HUMORAL IMMUNITY TO A NOVEL PNEUMOCYSTIS CARINII ANTIGEN

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目旨在消除p55-v3和p55-v0抗原的比较亚细胞共定位研究，因为未能制备对任一抗原特异的抗体。我们提出了以下具体目标： 具体目标1： 表征p55-v3抗原的生化性质和亚细胞定位。 目的二：检测大鼠实验性感染p55-v3和p55-v0的血清抗体反应。 目的3：研究重组p55-v3抗原主动免疫大鼠后p55-v3的保护作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The project aims to eliminate the comparative subcellular co-localiation studies of p55-v3 and p55-v0 antigens due to the failure at making antibodies specific for either antigens. We proposed the following specific aims: Specific Aim 1: To characterize the biochemical nature and subcellular location of the p55-v3 antigen. Specific Aim 2: To examine the serum antibody responses to p55-v3 and p55-v0 during experimental infection in rats. Specific Aim 3: To investigate the protective efficacy of p55-v3 in rats following active immunization with recombinant p55-v3 antigen.