prior to implantation to developmental events that ultimately result in a successful pregnancy. The hypothesis of the proposed research is that the early embryo secretes/releases proteins that reflect the developmental competence of that embryo. This proposal will identify en masse for the first time early embryonic secreted/released protein markers in conditioned medium to begin to establish a signature/pattern of proteins indicative of embryo quality. Aim 1 will identify secreted/released protein(s) from murine embryos exhibiting qualitative differences using tandem mass spectrometry. Aim 2 will validate whether these proteins can be used to predict in vivo embryo quality and whether the murine system is translatable to the human system.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 目前，美国每年出生的儿童约有 1% 是通过辅助生殖技术 (ART) 受孕的，过去 20 年来，全世界已有超过 100 万儿童受孕和分娩。通过 ART 怀上的孩子更有可能表现出低出生体重和早产。 ART 引起的大部分发病率是由于与这种治疗相关的高多胎妊娠率所致。迄今为止，胚胎质量评估是基于体外培养后对胚胎的主观形态学评估。 选择高质量胚胎的客观标准应该会提高 ART 的成功率，并使单胚胎移植成为更可行的治疗选择。 因此，开发预测胚胎质量的分析和非侵入性检测方法是有说服力的。这项研究的长期目标是将植入前早期胚胎中发生的分子、细胞和生化过程与最终导致成功妊娠的发育事件联系起来。拟议研究的假设是早期胚胎分泌/释放反映该胚胎发育能力的蛋白质。 该提案将首次在条件培养基中集体鉴定早期胚胎分泌/释放的蛋白质标记，以开始建立指示胚胎质量的蛋白质特征/模式。 目标 1 将使用串联质谱法鉴定鼠胚胎中表现出质量差异的分泌/释放蛋白质。 目标 2 将验证这些蛋白质是否可用于预测体内胚胎质量以及小鼠系统是否可转化为人类系统。