HUMORAL IMMUNITY TO A NOVEL PNEUMOCYSTIS CARINII ANTIGEN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目旨在消除p55-v3和p55-v0抗原的比较亚细胞共定位研究，因为未能制造出针对这两种抗原的特异性抗体。我们提出了以下具体目标： 具体目标1： 研究p55-v3抗原的生化性质和亚细胞定位。 特异性目的2：检测实验性感染大鼠血清中对p55-v3和p55-v0的抗体反应。 具体目的3：探讨p55-v3对重组p55-v3抗原主动免疫大鼠的保护作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The project aims to eliminate the comparative subcellular co-localiation studies of p55-v3 and p55-v0 antigens due to the failure at making antibodies specific for either antigens. We proposed the following specific aims: Specific Aim 1: To characterize the biochemical nature and subcellular location of the p55-v3 antigen. Specific Aim 2: To examine the serum antibody responses to p55-v3 and p55-v0 during experimental infection in rats. Specific Aim 3: To investigate the protective efficacy of p55-v3 in rats following active immunization with recombinant p55-v3 antigen.